First Isolation of Metallo-β-Lactamase-Producing Multiresistant
            <i>Klebsiella pneumoniae</i>
            from a Patient in Brazil

Lincopán, Nilton; McCulloch, John Anthony; Reinert, Cristina; Cassettari, Valéria; Gales, Ana Cristina; Mamizuka, Elsa Masae

doi:10.1128/jcm.43.1.516-519.2005

Cited by 87 publications

(60 citation statements)

References 22 publications

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“…17 In Brazil, there has been some reports of multi-drug resistant hospital-acquired K. Pneumoniae. 18,19 In our study, 71 (84%) were ESBL positive by the double-disk synergy test and the Etest® and this value is considered high. This highlights the importance of investigating resistance mechanisms in hospitals.…”

Section: Discussionmentioning

confidence: 99%

Diversity of genotypes in CTX-M-producing Klebsiella pneumoniae isolated in different hospitals in Brazil

Chagas¹,

Alves²,

Vallim³

et al. 2011

Braz J Infect Dis

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Objective: The present study was undertaken to characterize CTX-M ESBL-producing Klebsiella pneumoniae collected from hospitals in different cities of Brazil. Material and Methods: Eighty-five K. pneumoniae strains isolated from hospitalized patients in six different hospitals of three cities of Brazil were analyzed. ESBL production was confirmed by the standard double-disk synergy test and the Etest®. The MIC 50 and MIC 90 for ESBL-producing isolates were determined by the Etest® method. The antimicrobial susceptibilities of bacterial isolates were determined using the agar diffusion method according to the CLSI. Screening for bla TEM, bla SHV, bla CTX-M genes and class 1 integron was performed by PCR amplification. To determine the genomic diversity of CTX-M-producers, isolates were analyzed by macrorestriction profile analysis following PFGE. Results and Discussion: Seventy-one K. pneumoniae isolates were ESBL-producing. PCR and sequencing experiments detected 38 CTX-M-producing K. pneumoniae belonged to groups CTX-M 1, CTX-M 2, CTX-M 8 and CTX-M 9. The association of different types ESBL (CTX-M, SHV and TEM) was frequent. All K. pneumoniae isolates carried class 1 integron. PFGE analysis revealed thirty-one clonal types among CTX-M-producing isolates. The data presented herein illustrate the diversity of genotypes of CTX-M producing K. pneumoniae among Brazilians hospitals.

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Section: Discussionmentioning

confidence: 99%

Diversity of genotypes in CTX-M-producing Klebsiella pneumoniae isolated in different hospitals in Brazil

Chagas¹,

Alves²,

Vallim³

et al. 2011

Braz J Infect Dis

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“…Presence of class 1 integrons was evaluated using primers 5'CS-3'CS (14), and mapping of gene cassettes harbored in class 1 integrons was performed as previously described (16).…”

Section: Detection and Sequencing Of Class 1 Integronsmentioning

confidence: 99%

Characterization of class 1 integrons and antibiotic resistance genes in multidrug-resistant Salmonella enterica isolates from foodstuff and related sources

Ribeiro¹,

Lincopán²,

Landgraf³

et al. 2011

Braz. J. Microbiol.

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In recent years, an increase in the occurrence of antimicrobial resistance among Salmonella enterica has been observed in several countries, which is worrisome because S. enterica is one of the most common causes of human gastroenteritis worldwide. The aim of this study was to characterize class 1 integrons and antibiotic resistance genotypes in Salmonella enterica isolates recovered from foodstuff and related sources.Nineteen multidrug-resistant (MDR) Salmonella enterica isolates were recovered. Higher resistance rates to tetracycline (90%), streptomycin (80%), sulfamethoxazole-trimethoprim (80%), ampicillin (60%) and nalidixic acid (70%) were related to the presence of the tetA, aadA, sul1/sul2, bla TEM-1 genes, and a codon mutation at position 83 of the gyrA gene, respectively. Class 1 integrons harboring aadA, bla TEM-1 , sul1 or dhfr1 genes were detected in nine (45%) Salmonella enterica strains belonging to serotypes Brandenburg, Panama, Agona, Mbandaka and Alachua. Finally, clonal dissemination of S. Panama, S. Derby and S.Mbandaka was confirmed by PFGE. Detection of clonally related MDR Salmonella enterica suggests that endemic serotypes can be supported by class 1 integron-borne gene cassettes and/or mutations in drug targets. Emergence and dissemination of multidrug-resistant Salmonella enterica can have a major public health impact in an environment where large-scale suppliers ship their products.

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“…1 The increasing resistance of Klebsiella pneumoniae to antibiotics has been a cause for worry since the 1980s after the emergence of extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae, which is resistant to all cephalosporins, 2 causing outbreaks in neonatal intensive care units. [3][4][5] This situation has become even more alarming in Brazil after the identification of Klebsiella pneumoniae with concomitant expression of IMP-1 metallobeta-lactamase and of ESBL of the CTX-M type, 6 as the joint production of these enzymes rendered the bacterium resistant to all commercially available antibiotics, including carbapenems.…”

Section: Introductionmentioning

confidence: 99%

Surto em berçário por Klebsiella pneumoniae produtora de beta-lactamase de espectro estendido atribuído à colonização de profissional de saúde portador de onicomicose

Cassettari¹,

Silveira²,

Balsamo³

et al. 2006

J. Pediatr. (Rio J.)

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First Isolation of Metallo-β-Lactamase-Producing Multiresistant Klebsiella pneumoniae from a Patient in Brazil

Cited by 87 publications

References 22 publications

Diversity of genotypes in CTX-M-producing Klebsiella pneumoniae isolated in different hospitals in Brazil

Diversity of genotypes in CTX-M-producing Klebsiella pneumoniae isolated in different hospitals in Brazil

Characterization of class 1 integrons and antibiotic resistance genes in multidrug-resistant Salmonella enterica isolates from foodstuff and related sources

Surto em berçário por Klebsiella pneumoniae produtora de beta-lactamase de espectro estendido atribuído à colonização de profissional de saúde portador de onicomicose

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