Flavobacterium jocheonensis sp. nov., Isolated from Marine Green Alga Ulva pertusa

Choi, H.; Heo, Moon Soo

doi:10.4014/jmb.1812.12011

“…The marine members of the bacteroidetal class Flavobacteria have been associated with the degradation of marine high molecular weight particulate organic matter, since the marine Flavobacterium strains are often enriched on detritus and colonize surfaces of living organisms, such as corals, algae and higher plants [16]. Recently, many Flavobacterium species such as Flavobacterium ajazii [4], Flavobacterium coralii [17], Flavobacterium jocheonensis [18] and Flavobacterium zhairuonensis [19] have been isolated from various marine samples, including seaweed, coral, marine green algae and sediment, respectively.…”

Section: Introductionmentioning

confidence: 99%

Flavobacterium litorale sp. nov., isolated from red alga

Muhammad

¹

,

Han

²

,

Lee

³

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

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A Gram-stain-negative and rod-shaped bacterial strain (WSW3-B6T) was isolated from red alga collected from the West Sea, Republic of Korea. Cells of strain WSW3-B6T were non-motile, aerobic and produced slightly yellow and mucoid colonies on marine agar. The strain grew optimally at 23–30 °C, with 0.5–4 % NaCl (w/v) and at pH 6.5–8.5. A phylogenetic analysis of the 16S rRNA gene revealed that strain WSW3-B6T belongs to the genus Flavobacterium within the family Flavobacteriaceae , having the highest sequence similarity to Flavobacterium arcticum SM1502T (96.7%), followed by Flavobacterium salilacus subsp. altitudinum LaA7.5T (96.2%) and Flavobacterium salilacus subsp. salilacus SaA2.12T (96.2%). The complete sequence of a circular chromosome of strain WSW3-B6T determined by combination of Oxford Nanopore and Illumina platforms comprised a total 2 725 095 bp with G+C content of 37.1 mol%. A comparative analysis based on the whole genome also showed the distinctiveness of strain WSW3-B6T. The average nucleotide identity (ANI) values between strain WSW3-B6T and the closest strains F. arcticum SM1502T, F. salilacus subsp. altitudinum LaA7.5T and F. salilacus subsp. salilacus SaA2.12T were 78.3, 77.8 and 77.7 %, respectively, while the digital DNA–DNA hybridization (dDDH) values between strain WSW3-B6T and the above closely related strains were 21.0, 20.4 and 20.3 %, respectively. Both the ANI and dDDH values supported the creation of a new species in the genus Flavobacterium . The major fatty acids (>10 %) were iso-C15 : 0 (19.3 %), C16 : 0 (14.0 %), iso-C17 : 0 3-OH (13.1 %) and C18 : 0 (10.7 %). The polar lipids of strain WSW3-B6T included phosphatidylethanolamine, three unidentified aminolipids and three unidentified lipids. Moreover, MK-6 was the only respiratory quinone. A comparison of the phylogenetic distinctiveness and the unique phenotypic and chemotaxonomic characteristics among strain WSW3-B6T and closely related type strains supported that strain WSW3-B6T (=KCTC 82708T=GDMCC 1.2627T) represents a novel species of the genus Flavobacterium , for which the name Flavobacterium litorale sp. nov. is proposed.

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“…sharifuzzamanii', 'F. tibetense' and F. niveum [6][7][8][9][10][11]. Flavobacterium species have been isolated from various habitats, i.e.…”

Section: Introductionmentioning

confidence: 99%

Flavobacterium alkalisoli sp. nov., isolated from rhizosphere soil of Suaeda salsa

Xu

¹

,

Wang

²

,

Zhang

³

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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A Gram-negative, strictly aerobic, gliding motility, none-spore forming, yellow, rods bacterial strain, designated XS-5T, was isolated from rhizosphere soil of Suaeda salsa, in Tumd Right Banner, Inner Mongolia, PR China. A phylogenetic tree based on the 16S rRNA gene sequences and the phylogenomic tree both showed that strain XS-5T clustered with Flavobacterium beibuense F44-8T (shared 97.2 % of 16S rRNA gene similarity) and Flavobacterium rakeshii FCS-5T (97.6 %), and shared <96.0 % of 16S rRNA gene similarities with all other type strains. Strain XS-5T contained MK-6 as the major respiratory quinone. Its major polar lipids were phosphatidylethanolamine, an unidentified aminolipid and an unidentified lipid; and the major fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH, C16 : 0, iso-C15 : 0 3-OH, Summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1 ω7c), and Summed feature 9 (iso-C17 : 1 ω9c and/or C16 : 0 10-methyl). The genome consisted of a 3 985 855 bp circular chromosome, with a G+C content of 37.9 mol%, predicting 3616 coding sequences genes, 45 tRNA genes and three rRNA operons. The average nucleotide identity, amino acid identity and digital DNA–DNA hybridization values of strain XS-5T to F. beibuense F44-8T and F. rakeshii FCS-5T were 79.2 and 79.2 %, 81.7 and 81.6 %, 22.3 and 22.2 %, respectively. The results of phylogenetic, physiological and biochemical tests allowed the discrimination of strain XS-5T from its phylogenetic relatives. Flavobacterium alkalisoli sp. nov. is therefore proposed with strain XS-5T (=CGMCC 1.17077T=KCTC 72459T) as the type strain.

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“…The species of this genus are characterized by the presence of phosphatidylethanolamine, menaquinone-6 (MK-6) and iso-C 15 : 0 alongside summed feature 3 (iso-C 15 : 0 2-OH and/or C 16 : 1 ω7c) as the principal polar lipid, predominant respiratory quinone and common major fatty acids, respectively, and DNA G+C contents of 29.9-48.9 mol%. They have been isolated from various habitats, such as glacier ice, seawater, freshwater and plant rhizospheres [4][5][6][7][8], as well as algal [9,10] or cyanobacterial cultures [11,12]. Results from many studies have revealed that species of the genus Flavobacterium become abundant during cyanobacterial blooms, particularly within cyanobacteria aggregates [13,14].…”

Section: Introductionmentioning

confidence: 99%

Flavobacterium phycosphaerae sp. nov. isolated from the phycosphere of Microcystis aeruginosa

Kim

¹

,

Lee

²

,

Lee

³

et al. 2019

International Journal of Systematic and Evolutionary Microbiology

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A novel aerobic bacterial strain, designated MK012T, was isolated from the phycosphere of Microcystis aeruginosa . MK012T appears to be Gram-stain-negative, non-motile and rod-shaped and has yellow pigments. Phylogenetic analysis using 16S rRNA revealed that this bacterium was most closely related to Flavobacterium buctense T7T (=JCM 30750T; 97.5 %), Flavobacterium dankookense ARSA-19T (=KCTC 23179T; 97.5 %) and Flavobacterium macrobrachii an-8T (=DSM 22219T; 97.3 %). The genome size and genomic DNA G+C content of MK012T were estimated at 3.3 Mbp and 37.6 mol%, respectively. The average nucleotide identity (ANI), average amino acid identity (AAI) and in silico DNA–DNA hybridization (dDDH) values of MK012T and the members of the genus Flavobacterium were found to be 71.8–78.3 %, 65.1–79.6% and 19.1–21.5 %, respectively. MK012T exhibited oxidase but no catalase activity. The cells grew at 14–36 °C (optimum, 25 °C), pH 5–10 (optimum, pH 7) and 0–0.4 % (w/v) NaCl (optimum, 0 % NaCl) in R2A medium. MK012T did not produce flexirubin-type pigments. The predominant cellular fatty acids of MK012T were determined to be iso-C15 : 0, iso-C15 : 0 3-OH, iso-C17 : 0 3-OH, and summed feature 9 (10-methyl C16 : 0 and/or iso-C17 : 1ω9c); MK-6 was identified as its only respiratory quinone. Its major polar lipids were determined to be phosphatidylethanolamine, two unidentified aminolipids, and two unidentified polar lipids. The genotypic and phenotypic characteristics indicate that MK012T (=KACC 21509T=JCM 33879T) represents a novel species of the genus Flavobacterium ; therefore, the name Flavobacterium phycosphaerae sp. nov. is proposed.

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Flavobacterium jocheonensis sp. nov., Isolated from Marine Green Alga Ulva pertusa

Cited by 3 publications

References 31 publications

Flavobacterium litorale sp. nov., isolated from red alga

Flavobacterium litorale sp. nov., isolated from red alga

Flavobacterium alkalisoli sp. nov., isolated from rhizosphere soil of Suaeda salsa

Flavobacterium phycosphaerae sp. nov. isolated from the phycosphere of Microcystis aeruginosa

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