First Molecular Characterization of Bovine Leukemia Virus Infections in the Caribbean

Yang, Yi; Kelly, Patrick J.; Bai, Jianfa; Zhang, Rong; Wang, Chengming

doi:10.1371/journal.pone.0168379

Cited by 16 publications

(11 citation statements)

References 36 publications

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“…In Australia, only genotype-1 was detected [ 90 ]. In North America, genotypes −1, −3, and −4 have been detected in the USA [ 86 , 143 , 145 ], and genotype-1 was reported in the Caribbean [ 146 ]. In Central America, genotypes −1 and −5 were detected in Costa Rica [ 143 ].…”

Section: Introductionmentioning

confidence: 99%

Epidemiology and genetic diversity of bovine leukemia virus

Polat

Takeshima

Aida

2017

Virol J

152

154

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Bovine leukemia virus (BLV), an oncogenic member of the Deltaretrovirus genus, is closely related to human T-cell leukemia virus (HTLV-I and II). BLV infects cattle worldwide and causes important economic losses. In this review, we provide a summary of available information about commonly used diagnostic approaches for the detection of BLV infection, including both serological and viral genome-based methods. We also outline genotyping methods used for the phylogenetic analysis of BLV, including PCR restriction length polymorphism and modern DNA sequencing-based methods. In addition, detailed epidemiological information on the prevalence of BLV in cattle worldwide is presented. Finally, we summarize the various BLV genotypes identified by the phylogenetic analyses of the whole genome and env gp51 sequences of BLV strains in different countries and discuss the distribution of BLV genotypes worldwide.

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Section: Introductionmentioning

confidence: 99%

Epidemiology and genetic diversity of bovine leukemia virus

Polat

Takeshima

Aida

2017

Virol J

152

154

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“…Strains identified in our study from China are identified with filled circles (•). Genotypes shown on the right are according to Yang et al (2016b). The numbers at the branches show bootstrap support (1,000 replicates).…”

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confidence: 99%

“…previous study (Yang et al, 2016b). Amplicons were gel purified with the QIAquick Gel Extraction Kit and sequenced at the Genomic Sequencing Laboratory (GBI, Shanghai, China).…”

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confidence: 99%

Short communication: Genotyping and single nucleotide polymorphism analysis of bovine leukemia virus in Chinese dairy cattle

Yang

Chu

Shang

et al. 2019

Journal of Dairy Science

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Bovine leukemia virus (BLV) causes enzootic leucosis in cattle and is classified into 10 genotypes with a worldwide distribution, except for several European countries, Australia, and New Zealand. Although BLV is widespread in Chinese cows with the positive rate of 49.1% at the individual level, very little is known about the BLV genotype in dairy cattle in China. To determine BLV genetic variability in cows in China, 112 BLV-positive samples from 5 cities in China were used for BLV molecular characterization in this study. Phylogenetic analysis using the neighbor-joining method on partial env sequence encoding gp51 obtained from 5 Chinese cities and those available in GenBank (n = 53, representing BLV genotype 1-10) revealed the Chinese strains belonged to genotype 6. Seven unique SNP were identified among Yancheng, Shanghai, and Bengbu strains out of the total 12 SNP identified in Chinese strains. The genotyping coupled with SNP analysis of BLV can serve as a useful molecular epidemiological tool for tracing the source of pathogens. This study highlights the importance of genetic analysis of geographically diverse BLV strains to understand BLV global genetic diversity.

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“…For example, a predicted RFLP pattern of BLV env sequence of AY185360 using three endonucleases, Bcl I, Hae III, Pvu II [8], indicated that this BLV sequence was identified as Genotype 1. On the other hand, phylogenetic analysis in this study and other previous studies demonstrated that this sequence fell in cluster of Genotype 6 [7, 16, 19]. In spite of these misidentifications, identifications of new genotypes such as Genotype 9 in Bolivia and Genotype 10 in Thailand and Myanmar [7, 16] led to the development of a modified, broadly applicable PCR-RFLP method.…”

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confidence: 48%

Broadly applicable PCR restriction fragment length polymorphism method for genotyping bovine leukemia virus

Nishikaku

Ishikura

Ohnuki

et al. 2019

The Journal of Veterinary Medical Science

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Bovine leukemia virus (BLV) is a causative agent of enzootic bovine lymphoma (EBL). BLV is prevalent worldwide, and ten genotypes have been classified based on the sequence of the envelope glycoprotein (gp51) gene. In this study, we present a simple and generally applicable PCR restriction fragment length polymorphism (PCR-RFLP) method to identify BLV genotypes. While the genotyping results obtained by previously described PCR-RFLP methods matched only 78.96% to the results of phylogenetic analysis, we demonstrated that our PCR-RFLP method can identify 90.4% of the sequences available in the database in silico . The method was validated with 20 BLV sequences from EBL tumor tissues and 3 BLV sequences from blood of BLV infected cattle, and was found to show high specificity. We utilized this method to determine genotypes of blood samples from 18 BLV seropositive cattle in Kanagawa and Niigata, as well as 12 EBL cattle in Chiba, Japan. Our analysis with the modified PCR-RFLP detected two genotypes, Genotypes 1 and 3. Genotype 1 was detected as the main genotype, while Genotype 3 was sporadically observed. This technique can be used as a reliable system for screening a large number of epidemiological samples.

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First Molecular Characterization of Bovine Leukemia Virus Infections in the Caribbean

Cited by 16 publications

References 36 publications

Epidemiology and genetic diversity of bovine leukemia virus

Epidemiology and genetic diversity of bovine leukemia virus

Short communication: Genotyping and single nucleotide polymorphism analysis of bovine leukemia virus in Chinese dairy cattle

Broadly applicable PCR restriction fragment length polymorphism method for genotyping bovine leukemia virus

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