The Puccinia striiformis f. sp. tritici (Pst) pathotype, 134 E16A?, detected in 2002 in Australia, produced relatively lower and higher adult plant stripe rust responses, respectively, on cultivars Kukri and Janz in comparison to the pre-2002 Pst pathotype 110 E143A?. Molecular mapping of adult plant stripe rust response variation among 180 Kukri/Janz-derived doubled haploid lines over 4 years, two each with Pst pathotypes 110 E143A? and 134 E16A?, was performed. QYr.sun-7B and QYr.sun-7D were consistently contributed by Kukri and Janz, respectively. QYr.sun-7D corresponded to the genomic location of Yr18 and QYr.sun-7B remains to be formally named. QYr.sun-1B, QYr.sun-5B, and QYr.sun-6B were detected during more than one season irrespective of the Pst pathotypes used, whereas QYr.sun-3B was identified only during the 2003 crop season. QYr.sun-1A contributed by Janz, and QYr.sun-2A from Kukri, were detected only against Pst pathotypes 110 E143A? and 134 E16A?, respectively. The DH lines showing better resistance than the either parent carried combinations of 4 to 6 QTL. These lines are currently being used as stripe rust resistance donors in wheat breeding programs.