First report of pepper as a natural new host for<i>Tomato marchitez virus</i>in Sinaloa, Mexico

Camacho-Beltrán, Erika; Armenta-Chávez, Rogelio; Romero-Romero, Jesús L.; Magallanes-Tapia, Marco Antonio; Leyva-López, Norma Elena; Apodaca-Sánchez, Miguel Ángel; Méndez-Lozano, Jesús

doi:10.1080/07060661.2015.1078412

Cited by 5 publications

(5 citation statements)

References 29 publications

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“…The ToTV isolates used for the amplifications were as follows: three Polish isolates, Wal′03 (EU563947) [ 2 ], Kra (KJ940974), and Ros (KM114266) [ 26 ]); four Spanish isolates, ALM04 (GQ397437), ALC07 (GQ397442), MUR07 (GQ397443), and MUR05 (GQ397439) [ 8 ]; four recombinant infectious clones based on the Kra2014 ToTV RNA2 sequence [ 27 ]; the mutants ToTV-Kra sec1 and sec3 (the Vp35 amino acid sequences were derived from ToTV isolates sec1 [KJ571198] and sec3 [KJ571200], respectively); and ToTV-Kra-G759A (generated in our laboratory). Tomato marchitez virus (ToMarV) [ 28 ] and tomato apex necrosis virus (ToANV; EF063642) [ 29 ], which is believed to be a ToMarV strain, were used as negative controls. ToMarV has been isolated from tomatoes in Mexico and is closely related to ToTV according to phylogenetic analysis.…”

Section: Introductionmentioning

confidence: 99%

One-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) for detection of tomato torrado virus

Budziszewska

Wieczorek

Obrępalska‐Stęplowska

2016

Arch Virol

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‘Torrado’ disease caused by tomato torrado virus (ToTV) is responsible for considerable losses in tomato production. Therefore, a one-step reverse transcription loop-mediated isothermal amplification protocol for early and fast detection of ToTV isolates has been developed. The RNA extracted from ToTV-infected plants was tested using this protocol with a set of six primers specific for the Vp35 coat protein gene sequence. The amplified products were analyzed using amplification curves, electrophoresis, and direct staining of DNA. The sensitivity of the protocol was tenfold higher than that of conventional RT-PCR. This new protocol is inexpensive, rapid, simple, and very sensitive.

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Section: Introductionmentioning

confidence: 99%

One-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) for detection of tomato torrado virus

Budziszewska

Wieczorek

Obrępalska‐Stęplowska

2016

Arch Virol

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“…ToTV was also reported in tomato-growing areas in the Mediterranean basin (e.g., Spain and Italy), in Poland, Hungary, and Australia (Hanssen et al, 2010a;Hanssen and Lapidot, 2012) and recently in Serbia (Vučurović et al, 2021). On the contrary, since the discovery of ToMarV in tomatoes from Mexico showing severe leaf necrosis and necrotic rings on fruits in 2007 (Verbeek et al, 2008), natural infection of ToMarV was only reported in pepper in Mexico (Camacho-Beltrán et al, 2015).…”

Section: Secoviridae: Nepoviruses and Torradovirusesmentioning

confidence: 99%

Global Advances in Tomato Virome Research: Current Status and the Impact of High-Throughput Sequencing

et al. 2021

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Viruses cause a big fraction of economically important diseases in major crops, including tomato. In the past decade (2011–2020), many emerging or re-emerging tomato-infecting viruses were reported worldwide. In this period, 45 novel viral species were identified in tomato, 14 of which were discovered using high-throughput sequencing (HTS). In this review, we first discuss the role of HTS in these discoveries and its general impact on tomato virome research. We observed that the rate of tomato virus discovery is accelerating in the past few years due to the use of HTS. However, the extent of the post-discovery characterization of viruses is lagging behind and is greater for economically devastating viruses, such as the recently emerged tomato brown rugose fruit virus. Moreover, many known viruses still cause significant economic damages to tomato production. The review of databases and literature revealed at least 312 virus, satellite virus, or viroid species (in 22 families and 39 genera) associated with tomato, which is likely the highest number recorded for any plant. Among those, here, we summarize the current knowledge on the biology, global distribution, and epidemiology of the most important species. Increasing knowledge on tomato virome and employment of HTS to also study viromes of surrounding wild plants and environmental samples are bringing new insights into the understanding of epidemiology and ecology of tomato-infecting viruses and can, in the future, facilitate virus disease forecasting and prevention of virus disease outbreaks in tomato.

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“…The viral particles of ToMarV are retained inside the stylet of whitefly (Hemiptera: Aleyrodidae) and transmitted in a semi-persistent manner by B. tabaci, Trialeurodes vaporarorium, and T. abutilonea [99]. Furthermore, the virus can be mechanically transmitted in chili plants, but the transmission efficiency is much lower than in tomato [98]. In the case of transmission by seed, there is no information available for this virus.…”

Section: Tomato Marchitez Virusmentioning

confidence: 99%

“…Thus, the purified virus allowed the RNA extraction and subsequent genomic characterization. Once the ToMarV sequence was known, it allowed the design of assays based on RT-PCR for detection [98]. Additionally, Western and Northern blot tests were reported during the production of an infectious clone of ToMarV [97].…”

Section: Tomato Marchitez Virusmentioning

confidence: 99%

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Viruses of Economic Impact on Tomato Crops in Mexico: From Diagnosis to Management—A Review

García‐Estrada

Diaz‐Lara

Aguilar-Molina

et al. 2022

Viruses

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Tomato is the most economically important vegetable crop worldwide and the second most important for Mexico. However, viral diseases are among the main limiting factors that affect the productivity of this crop, causing total losses in some cases. This review provides key information and findings on the symptoms, distribution, transmission, detection, and management of diseases caused by viruses of major importance in tomato crops in Mexico. Currently, about 25 viruses belonging to nine different families have been reported infecting tomato in Mexico, but not all of them cause economically significant diseases. Viruses of economic importance include tomato brown rugose fruit virus (ToBRFV), tomato spotted wilt virus (TSWV), tomato yellow leaf curl virus (TYLCV), pepino mosaic virus (PepMV), and tomato marchitez virus (ToMarV). The topics discussed here will provide updated information about the status of these plant viruses in Mexico as well as diverse management strategies that can be implemented according to the specific circumstances of each viral pathosystem. Additionally, a list of tomato-affecting viruses not present in Mexico that are continuous threats to the crop health is included.

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First report of pepper as a natural new host forTomato marchitez virusin Sinaloa, Mexico

Cited by 5 publications

References 29 publications

One-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) for detection of tomato torrado virus

One-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) for detection of tomato torrado virus

Global Advances in Tomato Virome Research: Current Status and the Impact of High-Throughput Sequencing

Viruses of Economic Impact on Tomato Crops in Mexico: From Diagnosis to Management—A Review

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