Flavonoids and Some Other Phenolics as Substrates of Peroxidase: Physiological Significance of the Redox Reactions

Takahama, Umeo; Oniki, Takayuki

doi:10.1007/pl00013933

Cited by 93 publications

(60 citation statements)

References 52 publications

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“…Rai et al (2004) stated peroxidase which participate in lignin biosynthesis and might built up a physical barrier against stress response hazards. Soluble, apoplastic PRXs can scavenge H 2 O 2 cooperating with phenolics (Takahama and Oniki 2000). Earlier works pointed out that the first step of antioxidant action is catalyzed by peroxidases where Phytophenolics can be regenerated from phenoxyl radicals by nonenzymatic reaction with ascorbate (reaction 2) inhibiting the formation of degraded products (Yamasaki et al 1997;Sekihama et al 2002;Sgherri et al 2003).…”

Section: Resultsmentioning

confidence: 99%

RETRACTED ARTICLE: Antioxidants and ROS scavenging ability in ten Darjeeling tea clones may serve as markers for selection of potentially adapted clones against abiotic stress

Dasgupta

Biswas

Kumar

et al. 2013

Physiol Mol Biol Plants

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Ten Darjeeling tea clones (BT15/263, RR17/144, B777, T253, B157, Sundaram, HV39, AV2, K1/1 and TTV1) were collected from the experimental garden of Darjeeling Tea Research and Development Centre, Kurseong. Total phenol, flavonoids and two antioxidating enzymes (peroxidase and superoxide dismutase) were estimated. The total phenol ranged between 241 and 28 GAE mg g −1 of leaf dry weight. The highest amount obtained in four clones, B15/263 (241.47), RR17/144 (221.2), B777 (154.54) and B157 (140.23 mg g −1 ). Flavonoids were estimated as Catechin equivalent (CE) and ranged between 56.88 and 20.81 CE mg g −1 leaf dry weight. Higher amounts occurred in BT15/263 (56.88 mg g −1 ), B777 (56.69) and RR17/144 (48.63). Antioxidant activities were measured following DPPH and ABTS free radicle scavenging procedures and the results were well according to total polyphenol content among the clones (in total phenols, ranges of correlation in DPPH assay were r 2 =0.990-0.989, p≤0.05; in flavonoids r 2 =0.954, p≤0.01-0.987, p≤0.05). Similarly, ABTS percent scavenging results were quiet significant. The IC 50 values were determined for both DPPH and ABTS assay. PAGE expressions of isoforms in two antioxidative enzymes and quantification of them also varied much among the investigated clones. The incidence of total phenols, flavonoids, PRX and SOD and ROS scavenging assay in in-situ condition, might be used as biochemical markers towards the superior adaptability against abiotic stress. In the present work, four clones (B15/263, B777, RR17/144 and B157) would be designated as comparatively better suited to the predicted abiotic stress.

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Section: Resultsmentioning

confidence: 99%

RETRACTED ARTICLE: Antioxidants and ROS scavenging ability in ten Darjeeling tea clones may serve as markers for selection of potentially adapted clones against abiotic stress

Dasgupta

Biswas

Kumar

et al. 2013

Physiol Mol Biol Plants

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show abstract

“…A phenolic metabolism in plants can be induced by certain environmental factors, such as stress, injury or infection, and can protect a plant from insectivores (Rees & Harborne 1985;Dixon & Paiva 1995;Takahama & Oniki 2000;Da Cunha et al 2010). Most of these phenolic compounds have antimicrobial, antifeedant and antifungal activity that create a defense mechanism against herbivores and microorganisms (Beckman 2000;Wititsuwannakul et al 2002;Eichhorn et al 2007).…”

Section: Discussionmentioning

confidence: 99%

Leaf structure, microanalysis and characterization of the latex protein profile of Pachystroma longifolium (Nees) I.M. Jonhst. (Euphorbiaceae) in a seasonally dry Atlantic Forest

et al. 2011

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ABSTRACT(Leaf structure, microanalysis and characterization of the latex protein profile of Pachystroma longifolium (Nees) I.M. Jonhst. (Euphorbiaceae) in a seasonally dry Atlantic Forest). Pachystroma longifolium is an evergreen species of Euphorbiaceae that occurs in tabuleiro forest, a type of lowland Atlantic Forest. The site chosen for this study was a fragment of tabuleiro forest that has a history of being selectively logged for timber. P. longifolium is very successful at colonizing disturbed areas in this forest fragment in comparison with preserved fragments. In the present work, which was based on using different microscopy (electron and light microscopy, X-ray analysis) and biochemistry techniques, we describe the leaf and latex traits of P. longifolium and their role as defense mechanisms against desiccation and herbivory. Our results suggest the richness of P. longifolium populations, in the disturbed forest area studied, is primarily a consequence of unpalatable leaves because of an abundance of calcium oxalate crystals in the subjacent epidermis; the presence of phenolic compounds in the adaxial surface; and a laticifer system that contains a complex mixture of proteins, which provides resistance to herbivores. In addition, this species is resistant to desiccation during dry periods because of an extensive amount of wax that occurs on the outer cell walls of the epidermis, and its ability to retain water because of a biseriate epidermis.

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“…1997. The an ti ox i dant abil ity was also sup posed to ex ist in plants (Takahama et al 2000). Due to our lack in un der stand ing of flavonoid func tion in plants, further stud ies would be worth while.…”

Section: Introductionmentioning

confidence: 94%

Effects of quercetin and enhanced UV-B radiation on the soybean (Glycine max) leaves

Qun

2006

Acta Physiol Plant

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Key words: Soy bean (Glycine max); UV-B ra di ation; quercetin; ox i da tive stress Ab stractThe pos si ble ameliorative ef fects of quercetin on soy bean [Glycine max (L.) Merr.] leaves ex posed to UV-B ra di a tion were con ducted in green house. The sym met ri cal leaves supplied with quercetin so lu tion (0.2 %, 1 %) were ex posed to UV-B ra di a tion (0, 3.5, 6.5 kJ·m -2 ·d -1 ). 0.2 % quercetin amelio rated leaf pho to syn the sis, im proved leaf wa ter con tent (LWC), and de creased lipid ox i da tion. The un fa vor able ef fect on photosynthetic pa ram e ter was dis played in 1 % quercetin treat ment. The ef fect of quercetin on phenylalanine am mo nia lyase (PAL) ac tiv ity var ied with the quercetin con cen tra tion, UV-B ra di a tion in ten sity and leaf de vel op ment. In the later devel op ment polyphenol oxidase (PPO) ac tiv ity was in creased sig nif i cantly by quercetin treat ments. We sug gested that quercetin with suit able con cen tra tion could serve as UV-B pro tec tive agent partly due to its an ti ox i dant ca pac ity. List of ab bre vi a tions: UV-B ul tra vi o let-B; NPRNet photosynthetic rate; MDA malondialdehyde; PAL phenylalanine ammonialyase; SOD superoxide dismutase; PPO polyphenol oxidase; LWC leaf wa ter con tent; ROS re ac tive ox y gen spe cies In tro duc tionThe in creased lev els of ul tra vi o let-B (UV-B) ra di ation reach ing the Earth sur face due to the de ple tion of the strato spheric ozone layer (Madronich et al. 1998) prompted ac tive re search on how plant organs were pro tected by dif fer ent mech a nisms (Roxema et al. 1997, Gotz et al. 1999. The plant an ti ox i dant en zyme ac tiv i ties was in creased to elim i nate re ac tive ox y gen spe cies (ROS), the leaf ca rot en oids con tents were in creased to pro tect photosynthetic chain, and leaf flavonoid con tent was also in duced to screen the UV-B ra di a tion. In re cent years, a se ries of ex per i ments pro vided convinc ing ev i dence that plants sub jected to UV-B radi a tion re sponded to changes in the con tent and ratios of dif fer ent flavonoid in leaf epi der mal cells, wax, hairs (Harborne et al. 2000). Some flavonoid in creased much more than oth ers (Noriaki et al. 2000), es pe cially the flavonoid with ortho-hydroxy struc ture in B-ring such as quercetin and quercetin glycoside in Bras sica napus and Trifolium repens

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Flavonoids and Some Other Phenolics as Substrates of Peroxidase: Physiological Significance of the Redox Reactions

Cited by 93 publications

References 52 publications

RETRACTED ARTICLE: Antioxidants and ROS scavenging ability in ten Darjeeling tea clones may serve as markers for selection of potentially adapted clones against abiotic stress

RETRACTED ARTICLE: Antioxidants and ROS scavenging ability in ten Darjeeling tea clones may serve as markers for selection of potentially adapted clones against abiotic stress

Leaf structure, microanalysis and characterization of the latex protein profile of Pachystroma longifolium (Nees) I.M. Jonhst. (Euphorbiaceae) in a seasonally dry Atlantic Forest

Effects of quercetin and enhanced UV-B radiation on the soybean (Glycine max) leaves

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