First Report of Bacterial Leaf Blight of Rice Caused by <i>Xanthomonas oryzae</i> pv. <i>oryzae</i> in Benin

Afolabi, O.; Amoussa, R.; Bilé, M.; Oludare, Aderonke; Gbogbo, V.; Poulin, Lucie; Koebnik, Ralf; Szurek, Boris; Silué, D.

doi:10.1094/pdis-07-15-0821-pdn

Cited by 12 publications

(13 citation statements)

References 5 publications

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“…Signal of the causative agent as bacterial exudation was noticed on the boundaries and veins of newly infected leaf. The identical symptoms responsible for BLB of rice in West Bangal, India and also in Benin were also reported (Samanta et al, 2014, Afolabi et al, 2016. In the present study, sign of the causal agent as bacterial ooze was also noted on the veins or boundaries of the newly infected leaf under moist or humid conditions.…”

Section: Resultssupporting

confidence: 88%

Investigation of bacterial leaf blight of rice through various detection tools and its impact on crop yield in Punjab, Pakistan

Shaheen¹,

Shrif²,

Amrao³

et al. 2018

PAK.J.BOT.

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Bacterial blight caused by Xanthomonas oryzae pv. oryzae (Xoo) is an important bacterial disease in rice leading to heavy yield and economic losses. In current investigation, the rice leaf samples from infested regions were screened for Xoo strains linked to incidence of this disease. Subsequently, 17 different isolates were identified based on Gram staining, KOH assay and PCR analysis. Moreover, the percentage diseases incidence and weight loss of 1000-grains of each sample from four zones of rice production were recorded. Both biochemical test exhibited red stained and rod like Gram-ve bacteria. In addition, the molecular recognition by means of a 16S rRNA universal primer revealed DNA amplification in 15 out of 17 isolates which confirmed the pathogen as "Xoo". The data assessed for disease incidence in all investigated districts ranged between 70.12%-49.23%. While, the maximum and minimum weight losses of 17.84% and 11.17% from Sialkot and Narowal were recorded respectively. The application of such tools for Xoo detection and its impact on crop yield are contested in this investigation.

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Section: Resultssupporting

confidence: 88%

Investigation of bacterial leaf blight of rice through various detection tools and its impact on crop yield in Punjab, Pakistan

Shaheen¹,

Shrif²,

Amrao³

et al. 2018

PAK.J.BOT.

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“…The first observations of BB in Africa were made in Mali in 1979 (Buddenhagen et al, 1979). Since then, the disease has been reported mostly in West Africa, including Senegal, Benin, Burkina Faso, Ivory Coast, Mali and Niger (Afolabi et al, 2016; Gonzalez et al, 2007; Tall et al, 2020; Tekete et al, 2020). More recently, BB was also reported in the East African countries Uganda and Tanzania (Duku et al, 2016; Oliva et al, 2019).…”

Section: Resultsmentioning

confidence: 99%

“…More recently, BB was also reported in the East African countries Uganda and Tanzania (Duku et al, 2016; Oliva et al, 2019). To systematically analyze these newly isolated strains and to compare them to the broader African Xoo landscape, 833 strains from rice fields sampled in nine African countries between 2003 and 2021 were collected and characterized using molecular diagnostic as well as pathogenicity assays (Supplementary Table 1)(Afolabi et al, 2016; Gonzalez et al, 2007; Tall et al, 2020; Tekete et al, 2020). During this study, two unprecedented outbreaks were identified, one in 2019 in Dakawa and another in 2022 in Lukenge (Supplementary Table 2).…”

Section: Resultsmentioning

confidence: 99%

Genome editing of an African elite rice variety confers resistance against endemic and emergingXanthomonas oryzaepv.oryzaestrains

Schepler-Luu

Sciallano

Stiebner

et al. 2022

Preprint

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Bacterial leaf blight (BB) of rice, caused by Xanthomonas oryzae pv. oryzae (Xoo), threatens global food security and the livelihood of small-scale rice producers. Analyses of Xoo collections from Asia, Africa and the Americas demonstrated surprising continental segregation, despite robust global rice trade. Here, we report unprecedented BB outbreaks in Tanzania. The causative strains, unlike endemic Xoo, carry Asian-type TAL effectors targeting the sucrose transporter SWEET11a and suppressing Xa1. Phylogenomics clustered these strains with Xoo strains from China. African rice varieties do not carry suitable resistance genes. To protect African rice production against this emerging threat, we developed a hybrid CRISPR-Cas9/Cpf1 system to edit six TALe-binding elements in three SWEET promoters of the East African elite variety Komboka. The edited lines show broad-spectrum resistance against Asian and African strains of Xoo, including strains recently discovered in Tanzania. This strategy could help to protect global rice crops from BB pandemics.

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“…Sample collection: the flower samples used in this experiment were collected from the garden at International Institute of Tropical Agriculture (IITA), Cotonou, Benin. The bacteria pathogens were provided and earlier characterized by the Pathology Unit of Africa Rice, Cotonou (Lee, Hong, & Kim, 2010;Afolabi et al, 2016;Kini et al, 2017).…”

Section: Methodsmentioning

confidence: 99%

“…They are rodshaped, gram-negative bacteria found in both temperate and tropical parts of the world and cause 10-50% yield losses. Recently, Pantoea agglomerans were discovered to be the plight of farmers on wild rice Oryza longistaminata plants near Tanguiéta town, at Pendjari National Park, northwest Benin republic (Afolabi et al, 2016;Kini et al, 2017).…”

Section: Comparison Of the Extracts On Gram-positivementioning

confidence: 99%

Halochromic properties and antimicrobial potential of crude extracts from five species of ornamental plants

Kusimo

Ukoha

Oludare

et al. 2019

URJ

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Introduction: the colours of flowers are a result of secondary metabolites that have long been used in the medical and textile industries, and those that are halochromic are used in colour display because they change color according to pH changes, but many species are yet to be studied in detail. Objective: to explore the halochromic properties and the antimicrobial potentials of the crude extracts of several ornamental plants. Methods: we used aqueous and organic solvents to extract pigments from petals of five fascinating flowers planted around International Institute of Tropical Agriculture station, Cotonou, Benin: Allamanda blanchetii, Cascabela thevetia, Eichhornia crassipes, Ixora casei and Thunbergia erecta, followed by an investigation into their halochromic properties. Antibacterial potentials of the extracts were tested on important rice pathogens: Xanthomonas oryzae pv. oryzae, and Pantoea agglomerans, which are gram-negative bacteria; and on Bacillus subtilis, a gram-positive bacterium. Results: The crude extracts of T. erecta and A. blanchetii have good halochromic properties within pH 2 – 12, exhibiting distinct colours. The chromophores of the C. thevetia, E. crassipes, and I. casei are not halochromic as the colours of the crude extracts remain the same at the pH range except pH 12 which is similar for the five extracts. Crude extracts of T. erecta inhibited growth of P. agglomerans without development of resistance, whereas the bacteria developed resistance against Penicillin after 18 hrs of incubation. T. erecta and A. blanchetii were able to inhibit growth of X. oryzae and both inhibited B. subtilis. Conclusion: Pigments from both T. erecta and A. blanchetii are good pH indicators; however, T. erecta is a better antibacterial agent than A. blanchetii because it has broad-spectrum activities against bacteria.

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First Report of Bacterial Leaf Blight of Rice Caused by Xanthomonas oryzae pv. oryzae in Benin

Cited by 12 publications

References 5 publications

Investigation of bacterial leaf blight of rice through various detection tools and its impact on crop yield in Punjab, Pakistan

Investigation of bacterial leaf blight of rice through various detection tools and its impact on crop yield in Punjab, Pakistan

Genome editing of an African elite rice variety confers resistance against endemic and emergingXanthomonas oryzaepv.oryzaestrains

Halochromic properties and antimicrobial potential of crude extracts from five species of ornamental plants

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