Flavonoids ofArmoracia rusticana andBarbarea arcuata

Fursa, N. S.; Литвиненко, В. И.; Krivenchuk, P. E.

doi:10.1007/bf00683854

Cited by 5 publications

(2 citation statements)

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“…On the other hand, little is known about the poliphenolic composition of the horseradish. Based on earlier research Armoracia rustucana contains a small amount of flavonoids, mainly kaempferol and quercetin 20 . Cirimbei and associates demonstrated great potential of the aqueous plant extract from A. rusticana and its main flavonoids, kaemferol and quercetin, to protect DNA from damage induced on human lymphocytes by the oxidative agent hydrogen peroxide 21 .…”

Section: Introductionmentioning

confidence: 99%

Polyphenol rich horseradish root extracts and juice: In vitro antitumor activity and mechanism of action

et al. 2021

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Background/Aim. Plant poliphenols are well known to show antimutagenic, anticarcinogenic, antiviral and antioxidative activity. The aim of this study was to investigate bioactive potential of Armoracia rusticana root juice and extractstheir polyphenol content, as well as in vitro antitumor activity and cell-death mechanism. Methods. Liquid-liquid extraction of polar and non-polar compounds was used and polyphenolic compounds were identified and quantified by HPLC analysis. Antiproliferative activity was examinated in vitro on human cervix carcinoma (HeLa), breast adenocarcinoma (MCF7, MDA-MB-231), colon adenocarcinoma (HT-29), lung adenocarcinoma (A549), prostate adenocarcinoma (PC-3), melanocyte carcinoma (Hs 294T), hepatocyte carcinoma (Hep G2), as well as rat hepatocyte carcinoma (H-4-II-E), and normal human fetal lung (MRC-5) cell line using sulforhodamine B assay. The mechanism of cell-death in cell line was determinated using Cell Death Detection ELISA PLUS kit. Results. Dichloromethane extracts had the highest content of catechin, phydroxybenzoic, syringic and gallic acid (pulp, E1), and epicatechin (juice, E3). The results showed strong and non-selective antiproliferative activity of chloroform and dichloromethane extracts and root juice-highest being towards liver, breast and lung tissue cells. IC50 values of extracts and juice were obtained in low range of concentrations (IC50=3.49-26.5 µg/mL) and high range of dilutions (IC50=418-1590). High and unfavorable potential of horseradish juice and chloroform juice extract (E4) to induce necrotic cell death was detected. Conclusion. Strong and non-selective in vitro antiproliferative activity of chloroform and dichloromethane extracts and root juice of horseradish was detected, with necrosis as a main mechanism of induced cell death. In order to utilize horseradish root bioactive potential further investigations that will pinpoint active components with more favourable apoptosis/necrosis inducing properties are needed.

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Section: Introductionmentioning

confidence: 99%

Polyphenol rich horseradish root extracts and juice: In vitro antitumor activity and mechanism of action

et al. 2021

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“…Ascorbic acid is a very strong antioxidant and it also plays a role in collagen synthesis [11,12,13]. Armoracia rusticana contains a small amount of flavonoids – kaempferol and quercetin [14,15,16,17,18].…”

Section: Introductionmentioning

confidence: 99%

Extract from Armoracia rusticana and Its Flavonoid Components Protect Human Lymphocytes against Oxidative Damage Induced by Hydrogen Peroxide

et al. 2014

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DNA damage prevention is an important mechanism involved in cancer prevention by dietary compounds. Armoracia rusticana is cultivated mainly for its roots that are used in the human diet as a pungent spice. The roots represent rich sources of biologically active phytocompounds, which are beneficial for humans. In this study we investigated the modulation of H 2 O 2 genotoxicity using the A. rusticana root aqueous extract (AE) and two flavonoids (kaempferol or quercetin). Human lymphocytes pre-treated with AE, kaempferol and quercetin were challenged with H 2 O 2 and the DNA damage was assessed by the comet assay. At first we assessed a non-genotoxic concentration of AE and flavonoids, respectively. In lymphocytes challenged with H 2 O 2 we proved that the 0.0025 mg·mL ) significantly diminished oxidative DNA damage (from 83.3% to 19.4%), and the same concentration of quercetin also reduced the genotoxic effect of H 2 O 2 (from 83.3% to 16.2%). We conclude that AE, kaempferol and quercetin probably act as antimutagens. The molecular mechanisms underlying their antimutagenic activity might be explained by their antioxidant properties.

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Flavonole und Flavone der Gem�searten V. Flavonole und Flavone des Wurzelgem�ses

Eloesser

Herrmann

1975

Z Lebensm Unters Forch

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Root vegetables contain flavon(ol) glycosides in tracers up to small amounts, while the level of their leaves are in part considerable (to more than 1 g/kg, calculated as aglycon). Radish, rutabagas, scorzoneras, and beets contain less than 1 mg/kg kaempferol and/or quercetin; carrots less than 1 mg/kg apigenin and luteolin; celery roots ca. 75 mg apigenin/kg and 14 mg luteolin/kg; horseradish about 20 mg kaempferol/kg and small radish 1-10 mg kaempferol/kg, whereby all these flavones and flavonols occur as glycosides in the vegetables. In leaves of small radish, variety "Eiszapfen", we found besides isoquercitrin (quercetin-3-glucoside) a quercetin-3-0-diglycoside and a kaempferol-0-diglycoside, both with the sugars rhamnose and arabinose, by tlc.

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Flavonoids ofArmoracia rusticana andBarbarea arcuata

Cited by 5 publications

References 1 publication

Polyphenol rich horseradish root extracts and juice: In vitro antitumor activity and mechanism of action

Polyphenol rich horseradish root extracts and juice: In vitro antitumor activity and mechanism of action

Extract from Armoracia rusticana and Its Flavonoid Components Protect Human Lymphocytes against Oxidative Damage Induced by Hydrogen Peroxide

Flavonole und Flavone der Gem�searten V. Flavonole und Flavone des Wurzelgem�ses

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