Fine tuning of the photosystem II major antenna mobility within the thylakoid membrane of higher plants

Daskalakis, Vangelis; Papadatos, Sotiris; Kleinekathöfer, Ulrich

doi:10.1016/j.bbamem.2019.183059

Cited by 36 publications

(40 citation statements)

References 63 publications

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“…Based on our experimental results, we may extend earlier models describing the PsbS function in vivo interacting with key constituents of the thylakoid membrane 19,50,51 . It has been established that in vivo , PsbS interacts with LHCs under high light conditions 8,24,52,53 .…”

Section: Mainsupporting

confidence: 63%

“…Strikingly, the symmetry-related luminal amphipathic helices of LHC contain several titratable residues (Asp, Glu) 54–57 which may enable such functionality. 51 Thus, responsiveness of the amphipathic helices to changes in pH, hydrophobicity or other alterations in the physico-chemical environment could be a common motif that enables LHCs to operate as molecular controls for regulating photosynthetic light harvesting.…”

Section: Mainmentioning

confidence: 99%

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The molecular pH-response mechanism of the plant light-stress sensor PsbS

Krishnan-Schmieden

Konold

Kennis

et al. 2018

Preprint

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The membrane protein Photosystem II subunit S (PsbS) is a pH sensor that plays an essential role in signaling light stress in plants to prevent photo oxidation and generation of detrimental reactive species. PsbS detects thylakoid lumen acidification in excess light conditions via two glutamates facing the lumen, however, its molecular mechanism for activation is elusive. We performed a spectroscopic analysis of wild type Physcomitrella patens PsbS and of mutants in which the active glutamates have been replaced (E69Q, E174Q and E69Q / E174Q). We discovered that E69 exerts allosteric control of PsbS dimerization, while E174 is essential for the secondary structural response to low pH. Based on our results, we propose a molecular pH response mechanism that involves a change in the amphiphatic short helix containing E174 facing the lumen, moving from the aqeuous phase into the hydrophobic membrane phase. This structural mechanism may be a shared motif of protein molecular switches of the light-harvesting family and its elucidation could open new routes for crops engineering to improve photosynthetic production of biomass.

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Section: Mainsupporting

confidence: 63%

Section: Mainmentioning

confidence: 99%

The molecular pH-response mechanism of the plant light-stress sensor PsbS

Krishnan-Schmieden

Konold

Kennis

et al. 2018

Preprint

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“…It is likely, however, that the thylakoid membrane thinning triggered by ΔpH (Johnson et al , ) causes hydrophobic mismatch between LHCII and the membrane bilayer that thermodynamically drives the changes in LHCII that are facilitated by PsbS. The dynamics of this protein interaction with membrane lipids has been recently proposed as a key mechanism (Daskalakis et al , ).…”

Section: Molecular Basis For Npq (Qe)mentioning

confidence: 99%

Dynamic non‐photochemical quenching in plants: from molecular mechanism to productivity

2019

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Summary Photoprotection refers to a set of well defined plant processes that help to prevent the deleterious effects of high and excess light on plant cells, especially within the chloroplast. Molecular components of chloroplast photoprotection are closely aligned with those of photosynthesis and together they influence productivity. Proof of principle now exists that major photoprotective processes such as non‐photochemical quenching (NPQ) directly determine whole canopy photosynthesis, biomass and yield via prevention of photoinhibition and a momentary downregulation of photosynthetic quantum yield. However, this phenomenon has neither been quantified nor well characterized across different environments. Here we address this problem by assessing the existing literature with a different approach to that taken previously, beginning with our understanding of the molecular mechanism of NPQ and its regulation within dynamic environments. We then move to the leaf and the plant level, building an understanding of the circumstances (when and where) NPQ limits photosynthesis and linking to our understanding of how this might take place on a molecular and metabolic level. We argue that such approaches are needed to fine tune the relevant features necessary for improving dynamic NPQ in important crop species.

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“…The local environment of membrane proteins has also a strong impact on their mobility and controls their tendency to interact with other proteins ( Killian, 1998 ; Quemeneur et al., 2014 ). Recently, it has been hypothesized that a reorganization of the lipids bound to LHCII is associated with a hydrophobic mismatch around the complexes, and this in turn locks the dissipative state of the antennae in the thylakoid membrane ( Daskalakis et al., 2019 ; Ruban, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

A Protein Environment-Modulated Energy Dissipation Channel in LHCII Antenna Complex

et al. 2020

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Summary The major light-harvesting complex of photosystem II (LHCII) is the main contributor to sunlight energy harvesting in plants. The flexible design of LHCII underlies a photoprotective mechanism whereby this complex switches to a dissipative state in response to high light stress, allowing the rapid dissipation of excess excitation energy (non-photochemical quenching, NPQ). In this work, we locked single LHCII trimers in a quenched conformation after immobilization of the complexes in polyacrylamide gels to impede protein interactions. A comparison of their pigment excited-state dynamics with quenched LHCII aggregates in buffer revealed the presence of a new spectral band at 515 nm arising after chlorophyll excitation. This is suggested to be the signature of a carotenoid excited state, linked to the quenching of chlorophyll singlet excited states. Our data highlight the marked sensitivity of pigment excited-state dynamics in LHCII to structural changes induced by the environment.

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Fine tuning of the photosystem II major antenna mobility within the thylakoid membrane of higher plants

Cited by 36 publications

References 63 publications

The molecular pH-response mechanism of the plant light-stress sensor PsbS

The molecular pH-response mechanism of the plant light-stress sensor PsbS

Dynamic non‐photochemical quenching in plants: from molecular mechanism to productivity

A Protein Environment-Modulated Energy Dissipation Channel in LHCII Antenna Complex

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