Flow cytometry and integrated imaging

Kachel, Volker; Wietzorrek, Joachim

doi:10.3989/scimar.2000.64n2247

Cited by 20 publications

(12 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This does not seem realistic for the near future, at least until we have more insight into the possibilities of low power fast acquisition, storage and subsequently highly efficient data reduction of images. Imaging generates massive amounts of data (Kachel and Wietzorrek, 2000). A moderate resolution of 256x256 pixels uses 64 kbyte for a single particle, which is the present full grabber capacity.…”

Section: Pursuit Of a Higher Analytical Resolutionmentioning

confidence: 99%

CytoBuoy: a step forward towards using flow cytometry in operational oceanography

Dubelaar¹,

Gerritzen²

2000

Sci. Mar.

116

View full text Add to dashboard Cite

Section: Pursuit Of a Higher Analytical Resolutionmentioning

confidence: 99%

CytoBuoy: a step forward towards using flow cytometry in operational oceanography

Dubelaar¹,

Gerritzen²

2000

Sci. Mar.

116

View full text Add to dashboard Cite

“…Previous work in the last decade demonstrated feasibility of integrating video imaging with flow cytometry in laboratory-based systems for study of plankton and other microbes (Sieracki et al, 1998;Kachel and Wietzorrek, 2000;Brehm-Stecher, 2007). When combined with the growing expertise in extended underwater flow cytometry and with new automated image analysis and classification approaches (e.g., Sosik and Olson, 2007), this capability can provide unique observations of plankton communities at ecologically relevant scales.…”

Section: Flow Cytometrymentioning

confidence: 99%

Optical tools for ocean monitoring and research

et al. 2009

View full text Add to dashboard Cite

Abstract. Requirements for understanding the relationships between ocean color and suspended and dissolved materials within the water column, and a rapidly emerging photonics and materials technology base for performing optical based analytical techniques have generated a diverse offering of commercial sensors and research prototypes that perform optical measurements in water. Through inversion, these tools are now being used to determine a diverse set of related biogeochemical and physical parameters. Techniques engaged include measurement of the solar radiance distribution, absorption, scattering, stimulated fluorescence, flow cytometry, and various spectroscopy methods. Selective membranes and other techniques for material isolation further enhance specificity, leading to sensors for measurement of dissolved oxygen, methane, carbon dioxide, common nutrients and a variety of other parameters. Scientists are using these measurements to infer information related to an increasing set of parameters and wide range of applications over relevant scales in space and time.

show abstract

“…The optical plankton analyser (OPA) was developed for field samples (Balfoort et al, 1992a;Dubelaar et al, 1989) containing single cells and colonies, including aggregates and filaments with lengths over a millimetre, being measured by the OPA with fair linearity (Dubelaar and van der Reijden, 1995). In the subsequent EurOPA project (Dubelaar et al, 1995b), a more versatile instrument was developed with a photodetector array probing diffracted light, a pulse profile acquisition module (Cunningham, 1990b), a cytometric imaging device (Wietzorrek, 1994;Kachel and Wietzorrek, 2000) and a sorter system. Cavender- Bares et al (1998) developed a dual sheath flow cytometer for shipboard analyses to cope with widely varying and very low concentrations of phytoplankton in the oligotrophic oceans.…”

Section: Limitations and Pitfalls With Phytoplankton Samples Dedicatmentioning

confidence: 99%

Flow cytometry as a tool for the study of phytoplankton

Dubelaar¹,

Jonker²

2000

Sci. Mar.

View full text Add to dashboard Cite

SUMMARY: An overview is presented on flow cytometry as a tool for counting, analysis and identification of phytoplankton species and groups. The paper covers basics on the analysis technique and instrumentation such as the measuring principle, the type of instrument, limitations and pitfalls with phytoplankton samples and sample handling and preprocessing. Possibilities of the measured entities are discussed, roughly divided in light scatter and related parameters, the endogenous fluorescence and exogenous fluorescence, followed by a discussion on the actual applications such as phytoplankton abundance analysis, ecology and physiology research and monitoring of particle size and biomass. In addition to a limited literature review, we tried to assess how flow cytometry is used in routine laboratory practice and monitoring operations. Therefore, a questionnaire was sent out via email to 47 scientists at 43 institutes known to us as involved in flow cytometric analysis of phytoplankton. In total, 19 scientists responded. Specific survey results are included in italic print whereas some more general answers were integrated in the overview.

show abstract

Flow cytometry and integrated imaging

Cited by 20 publications

References 5 publications

CytoBuoy: a step forward towards using flow cytometry in operational oceanography

CytoBuoy: a step forward towards using flow cytometry in operational oceanography

Optical tools for ocean monitoring and research

Flow cytometry as a tool for the study of phytoplankton

Contact Info

Product

Resources

About