Flavonoid characterization and in vitro antioxidant activity of Aconitum anthora L. (Ranunculaceae)

Mariani, Cristina; Braca, Alessandra; Vitalini, Sara; Tommasi, Nunziatina De; Visioli, Francesco; Fico, Gelsomina

doi:10.1016/j.phytochem.2007.12.009

Cited by 73 publications

(60 citation statements)

References 32 publications

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“…OGlycosylation has a slight negative influence at C-7, suggesting a partial role for the free hydroxyl at this position, while at C-3 it has no effect. Furthermore, previous studies reported the radical scavenging properties of aglicons quercetin and their glycosides and of amentoflavone (Ibrahim et al 2007;Mariani et al 2008). Therefore, the determined antioxidant activity of fractions from methanol extract could be related, almost in part, to the presence of the identified flavonoids.…”

Section: Antioxidant Activitymentioning

confidence: 92%

“…Inhibition of a-amylase is important for the resolution of type II diabetes (Mariani et al 2008). In our previous studies (Keskes et al 2014) we have established that the hexane and methanol extracts from J. phoenicea leaves are endowed with a potent a-amylase inhibition activity (IC 50 ¼ 30.15 and 53.76 lg/mL, respectively) ( Table 4).…”

Section: A-amylase Inhibitory Activitymentioning

confidence: 96%

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LC-MS–MS and GC-MS analyses of biologically active extracts and fractions from Tunisian Juniperus phoenice leaves

Keskes

Belhadj

Jlail

et al. 2016

Pharmaceutical Biology

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Context: Despite some studies related to Juniperus phoenicea L. (Cupressaceae), phytochemical and biological investigations of this plant remain unexplored. Objective: This work is the first report dealing with the identification and characterization of volatile components and flavonoids in hexane and methanol extracts from J. phoenicea leaves Materials and methods: Antioxidant activity of hexane, and methanol extracts from J. phoenicea leaves were determined by DPPH-radical scavenging assay. a-Amylase inhibitory activity was evaluated by enzyme inhibition using in vitro assay (each extract was dissolved in DMSO to give concentrations of 50, 100 and 200 mg/mL). The chemical composition of fractions (Fr1-Fr3) from methanol extract was determined by high-performance liquid chromatography coupled with mass spectroscopy (HPLC-MS) analysis. Results and discussion: The hexane extract was analyzed by GC-MS technique which allowed the identification of 32 compounds. The main constituents were a-humulene (16.9%), pentadecane (10.2%) and a-cubebene (9.7%). Fraction Fr 2 exhibited a strong DPPH radical-scavenging activity (IC 50 ¼ 20.1 lg/mL) compared to that of BHT as well as the highest a-amylase inhibitory activity (IC 50 ¼ 28.4 lg/mL). Three flavonoids were identified in these fractions using HPLC-MS analysis: Quercetin 3-O-glucoside, isoscutellarein 7-O-pentoside and quercetin 3-O-pentoside. In addition, the more active fraction (Fr 2) was purified with semi-preparative HPLC affording one pure compound (amentoflavone) using 1 H NMR analysis. This compound exhibited powerful DPPH radical-scavenging (IC 50 ¼ 14.1 lg/mL) and a-amylase inhibition (IC 50 ¼ 20.4 lg/mL) effects. Conclusion: This study provides scientific support to some medicinal uses of J. phoenicea found in North Africa. ARTICLE HISTORY

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Section: Antioxidant Activitymentioning

confidence: 92%

Section: A-amylase Inhibitory Activitymentioning

confidence: 96%

LC-MS–MS and GC-MS analyses of biologically active extracts and fractions from Tunisian Juniperus phoenice leaves

Keskes

Belhadj

Jlail

et al. 2016

Pharmaceutical Biology

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“…Thus, we are interested in the difference of the constituents in B. ternatum and B. virginianum from the viewpoints of the biological activities and chemotaxonomy. Because p-coumarated flavonoid glycosides from Aconitum anthora are reported to have the antioxidant activity, 19) the compounds derived from B. ternatum may also reveal this activity.…”

Section: -[4-hydroxy-(e)-cinnamoyl]-glucopyranosyl-(1 3)]-β-d-6-o-[4-mentioning

confidence: 99%

“…The molecular formulae of ternatumosides VIII (18) and IX (19) were proposed to be C 39 H 50 O 24 and C 39 H 50 O 25 , respectively, based on HR-FAB-MS. The NMR spectroscopic data for 18 and 19 suggested that these compounds were also kaempferol bisdesmosides the same as 13, 14, and 16.…”

mentioning

confidence: 99%

Flavonoid Glycosides from <i>Botrychium ternatum</i>

2012

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The MeOH extract from dried whole Botrychium ternatum plants yielded 33 compounds, including seventeen new flavonoid glycosides and sixteen known compounds. The structures of new compounds were established using NMR spectroscopic analysis and chemical evidence.Key words Botrychium ternatum; Ophiolossaceae; flavonoid glycoside; kaempferol; ternatumoside Recently, plants in the Ophiolossaceaus family were reported to be effective in the cosmetic field, for example, skin moisturizing and lightning actions, improvement and protection of rough skin, and proliferation of skin fibroblasts. 1,2)Botrychium ternatum is one of the Ophiolossaceaus plants and distributed to the Honshu, Shikoku, and Kyushu Islands in Japan, the Korean Peninsula, and China. In Japan, this plant is called "Fuyunohanawarabi" and used to treat dizziness, headache, cough, and fever as a folk medicine. In the course of research to find the efficient agents in the cosmetic field from the Ophiolossaceaus family, we investigated the constituents of B. ternatum and their biological activities.A MeOH extract from dried whole B. ternatum plants was suspended in water. The suspension was extracted with diethyl ether and partitioned into an ether-soluble fraction and a H 2 Osoluble fraction. The ether-soluble fraction was then separated into a n-hexane-soluble fraction and a MeOH-H 2 O (9 : 1)-soluble fraction. The residue of the water soluble-fraction and the MeOH-H 2 O (9 : 1)-soluble fraction was subjected to both silica gel column chromatography and semi-preparative HPLC to give thirty flavonoid glycosides (1-30), two flavonoids (31, 32), and a bibenzyl derivative (33 The molecular formulae of ternatumosides III (8) and IV (9) were identified as C 42 H 46 O 22 and C 51 H 52 O 24 , respectively, by HR-FAB-MS. The NMR spectroscopic data for 8 and 9 were similar to those of 7, and signals due to one and two sets of (trans)-p-coumaroyl groups were observed in 8 and 9, respectively. Additionally, both compounds yielded 7 and (trans)-p-coumaric acid by alkaline hydrolysis.

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“…When quercetin reacts with a free radical, it itself becomes a free radical by donating a proton. This quercetin radical is too low in energy to be reactive due to the delocalization of electrons by resonance [6]. The B ring o-dihydroxyl groups, the 4-oxo group in conjugation with the 2,3-alkene and the 3-and 5-hydroxyl groups is responsible for maintaining quercetin's stability and antioxidant activity when reacting with free radicals [4] (Fig.…”

Section: Structure and Antioxidative Propertymentioning

confidence: 99%