First Report of <i>Sugarcane yellow leaf virus</i> Infecting Grain Sorghum (<i>Sorghum bicolor</i>) in the United States

Wei, Chunyan; Hincapie, Martha; Larsen, Nicholas A.; Nuessly, Gregg S.; Rott, Philippe

doi:10.1094/pdis-02-16-0183-pdn

Cited by 19 publications

(11 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results corroborate findings by Wei et al . () and Espinoza Delgado et al . () who reported infection of grain sorghum and Columbus grass, respectively, by SCYLV transmitted by M. sacchari .…”

Section: Discussionmentioning

confidence: 90%

Phylogenetic and recombination analysis of sorghum isolates of Sugarcane yellow leaf virus

et al. 2017

View full text Add to dashboard Cite

Recombination has played an important role in evolution and genetic diversity of Sugarcane yellow leaf virus (SCYLV) isolates sequenced to date. This study found that three newly sequenced SCYLV sorghum isolates from the USA underwent intraspecies recombination. No statistical significance on probable progeny-parent relationships involving SCYLV sorghum isolates were found in possible interspecies recombination with 18 members of the Luteoviridae family. Sorghum isolates deposited in the GenBank database under accession numbers KT960995, KT960996 and KT960997 were phylogenetically closely related to SCYLV genotypes IND, CUB and CHN1, all members of phylogroup II. Networked relationships among the sorghum isolates showed that numerous incompatibilities occurred in the sequences. These conflicting signals were probably due to recombination, especially in KT960997, which was heavily impacted by recombination. The KT960997 accession was positioned on a distinct branch compared to other members of phylogroup II, suggesting that it has probably emerged as a new genotype. Future studies on molecular evolution may reveal further insights into the adaptation capacity of these SCYLV lineages to new environments.

show abstract

Section: Discussionmentioning

confidence: 90%

Phylogenetic and recombination analysis of sorghum isolates of Sugarcane yellow leaf virus

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Top76-6 by Elsayed et al (2018) based on the NCBI GenBank accession numbers (KT960997, KT960996, and KT960995). Similarly, from the United States the natural occurrence of SCYLV on S. bicolor and Columbus grass (Sorghum almum) was identified (Espinoza-Delgado et al, 2016; Wei et al, 2016).…”

Section: Recognition Of Physical Properties Symptomatology and Diagmentioning

confidence: 99%

Present Status and Future Management Strategies for Sugarcane Yellow Leaf Virus: A Major Constraint to the Global Sugarcane Production

et al. 2020

View full text Add to dashboard Cite

Sugarcane yellow leaf virus (SCYLV) is a distinct member of the Polerovirus genus of the Luteoviridae family. SCYLV is the major limitation to sugarcane production worldwide and presently occurring in most of the sugarcane growing countries. SCYLV having high genetic diversity within the species and presently ten genotypes are known to occur based on the complete genome sequence information. SCYLV is present in almost all the states of India where sugarcane is grown. Virion comprises of 180 coat protein units and are 24-29 nm in diameter. The genome of SCYLV is a monopartite and comprised of single-stranded (ss) positive-sense (+) linear RNA of about 6 kb in size. Virus genome consists of six open reading frames (ORFs) that are expressed by sub-genomic RNAs. The SCYLV is phloem-limited and transmitted by sugarcane aphid Melanaphis sacchari in a circulative and non-propagative manner. The other aphid species namely, Ceratovacuna lanigera, Rhopalosiphum rufiabdominalis, and R. maidis also been reported to transmit the virus. The virus is not transmitted mechanically, therefore, its transmission by M. sacchari has been studied in different countries. SCYLV has a limited natural host range and mainly infect sugarcane (Sachharum hybrid), grain sorghum (Sorghum bicolor), and Columbus grass (Sorghum almum). Recent insights in the protein-protein interactions of Polerovirus through protein interaction reporter (PIR) technology enable us to understand viral encoded proteins during virus replication, assembly, plant defence mechanism, short and long-distance travel of the virus. This review presents the recent understandings on virus biology, diagnosis, genetic diversity, virus-vector and host-virus interactions and conventional and next generation management approaches.

show abstract

“…On the other hand, this latter study was conducted 20 years ago, and several aspects of disease epidemiology have changed PLOS ONE since then. Sugarcane cultivars grown in the EAA in the 1990s are no longer grown commercially and new natural hosts of SCYLV have recently been identified [34,35]. Furthermore, in 2013, massive outbreaks of M. sacchari occurred on sorghum in the USA [43].…”

Section: Plos Onementioning

confidence: 99%

“…SCYLV may have not been found in aphids raised on Columbus grass because the virus titer was low in this plant species or because the aphid haplotype that was used for these transmission experiments originated from sugarcane and was not adapted for transmission to sorghum species. Previous studies showed that the virus can be detected in M. sacchari (most likely haplotype H1) collected from SCYLV-infected plants of S. bicolor in Florida [35].…”

Section: Plos Onementioning

confidence: 99%

See 1 more Smart Citation

Lack of transmission of Sugarcane yellow leaf virus in Florida from Columbus grass and sugarcane to sugarcane with aphids or mites

et al. 2020

View full text Add to dashboard Cite

Sugarcane yellow leaf virus (SCYLV), the causal agent of yellow leaf disease, naturally infects at least three plant species in Florida: sugarcane (Saccharum spp.), the weed Columbus grass (Sorghum almum) and cultivated sorghum (S. bicolor). All three hosts are also colonized by the sugarcane aphid (Melanaphis sacchari), the main vector of SCYLV worldwide. To understand the high incidence of SCYLV observed in sugarcane commercial fields and in germplasm collections, we investigated the transmission efficiency of SCYLV from sugarcane and Columbus grass to sugarcane using the sugarcane aphid and a spider mite (Oligonychus grypus) that also tested positive for SCYLV in Florida. Healthy and SCYLV-infected leaf pieces of sugarcane and Columbus grass carrying viruliferous aphids or spider mites were transferred to virus-free plants of the yellow leaf susceptible sugarcane cultivar CP96-1252. Three-and 6-months post inoculation, the 108 aphid-inoculated plants of Columbus grass and the 90 mite-inoculated plants of sugarcane tested negative for SCYLV by tissue blot immunoassay (TBIA) or reverse transcription polymerase chain reaction (RT-PCR). Similar results were obtained for 162 aphid-inoculated plants of sugarcane, except for two plants that tested positive for SCYLV by TBIA and RT-PCR. In two field experiments planted with SCYLV-free and virus-infected sugarcane (cultivar CP96-1252), only 18-28% of healthy plants became infected during a 24-to 28-month period. SCYLV prevalence in these field experiments did not differ between aphicide treated and untreated plots. Incidence of M. sacchari haplotypes in the Everglades agricultural area also indicated that the predominant haplotype that is currently colonizing sugarcane was not a vector of SCYLV in Florida. Lack of virus transmission by the spider mite suggested that this arthropod only acquired the virus when feeding on infected plants but was unable to transmit SCYLV. The current vector of SCYLV in Florida remains to be identified.

show abstract

First Report of Sugarcane yellow leaf virus Infecting Grain Sorghum (Sorghum bicolor) in the United States

Cited by 19 publications

References 2 publications

Phylogenetic and recombination analysis of sorghum isolates of Sugarcane yellow leaf virus

Phylogenetic and recombination analysis of sorghum isolates of Sugarcane yellow leaf virus

Present Status and Future Management Strategies for Sugarcane Yellow Leaf Virus: A Major Constraint to the Global Sugarcane Production

Lack of transmission of Sugarcane yellow leaf virus in Florida from Columbus grass and sugarcane to sugarcane with aphids or mites

Contact Info

Product

Resources

About