Flavobacterium rivuli sp. nov., Flavobacterium subsaxonicum sp. nov., Flavobacterium swingsii sp. nov. and Flavobacterium reichenbachii sp. nov., isolated from a hard water rivulet

Ali, Zahid; Cousin, Sylvie; Frühling, Anja; Brambilla, Evelyne; Schümann, Peter; Yang, Yun; Stackebrandt, Erko

doi:10.1099/ijs.0.008771-0

Cited by 59 publications

(43 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Menaquinones were extracted with chloroform/methanol (2 : 1, v/v) and analysed using HPLC as described by Komagata & Suzuki (1987). The G+C content of the genomic DNA was determined to be 34.2 mol%, within the range reported for the genus Flavobacterium (30-52 mol%; Ali et al, 2009;Bernardet & Bowman, 2011;Liu et al, 2010). The cellular fatty acids of strain G16-7 T and its relatives are listed in Table 1.…”

Section: Flavobacterium Dongtanense Lw30 T (Gu073293)mentioning

confidence: 99%

“…Values are percentages of the total fatty acids present. Data for strains 1-6 were obtained from the present study; data for strains 7-9 were from Kuo et al (2013) and Ali et al (2009) an unknown aminophospholipid, four unknown aminolipids and three unknown lipids. Strain G16-7 T exhibited a similar polar lipid profile to its closest relatives, F. rakeshii FCS-5 T , F. suzhouense XIN-1 T and F. beibuense F44-8 T ; they all had phosphatidylethanolamine and several unknown lipids and aminolipids as the major polar lipids.…”

Section: Flavobacterium Dongtanense Lw30 T (Gu073293)mentioning

confidence: 99%

“…NaCl tolerance was tested using modified LB broth (without NaCl) supplemented with 0-9 % (w/v) NaCl (at 1 % intervals). Antibiotic sensitivity tests were performed using the diffusion method (Fraser & Jorgensen, 1997) on LB agar and MuellerHinton agar with filter-paper discs (8 mm diameter) containing one of the following antibiotics: ampicillin (10 mg), amoxicillin (10 mg), bacitracin (0.04 U), carbenicillin (100 mg), cefradine (30 mg), chloromycetin (30 mg), erythromycin (15 mg), gentamicin (10 mg), kanamycin (30 mg), lincomycin (2 mg), novobiocin (30 mg), penicillin G (10 U), polymyxin (300 I), rifampicin (5 mg), roxithromycin (15 mg), spectinomycin (100 mg), streptomycin (Ali et al, 2009). Data for columns 1-6 marked with an asterisk were taken from Zhang et al (2015a), Dong et al (2013b), Fu et al (2011), Kaur et al (2012) and Yang et al (2015).…”

Section: Flavobacterium Dongtanense Lw30 T (Gu073293)mentioning

confidence: 99%

See 2 more Smart Citations

Flavobacterium suaedae sp. nov., an endophyte isolated from the root of Suaeda corniculata

Sun

Liu

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

Section: Flavobacterium Dongtanense Lw30 T (Gu073293)mentioning

confidence: 99%

Section: Flavobacterium Dongtanense Lw30 T (Gu073293)mentioning

confidence: 99%

Section: Flavobacterium Dongtanense Lw30 T (Gu073293)mentioning

confidence: 99%

See 1 more Smart Citation

Flavobacterium suaedae sp. nov., an endophyte isolated from the root of Suaeda corniculata

Sun

Liu

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

“…T grow better in acidulous medium like some other reference strains such as Flavobacterium acidurans (pH 4.5-6.5) (Millar, 1973), Flavobacterium chungangense (pH 5-8, optimum pH 6) (Kim et al, 2009), Flavobacterium reichenbachii (pH 5.8-8.0, optimum pH 6.2) (Ali et al, 2009) and Flavobacterium aquidurense (pH 6.0-6.6, optimum pH 6.4) (Cousin et al, 2007). Flexirubin-type pigments are positive in the novel strain JJ013 T .…”

Section: The Cells Of Strain Jj013mentioning

confidence: 99%

Flavobacterium aciduliphilum sp. nov., isolated from freshwater, and emended description of the genus Flavobacterium

Kang

Chun

Jahng

2013

International Journal of Systematic and Evolutionary Microbiology

105

View full text Add to dashboard Cite

A Gram-stain-negative, rod-shaped, non-motile and yellow-pigmented bacterial strain, designated strain JJ013 T , was isolated from an artificial lake in Jeollabuk-do, South Korea, and characterized using a polyphasic approach. The 16S rRNA gene sequence of strain JJ013T indicated that the isolate belonged to the family Flavobacteriaceae and exhibited similarity levels of 96.6 % to the type strains of Flavobacterium cheonanense and Flavobacterium koreense and 96.5 % to the type strain of Flavobacterium chungnamense. Growth was observed at 20-30 6C and pH 5.0-7.0. The major cellular fatty acids of the novel strain were iso-C 15 : 0 (27.5 %), iso-C 15 : 1 G (17.8 %), iso-C 17 : 0 3-OH (9.4 %) and iso-C 15 : 0 3-OH (9.2 %). Flexirubin-type pigments were present. The DNA G+C content of strain JJ013 T was 33.9 mol%, the major respiratory quinone was menaquinone-6 (MK-6) and the major polyamine was sym-homospermidine. The polar lipid profile of the strain JJ013 T consisted of a phosphatidylethanolamine (PE), two unknown aminolipids (AL1-2), three unidentified lipid (L1-3) and an unknown glycolipid (GL). On the basis of the morphological and physiological properties and biochemical evidence presented, it is concluded that strain JJ013 T represents a novel species of the genus Flavobacterium, for which the name Flavobacterium aciduliphilum sp. nov. is proposed; the type strain is JJ013 T (5KACC 16594). Since C 15 : 0 , which is known as a predominant fatty acid of the genus Flavobacterium was not detected in the novel strain and other reference strains, we propose an emended description of the genus Flavobacterium.The genus Flavobacterium accommodates Gram-negative, aerobic, rod-shaped and yellow-pigmented bacteria and belongs to the phylum Bacteroidetes. The strains of species of the genus Flavobacterium are found in a variety of environments such as soil, freshwater, seawater, wastewater, Antarctic lakes, gut of earthworm and fish tissues. Most species of the Flavobacterium are sensitive to salts and several species are known to cause diseases in freshwater fish (Bernardet & Bowman, 2006;Wakabayashi et al., 1989). A bacterial strain JJ013T was isolated from the Lake Yongdam in Jinan, Jeollabuk-do, South Korea. The strain appeared as yellow colonies after 4 days cultivation at 25 u C on R2A agar (Difco) and was selected for characterization and sequence analysis of the 16S rRNA gene. For 16S rRNA gene sequencing and G+C content, chromosomal DNA was extracted by using a bacteria genomic DNA isolation kit (RBC). The 16S rRNA gene was amplified from the DNA by PCR (Marchesi et al., 1998) using universal bacterial primers 27F and 1492R. Because the similarity between selected strain and most related strains was lower than 96.6 %, it was studied further. The novel strain was routinely grown on R2A agar at 25 u C for 3 days and preserved at 270 u C as a suspension in R2A broth supplemented with glycerol [20 % (v/v)].The closest phylogenetic neighbours were identified using the EzTaxon database (Kim et al., 2012). Phylogenetic...

show abstract

“…Flavobacterium caeni (Liu et al, 2010), Flavobacterium reichenbachii (Ali et al, 2009), Flavobacterium chungangense (Kim et al, 2009), Flavobacterium cheniae (Qu et al, 2008) and Flavobacterium aquidurense (Cousin et al, 2007). The genus belongs to the family Flavobacteriaceae, phylum 'Bacteroidetes', and, at the time of writing, comprised 89 species, with Flavobacterium aquatile as the type species (Euzéby, 1997).…”

mentioning

confidence: 99%

Flavobacterium ginsenosidimutans sp. nov., a bacterium with ginsenoside converting activity isolated from soil of a ginseng field

Yang

Kim

et al. 2011

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

A Gram-negative, aerobic, non-motile, non-spore-forming, yellow-pigmented, rod-shaped bacterium, designated strain THG 01T, was isolated from the soil of a ginseng field in Pocheon province, South Korea, and its taxonomic position was investigated by using a polyphasic approach. Strain THG 01T grew well at 25–37 °C and pH 6.0–7.5 in the absence of NaCl on nutrient agar. On the basis of 16S rRNA gene sequence similarity data, strain THG 01T was shown to belong to the family Flavobacteriaceae and was related to Flavobacterium anhuiense D3T (97.5 % similarity), Flavobacterium johnsoniae UW101T (96.8 %) and Flavobacterium denitrificans ED5T (96.7 %). 16S rRNA gene sequence similarities between the novel strain and members of other recognized species within the family Flavobacteriaceae were less than 96.7 %. The G+C content of the genomic DNA of strain THG 01T was 32.1 mol%. Phenotypic and chemotaxonomic data (major menaquinone was MK-6 and major fatty acids were iso-C15 : 0, iso-C15 : 0 3-OH and C16 : 1ω7c and/or C16 : 1ω6c ) supported the affiliation of strain THG 01T to the genus Flavobacterium. DNA–DNA hybridization experiments showed that DNA–DNA relatedness values between strain THG 01T and its closest phylogenetic neighbours were below 11 %. The results of physiological and biochemical tests enabled strain THG 01T to be differentiated genotypically and phenotypically from recognized species of the genus Flavobacterium. The isolate therefore represents a novel species, for which the name Flavobacterium ginsenosidimutans sp. nov. is proposed, with THG 01T ( = KACC 14525T = JCM 16720T) as the type strain.

show abstract

Flavobacterium rivuli sp. nov., Flavobacterium subsaxonicum sp. nov., Flavobacterium swingsii sp. nov. and Flavobacterium reichenbachii sp. nov., isolated from a hard water rivulet

Cited by 59 publications

References 18 publications

Flavobacterium suaedae sp. nov., an endophyte isolated from the root of Suaeda corniculata

Flavobacterium suaedae sp. nov., an endophyte isolated from the root of Suaeda corniculata

Flavobacterium aciduliphilum sp. nov., isolated from freshwater, and emended description of the genus Flavobacterium

Flavobacterium ginsenosidimutans sp. nov., a bacterium with ginsenoside converting activity isolated from soil of a ginseng field

Contact Info

Product

Resources

About