First data on Pneumocystis jirovecii colonization in patients with respiratory diseases in North Lebanon

Khalife, Sara; Aliouat, El Moukhtar; Aliouat-Denis, Cécile-Marie; Gantois, Nausicâa; Devos, Patrick; Mallat, Hassan; Dei‐Cas, Eduardo; Dabboussi, Fouad; Hamzé, Monzer; Fréalle, Émilie

doi:10.1016/j.nmni.2015.02.006

Cited by 14 publications

(14 citation statements)

References 19 publications

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“…In the same year at other hospitals in Indonesia (RS Persahabatan and the RSCM), 14.55% of the PjP was found in 55 patients studied. The figure is lower than the state other countries 6,7 .…”

Section: Ic-biolismentioning

confidence: 61%

Detection of Opportunistic Fungus Pneumocystis jirovecii Major Surface Glycoprotein (MSG) gene in HIV-AIDS Patients with Pneumoniae in Jakarta

Tjampakasari

Yasmon

Sjahrurachman

et al. 2020

KLS

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Pneumocystis jirovecii is known to cause opportunistic infections in the lower respiratory tract in individuals with low immune systems, especially patient with HIV infection. The prevalence of P. jirovecii pneumonia (PjP) in various countries show varying numbers. In Indonesia, HIV cases continue to rise. However, the data in Indonesia concerning the case of PjP is very limited. Until now the prevalence of PjP in Indonesia is only based on clinical symptoms of the patient. Currently, diagnosis of PjP relies on microscopic examination. The disadvantage of this examination is not easy to do and has a high negative predictive value. Thus, this study was conducted to develop a molecular test to diagnose PjP infection in HIV-AIDS suspected pneumonia. Molecular diagnostic test aimed for Major Surface Glycoprotein (MSG) gene of P. jirovecii detection was done through real-time PCR against 100 sputum samples. Demographic data show that the prevalence of PjP infection in HIV-AIDS suspected pneumonia patients in Jakarta is 20.0%, male 75% within 31-40 y.o (35%), dominant (80%) from patients with CD4+ T-lymphocytes of 200-349 cells/µL. Molecular real-time PCR methods were shown to give five times sensitivity higher than Giemsa stain. Keywords: P. jirovecii, HIV, real-time PCR

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Section: Ic-biolismentioning

confidence: 61%

Detection of Opportunistic Fungus Pneumocystis jirovecii Major Surface Glycoprotein (MSG) gene in HIV-AIDS Patients with Pneumoniae in Jakarta

Tjampakasari

Yasmon

Sjahrurachman

et al. 2020

KLS

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show abstract

“…A high-level prevalence of P. jirovecii colonization in immunocompetent individuals has been reported in numerous studies throughout the world [2,29]. The role of P. jirovecii colonization in certain pulmonary diseases, particularly amongst patients with COPD, has gained increasing attention [5,8,9,30]. In the present multicenter study involving 403 non-HIV patients with various pulmonary diseases, we found a high prevalence of P. jirovecii colonization and this was associated with some clinical and laboratory characteristics of patients with pulmonary diseases, supporting the involvement of P. jirovecii colonization in pulmonary diseases.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, asymptomatic P. jirovecii colonization is being increasingly identified in immunocompetent individuals [2,8]. In recent years, the role of P. jirovecii colonization in pulmonary diseases has become a popular focus, especially in those with chronic obstructive pulmonary disease (COPD) and sudden infant death syndrome [5,[8][9][10][11]. These asymptomatic PJP individuals, carrying P. jirovecii, may act as an important reservoirs of nosocomial infections.…”

Section: Introductionmentioning

confidence: 99%

Pneumocystis jirovecii colonization and its association with pulmonary diseases: a multicenter study based on a modified loop-mediated isothermal amplification assay

Xue

Fan

et al. 2020

BMC Pulm Med

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Background: Pneumocystis jirovecii (P. jirovecii) is an opportunistic fungal pathogen and the role of its colonization in pulmonary diseases has become a popular focus in recent years. The aim of this study was to develop a modified loop-mediated isothermal amplification (LAMP) assay for detection of Pneumocystis jirovecii (P. jirovecii) DNA amongst non-HIV patients with various pulmonary diseases and use it to examine the prevalence and assess the association of P. jirovecii colonization with clinical characteristics of these diseases. Methods: We modified the previously reported LAMP assay for P. jirovecii by adding real-time detection. This method was used to detect P. jirovecii colonization in pulmonary samples collected from 403 non-HIV patients with various pulmonary diseases enrolled from 5 hospitals in China. We determined the prevalence of P. jirovecii colonization in 7 types of pulmonary diseases and assessed the association of P. jirovecii colonization with clinical characteristics of these diseases. Results: The modified LAMP assay showed no cross-reactivity with other common pulmonary microbes and was 1000 times more sensitive than that of conventional PCR. Using the modified LAMP assay, we detected P. jirovecii colonization in 281 (69.7%) of the 403 patients enrolled. P. jirovecii colonization was more common in interstitial lung diseases than in chronic obstructive pulmonary disease (COPD) (84.6% vs 64.5%, P < 0.05). Patients with acute exacerbation of COPD had a higher prevalence of P. jirovecii colonization compared to patients with stabilized COPD (67.4% vs 43.3%, P < 0.05). P. jirovecii colonization was associated with decreased pulmonary function, increased levels of 1,3-β-D-glucan and C-reactive protein, and decreased levels of CD4+ T-cell counts (P < 0.05 for each). Approximately 70% of P. jirovecii colonized patients had confections with other fungi or bacteria.

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“…jirovecii colonization in immunocompetent individuals has been reported in numerous studies throughout the world [2,29]. The role of P. jirovecii colonization in certain pulmonary diseases, particularly amongst patients with COPD, has gained increasing attention [5,8,9,30]. In the present multicenter study involving 403 non-HIV patients with various pulmonary diseases, we found a high prevalence of P. jirovecii colonization and this was associated with some clinical and laboratory characteristics of patients with pulmonary diseases, supporting the involvement of P. jirovecii colonization in pulmonary diseases.…”

Section: Association Of P Jirovecii Colonization With Clinical Charamentioning

confidence: 99%

“…jirovecii colonization is being increasingly identified in immunocompetent individuals [2,8]. In recent years, the role of P. jirovecii colonization in pulmonary diseases has become a popular focus, especially in those with chronic obstructive pulmonary disease (COPD) and sudden infant death syndrome [5,[8][9][10][11]. These asymptomatic PJP individuals, carrying P. jirovecii, may act as an important reservoirs of nosocomial infections.…”

Section: Introductionmentioning

confidence: 99%

Pneumocystis jirovecii Colonization and Its Association with Pulmonary Diseases: a Multicenter Study Based on a Modified Loop-Mediated Isothermal Amplification Assay

Xue¹,

Liu

et al. 2020

Preprint

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Background Pneumocystis jirovecii (P. jirovecii) is an opportunistic fungal pathogen and the role of its colonization in pulmonary diseases has become a popular focus in recent years. The aim of this study was to develop a modified loop-mediated isothermal amplification (LAMP) assay for detection of Pneumocystis jirovecii (P. jirovecii) DNA amongst non-HIV patients with various pulmonary diseases and use it to examine the prevalence and assess the association of P. jirovecii colonization with clinical characteristics of these diseases. Methods We modified the previously reported LAMP assay for P. jirovecii by adding real-time detection. This method was used to detect P. jirovecii colonization in pulmonary samples collected from 403 non-HIV patients with various pulmonary diseases enrolled from 5 hospitals in China. We determined the prevalence of P. jirovecii colonization in 7 types of pulmonary diseases and assessed the association of P. jirovecii colonization with clinical characteristics of these diseases. Results The modified LAMP assay showed no cross-reactivity with other common pulmonary microbes and was 1,000 times more sensitive than that of conventional PCR. Using the modified LAMP assay, we detected P. jirovecii colonization in 281 (69.7%) of the 403 patients enrolled. P. jirovecii colonization was more common in interstitial lung diseases than in chronic obstructive pulmonary disease (COPD) (84.6% vs 64.5%, P < 0.05). Patients with acute exacerbation of COPD had a higher prevalence of P. jirovecii colonization compared to patients with stabilized COPD (67.4% vs 43.3%, P < 0.05). P. jirovecii colonization was associated with decreased pulmonary function, increased levels of 1,3-β-D-glucan and C-reactive protein, and decreased levels of CD4+ T-cell counts (P < 0.05 for each). Approximately 70% of P. jirovecii colonized patients had confections with other fungi or bacteria. Conclusions We developed a modified LAMP assay for detecting P. jirovecii . Our multi-center study of 403 patients supports that P. jirovecii colonization is a risk factor for the development of pulmonary diseases and highlights the need to further study the pathogenesis and transmission of P. jirovecii colonization in pulmonary diseases.

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First data on Pneumocystis jirovecii colonization in patients with respiratory diseases in North Lebanon

Cited by 14 publications

References 19 publications

Detection of Opportunistic Fungus Pneumocystis jirovecii Major Surface Glycoprotein (MSG) gene in HIV-AIDS Patients with Pneumoniae in Jakarta

Detection of Opportunistic Fungus Pneumocystis jirovecii Major Surface Glycoprotein (MSG) gene in HIV-AIDS Patients with Pneumoniae in Jakarta

Pneumocystis jirovecii colonization and its association with pulmonary diseases: a multicenter study based on a modified loop-mediated isothermal amplification assay

Pneumocystis jirovecii Colonization and Its Association with Pulmonary Diseases: a Multicenter Study Based on a Modified Loop-Mediated Isothermal Amplification Assay

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