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Brassica oleracea var. capitata) showing leaf yellowing symptoms In March 2017, 52 dried-leaf samples of brassicaceous plants from the Philippine Highlands, one of which was a sample of cabbage (Brassica oleracea var. capitata; Fig. 1), were submitted to Fera Science Ltd., via the Centre for Agriculture and Bioscience International (CABI). The plants were all displaying virus-like, yellowing symptoms (Reeder et al., 2017).Initially, samples were bulked together and tested by ELISA for the presence of viruses known to occur in the Brassicaceae includingThis is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

The seeds were imported from Asia and samples were screened in compliance with import testing requirements. A total of 3,000 seeds were tested for Tomato mottle mosaic virus (ToMMV; genus Tobamovirus) using the RT-PCR test (F-5476 and R-6287) from Levitzky et al. (2019). A product of the expected size (811 bp) was obtained in all subsamples (12 subsamples each of 250 seeds) and subsequently sent for Sanger sequencing. Sequences from four of these samples were compared against those in GenBank, and confirmed to be ToMMV (Accession Nos. OK334226, OK334230-32), with ≥99% nucleotide and 99-100% amino acid identity compared with the exemplar strain (KF477193; YP_008492931.1). To obtain the whole genome of the isolate, the 12 subsamples were bulked and sequenced by high throughput sequencing (HTS) as described by Fox et al. (2019) and bioinformatic analysis using the Angua pipeline (Fowkes et al., 2021). The whole genome of ToMMV was obtained (6375 bp; OK334224), and confirmed after a BLAST search (99.6% identity KF477193.1; Figure 1).The genome of Tomato mosaic virus (ToMV) was also found (OK334225).

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