Flavonoids of Rosa roxburghii Tratt offers protection against radiation induced apoptosis and inflammation in mouse thymus

Abstract

The present study evaluated the protective effect of the natural compound flavonoids of Rosa roxburghii Tratt (FRT) against γ-radiation-induced apoptosis and inflammation in mouse thymus cells in vivo and in vitro. Thymus cells and mice were exposed to Co γ-ray at a dose of 6 Gy. The radiation treatment induced significant cell apoptosis and inflammation. Radiation increased the expressions of cleaved caspase 3/8-10, AIF, and PARP-1, and FRT could mitigate their activation and inhibit subsequent apoptosis in the thymus both in vitro or in vivo. Irradiation increased the mRNA expression of ICAM-1/VCAM-1, IL-1α/IL-6 and TNF-α/NF-κB. Our results also indicated that FRT alleviated gene expression of some inflammatory factors such as ICAM-1/VCAM-1, TNF-α/NF-κB, but not IL-1α/IL-6. Irradiation increased the protein expression levels of ICAM-1/VCAM-1, IL-1α/IL-6 and TNF-α/NF-Κb, and our results also indicated that FRT alleviated protein level expression of certain inflammatory factors such as ICAM-1, IL-1α/IL-6, TNF-α/NF-κB, but not VCAM-1. Our results suggested that FRT enhanced radioprotection at least partially by regulating caspase 3/8-10, AIF, and PARP-1 to reduce apoptosis and by regulating ICAM-1, IL-1α/IL-6, TNF-α/NF-κB to reduce inflammation.

Optimization of Ultrasound Assisted Extraction (UAE) of Kinsenoside Compound from Anoectochilus roxburghii (Wall.) Lindl by Response Surface Methodology (RSM)

“…Ito et al [4] first reported the isolation and structural characterization of it, which exists in Anoectochilus koshunensis and Anoectochilus formosanus. Researchers have found that kinsenoside had a different multiple pharmacologic activity compared with polysaccharide and flavonoids [5][6][7][8]. As a potential immunosuppressive drug for autoimmune hepatitis, it has a vascular protective effect under high glucose conditions and has been found to enhance the oxidative resistance of diabetic mice [9][10][11].…”

Flavonoids from Rosa roxburghii Tratt prevent reactive oxygen species-mediated DNA damage in thymus cells both combined with and without PARP-1 expression after exposure to radiation in vivo

“…Flavonoids have been demonstrated to exhibit significant radioprotective capabilities [ 27 ]. Previous studies have shown that FRT reduces apoptosis by regulating caspase 3/8-10, AIF and PARP-1, with inflammatory reactions suppressed through regulation of ICAM-1, IL-1α/IL-6β and TNF-α/NF-КB, thereby enhancing radiation protection [ 28 ]. The aim of this study was to explore the effect of FRT on KO mice including the relationship between PARP-1 and ROS, also ROS and DNA following their exposure to radiation.…”

The Anti-Aging Activities against Oxidative Damages of <i>Rosa roxburghii</i> and Multi-Fruit Concentrate Drink

“…It is rich in polyphenols, flavonoids, organic acids, triterpenes, amino acids, polysaccharides and essential oils [23,24,25]. All these elements allow R. roxburghii to deliver a range of health benefits including anti-oxidation, anti-mutagenesis, anti-inflammation, anti-aging and anti-tumor effects [26,27,28,29]. Notably, R. roxburghii has been proved to enhance the expression of anti-oxidative, DNA mismatch repair, and telomere maintenance (i.e., XRCC5) genes in macrophage cells, meaning that R. roxburghii can improve the anti-oxidative capability, reduce the possibility of cancer formation, and prolong the life span in cells [30].…”