Fixed-Bed Column Process as a Strategy for Separation and Purification of Cephamycin C from Fermented Broth

Abstract

Fixed-bed column processes using the anionic resin Q Sepharose XL were evaluated for cephamycin C (CepC) purification from fermentation broth. Breakthrough and desorption curves were obtained for different flow rates (2.5, 5.0, and 7.5 mL/min). The elution method consisted of a stepwise gradient using NaCl solutions (0.1%, 0.3%, and 0.5%), which resulted in the separation of CepC from other antibiotics. The flow rate did not interfere in adsorption during loading of the column, but band broadening was observed during elution as the flow rate was increased. After the ion-exchange process, the fractions containing CepC were subjected to solid-phase extraction using a C18 cartridge to remove salts. Analyses of the broth used to feed the column and of the salt-free fractions by LC−MS showed a reduction in the concentration of some contaminants (possibly penicillin N, deacetylcephalosporin C, and deacetoxycephalosporin C) compared to the concentration of CepC. In conclusion, ion exchange followed by adsorption on a C18 adsorbent was demonstrated to be a selective and efficient procedure for the purification of CepC from fermentation broth.

Hydrophobic Adsorption Followed by Desorption with Ethanol–Water for Recovery of Penicillin G from Fermentation Broth

“…The liquid extraction method has a number of drawbacks, including high product losses, toxicity, the risk of product contamination, and volatility/flammability of the solvent. Furthermore, the product should ideally be continuously removed from the fermentation process (extractive fermentation), as a way to improve productivity and yield; , although in practice, this is not done, largely due to the volumes of solvent required and regulation restrictions. Despite these problems, this method is still employed in the industry since it is cheap and efficient.…”

“…Hydrophobic adsorption is a relatively simple process that is extensively used industrially and in environmental cleanup procedures. ,, In this technique, desorption of the antibiotic may be performed with an environmentally friendly solvent. The process can be adapted and scaled-up for running in a cyclic batch column system, in parallel with the production of PenG, hence enabling simultaneous production and extraction.…”

Selective Recovery of Copper from a Synthetic Metalliferous Waste Stream Using the Thiourea-Functionalized Ion Exchange Resin Puromet MTS9140

“…While the ionic radii of Cu(I) (0.46-0.77 Å) is substantially smaller than that of ClO 3 − (1.71 Å), it is unlikely that Cu residing in pores inaccessible to ClO 3− was responsible for this observation given the flexibility of gelled polymers [18]. Instead, this is more likely a result of kinetic limitations inherent to column operation, i.e., the limited residence time within the column is hindering sufficient mass transfer between the bulk eluent and resin, and in doing so reduces eluent efficiency [35]. It is expected that such effects are amplified when coupled with functionality degradation on resin outer surfaces.…”