Fingerprint profiling of acid hydrolyzates of polysaccharides extracted from the fruiting bodies and spores of Lingzhi by high-performance thin-layer chromatography

Di, Xin; Chan, Kuen; Leung, Hei Wun; Huie, Carmen W.

doi:10.1016/j.chroma.2003.07.015

Cited by 110 publications

(54 citation statements)

References 22 publications

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“…SFE and supercritical fluid chromatography were also utilized in flour and moldy bread for ergosterol detection as indicator of fungal contamination (Young & Games, 1993). PLE was utilized for the selective isolation of polysaccharides from several fungal species (Fan, Li, Chu, Zhang & Yan, 2012;Palanisamy et al, 2012;Di, Chan, Leung & Huie, 2003;Lo, Tsao, Wang & Chang, 2007) and certain fatty acids from Cordyceps spp. (Yang, Feng, Zhao & Li, 2009).…”

Section: Introductionmentioning

confidence: 99%

Sterol enriched fractions obtained from Agaricus bisporus fruiting bodies and by-products by compressed fluid technologies (PLE and SFE)

Gil-Ramírez¹,

Aldars-García²,

Marimuthu³

et al. 2013

Innovative Food Science & Emerging Technologies

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Ergosterol, ergosta7,22 dienol, ergosta 5,7 dienol, fungisterol,7,6,8(14),22-tetraen-3-ona were the fungal sterols detected in Agaricus bisporus mushrooms after optimization of an sterol extraction method. Their concentration ranged from 3.1-11.2 mg/g dw depending on the strain, casing soil, flush number, developmental stage and sporophore tissue analyzed. Two methods were optimized to obtain sterols enriched extracts from A. bisporus fruiting bodies using pressurized fluid (PLE) and supercritical fluid extractions (SFE). PLE using ethanol as solvent at 10.7 MPa (50ºC and 100ºC) after 5 cycles of 5 min extraction (mixing in the extraction cell the sample with sand in a ratio 1:4) yielded extracts with respect. 5 and 2.9% sterols. Using SFE-CO2 at 40ºC and 9 to 30 MPa fractions containing 60% of sterols were obtained. Both technologies could be also utilized to extract sterols from mushroom by-products (the lower part of the stipe) as a method for their valorization. IntroductionCardiovascular diseases (CVD) are one of the leading causes of death in industrialized countries. Although, up to 80% of the cases could be prevented by changing style life, advertises of public agencies do not have the impact they should.Nowadays, only the consumption of functional foods with hypocholesterolemic properties seems to be better accepted by the CVD risk population than other changes.The functional foods, available at the markets, bearing the claim `able to reduce cholesterol levels in serum´ contain mainly plant phytosterol (Ling & Jones, 1995;Turnham, 1999), or cereal β-glucans. The mechanism of action of the hypocholesterolemic β-glucans is not completely elucidated (Cheickna & Hui, 2012) while apparently the phytosterols are able to impair cholesterol absorption by displacing Edible mushrooms also contain sterols with structural similarities to phytosterols and cholesterol. Thus, these fungal molecules might also act as phytosterols displacing cholesterol from the DMMs. Ergosterol (ergosta-5,7,22-trien-3β-ol) is the major sterol of their hyphae membranes (approx. 80% of the sterols w/w) followed by other 4 derivatives such as ergosta-5,8,22-trien-3-ol, ergosta-7,22-dien-3-ol, ergosta-5,7-dien-3-ol and ergosta-7-en-3-ol In this work, ergosterol and derivatives were measured in several Agaricus bisporus varieties during different cultivation parameters, developmental stages, sporophore tissues etc. The sterols were extracted by both SFE and PLE technologies in order to compare them and define the optimal method and extraction parameters to obtain sterols-enriched fractions. Extractions were carried out using the complete fruiting body or the lower part of the stipe usually discarded as a by-product during harvesting to investigate the possible revalorization of this by-product as starting material to generate ergosterol-enriched fractions for novel functional ingredients with hypocholesterolemic properties. stage was studied). In some cases, the different sporophore tissues, including the lower part of the stipe...

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Section: Introductionmentioning

confidence: 99%

Sterol enriched fractions obtained from Agaricus bisporus fruiting bodies and by-products by compressed fluid technologies (PLE and SFE)

Gil-Ramírez¹,

Aldars-García²,

Marimuthu³

et al. 2013

Innovative Food Science & Emerging Technologies

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“…Traditional Chinese Medicine (TCM) is based on the synergic effect on its complex components, which is different from western medicine [17][18][19][20]. The quality control methods of herbal medicine almost depend on selecting one or some of components as reference indexes, which cannot represent the whole property of herbal medicine just by several so-called index components [16].…”

Section: Analysis Of the Resultsmentioning

confidence: 99%

Simultaneous determination of paeoniflorin and albiflorin in radix paeoniae rubra by HPLC—DAD—ELSD

Zhu

You³

et al. 2017

Acta Chromatographica

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Summary. High-performance liquid chromatography coupled with photodiode array detection and evaporative light scattering detection (HPLC-DAD-ELSD) was established to determine paeoniflorin and albiflorin simultaneously in Radix Paeoniae Rubra. The assay was performed on a Diamonsil C 18 (4.6 mm × 250 mm, 5 μm) column by a gradient elution program with acetonitrile and aqueous formic acid (0.05% v/v) as mobile phase at a flow rate of 1.0 mL min −1 . The detection wavelength of DAD was 230 nm, and the evaporator tube temperature of ELSD was set at 110 °C with the nebulizing gas flow rate of 3 L min −1 . The temperature of column was kept at 30 °C. The linear ranges of paeoniflorin and albiflorin were within 0.050-1.510 mg mL −1 and 1.007-5.035 mg mL −1 . The recoveries of paeoniflorin and albiflorin were 96.2-102.9% and 95.0-102.4%, respectively, while the relative standard deviation (RSD) of them was 0.2-2.5%. This method was quick, simple, accurate, and specific. It could be used for the quality control of Radix Paeoniae Rubra. The proposed approach was expected as a powerful tool for the quality control of Radix Paeoniae Rubra.

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“…The chromatographic method was validated according to ICH guidelines. Linearity study indicated that area was directly proportional to concentration (r 2 Previous study has reported that quantitative analysis of andrographolide in Andrographis paniculata herb samples by highperformance thin-layer chromatographic methods [31]. The whole plant of Andrographis echioides contains more number of phytoconstituents (alkaloids, flavonoids, glycosides, phenols, phytosterols, proteins, saponins, tannins and triterpenoids, volatileoils, amino acid, cardiac glycosides, gums and phytosteroids) that are extracted using various solvents depending upon the polarity of these compounds [32,33].…”

Section: Discussionmentioning

confidence: 99%

“…HPTLC is a widely accepted technique for its high accuracy, precision and reproducibility of results. In addition, HPTLC has many advantages because of high sample throughput at low operating cost, easy sample preparation, short analysis time and analytical assurance [2][3][4].…”

Section: Introductionmentioning

confidence: 99%

HPTLC Method for the Determination of Lupeol From Andrographis Echioides Leaves

Gurupriya¹,

Cathrine²,

Pratheema³

2018

Int J Pharm Pharm Sci

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Objective: A new simple, precise, rapid and selective high -performance thin-layer chromatographic (HPTLC) method has been developed for th e determination of lupeol in methanolic leaves extract of Andrographis echioides leavesResults: The retention factor of lupeol was found to be 0.55. Linearity was obtained in the range of 5000 ng -10000 ng for lupeol. The developed and validated HPTLC method was employed for lupeol in methanolic leaves extract of Andrographis echioides for standardization of the content of the marker. The linear regression data for the calibration plots showed a good linear relationship with r=0.99917 for lupeol. Satisfactory recoveries of 99.80 % were obtained for lupeol.. The concentration of standard lupeol was 1 mg/ml. Conclusion:The results obtained in validation assays indicate the accuracy and reliability of the developed HPTLC method for the quantification of lupeol in methanolic leaves extract of Andrographis echioides.

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Fingerprint profiling of acid hydrolyzates of polysaccharides extracted from the fruiting bodies and spores of Lingzhi by high-performance thin-layer chromatography

Cited by 110 publications

References 22 publications

Sterol enriched fractions obtained from Agaricus bisporus fruiting bodies and by-products by compressed fluid technologies (PLE and SFE)

Sterol enriched fractions obtained from Agaricus bisporus fruiting bodies and by-products by compressed fluid technologies (PLE and SFE)

Simultaneous determination of paeoniflorin and albiflorin in radix paeoniae rubra by HPLC—DAD—ELSD

HPTLC Method for the Determination of Lupeol From Andrographis Echioides Leaves

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