First proteome study of sporadic flowering in bamboo species (Bambusa vulgaris and Dendrocalamus manipureanus) reveal the boom is associated with stress and mobile genetic elements

Louis, Bengyella; Waikhom, Sayanika Devi; Goyari, Sailendra; Jose, Robinson C.; Roy, Pranab; Talukdar, Narayan Chandra

doi:10.1016/j.gene.2015.08.010

Cited by 7 publications

(2 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In turn, Wang et al (2008) used LC-MS/MS to study proteins activated by the moss Physcomitrella patens upon high salinity stress, revealing TE-derived proteins as being differentially expressed. Matrix Assisted Laser Desorption Ionization -Time of Flight (MALDI-TOF-TOF) combined with MS was also used to reveal proteomic background of sporadic flowering in bamboo species, suggesting a direct relationship of TE activation and the induction of flowering (Louis et al, 2015).…”

Section: Detection Of Te-encoded Proteinsmentioning

confidence: 99%

A review of strategies used to identify transposition events in plant genomes

et al. 2022

View full text Add to dashboard Cite

Transposable elements (TEs) were initially considered redundant and dubbed ‘junk DNA’. However, more recently they were recognized as an essential element of genome plasticity. In nature, they frequently become active upon exposition of the host to stress conditions. Even though most transposition events are neutral or even deleterious, occasionally they may happen to be beneficial, resulting in genetic novelty providing better fitness to the host. Hence, TE mobilization may promote adaptability and, in the long run, act as a significant evolutionary force. There are many examples of TE insertions resulting in increased tolerance to stresses or in novel features of crops which are appealing to the consumer. Possibly, TE-driven de novo variability could be utilized for crop improvement. However, in order to systematically study the mechanisms of TE/host interactions, it is necessary to have suitable tools to globally monitor any ongoing TE mobilization. With the development of novel potent technologies, new high-throughput strategies for studying TE dynamics are emerging. Here, we present currently available methods applied to monitor the activity of TEs in plants. We divide them on the basis of their operational principles, the position of target molecules in the process of transposition and their ability to capture real cases of actively transposing elements. Their possible theoretical and practical drawbacks are also discussed. Finally, conceivable strategies and combinations of methods resulting in an improved performance are proposed.

show abstract

Section: Detection Of Te-encoded Proteinsmentioning

confidence: 99%

A review of strategies used to identify transposition events in plant genomes

et al. 2022

View full text Add to dashboard Cite

show abstract

“…As well, P. edulis and D. latiflorus contain novel miRNAs playing important roles in regulating bamboo flowering ( Gao et al, 2015 ; Zhao et al, 2015 ). In addition, Louis et al (2015) used proteomics to find that elements of stress, mobile genetics, and signal transduction cross-talk were associated with sporadic flowering of bamboo. Undoubtedly, these results provide the basis for understanding the roles of genes involved in bamboo flowering but need further experimental evidence.…”

Section: Introductionmentioning

confidence: 99%

Overexpression of PvPin1, a Bamboo Homolog of PIN1-Type Parvulin 1, Delays Flowering Time in Transgenic Arabidopsis and Rice

Zhang

Yang

et al. 2017

Front. Plant Sci.

View full text Add to dashboard Cite

Because of the long and unpredictable flowering period in bamboo, the molecular mechanism of bamboo flowering is unclear. Recent study showed that Arabidopsis PIN1-type parvulin 1 (Pin1At) is an important floral activator and regulates floral transition by facilitating the cis/trans isomerization of the phosphorylated Ser/Thr residues preceding proline motifs in suppressor of overexpression of CO 1 (SOC1) and agamous-like 24 (AGL24). Whether bamboo has a Pin1 homolog and whether it works in bamboo flowering are still unknown. In this study, we cloned PvPin1, a homolog of Pin1At, from Phyllostachys violascens (Bambusoideae). Bioinformatics analysis showed that PvPin1 is closely related to Pin1-like proteins in monocots. PvPin1 was widely expressed in all tested bamboo tissues, with the highest expression in young leaf and lowest in floral bud. Moreover, PvPin1 expression was high in leaves before bamboo flowering then declined during flower development. Overexpression of PvPin1 significantly delayed flowering time by downregulating SOC1 and AGL24 expression in Arabidopsis under greenhouse conditions and conferred a significantly late flowering phenotype by upregulating OsMADS56 in rice under field conditions. PvPin1 showed subcellular localization in both the nucleus and cytolemma. The 1500-bp sequence of the PvPin1 promoter was cloned, and cis-acting element prediction showed that ABRE and TGACG-motif elements, which responded to abscisic acid (ABA) and methyl jasmonate (MeJA), respectively, were characteristic of P. violascens in comparison with Arabidopsis. On promoter activity analysis, exogenous ABA and MeJA could significantly inhibit PvPin1 expression. These findings suggested that PvPin1 may be a repressor in flowering, and its delay of flowering time could be regulated by ABA and MeJA in bamboo.

show abstract