2021
DOI: 10.1002/ndr2.12020
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First report of Turnip yellows virus in cabbage in the Philippines

Abstract: Brassica oleracea var. capitata) showing leaf yellowing symptoms In March 2017, 52 dried-leaf samples of brassicaceous plants from the Philippine Highlands, one of which was a sample of cabbage (Brassica oleracea var. capitata; Fig. 1), were submitted to Fera Science Ltd., via the Centre for Agriculture and Bioscience International (CABI). The plants were all displaying virus-like, yellowing symptoms (Reeder et al., 2017).Initially, samples were bulked together and tested by ELISA for the presence of viruses k… Show more

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Cited by 6 publications
(3 citation statements)
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“…Finally, although not identified during the de novo assembly and mapping of the HTS reads of the five historical SCMoV isolates against previously identified satellites (Davies et al 1990), BWA (Li and Durbin 2009) and SAMtools ) revealed single satellite RNAs associated with each isolate (satellite accession numbers OM818397-OM818401) with between 96.4-98.2% nt identities to SCMoV satellite with accession number M33001.1 (Davies et al 1990). Presence of a turnip yellows virus (TuYV) sequence within samples containing isolates AL, MB and NN was confirmed by real time PCR (Buxton-Kirk et al 2021). By contrast, SS did not identify satellite RNA or TuYV within any samples presumably because SS employs virus-specific or group-specific degenerate primers, whereas HTS can potentially sequence any RNA present in a sample.…”
Section: Tablementioning
confidence: 96%
“…Finally, although not identified during the de novo assembly and mapping of the HTS reads of the five historical SCMoV isolates against previously identified satellites (Davies et al 1990), BWA (Li and Durbin 2009) and SAMtools ) revealed single satellite RNAs associated with each isolate (satellite accession numbers OM818397-OM818401) with between 96.4-98.2% nt identities to SCMoV satellite with accession number M33001.1 (Davies et al 1990). Presence of a turnip yellows virus (TuYV) sequence within samples containing isolates AL, MB and NN was confirmed by real time PCR (Buxton-Kirk et al 2021). By contrast, SS did not identify satellite RNA or TuYV within any samples presumably because SS employs virus-specific or group-specific degenerate primers, whereas HTS can potentially sequence any RNA present in a sample.…”
Section: Tablementioning
confidence: 96%
“…TuYV was confirmed by real‐time RT‐PCR (Buxton‐Kirk et al., 2021). This is the first host record of TuYV in Narcissus .…”
Section: Figurementioning
confidence: 99%
“…BrYV was first identified in China in 2011 ( 10 ) and is tentatively placed in the genus Polerovirus (Solemoviridae); it has since been reported in Korea ( 11 ), Japan ( 13 ) and Australia ( 14 ). Molecular and phylogenetic studies show BrYV is closely related to turnip yellows virus (TuYV: Luteoviridae) ( 10 , 14 , 15 ), which has been reported throughout Europe, South Africa, China, Iran, Egypt, South Africa, Egypt, Morocco, and Australia, and infects crops and weeds, including members of the Brassicaceae, Fabaceae, Amaranthaceae and Asteraceae (see references in [14]). Poleroviruses are mostly transmitted by aphids in a circulative and nonreplicative mode ( 16 19 ); one known polerovirus is transmitted by whiteflies, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) ( 20 ).…”
Section: Introductionmentioning
confidence: 99%