Flow injection–fluorimetric method for the determination of ranitidine in pharmaceutical preparations using o-phthalaldehyde

Abstract

A flow injection-fluorimetric procedure for the determination of ranitidine is proposed. The assay is based on the reaction of the drug with sodium hypochlorite, to produce a primary amine, which reacts with o-phthalaldehyde and 2-mercaptoethanol to form highly fluorescent derivatives. The calibration graph, based on peak area, is linear in the range 20-500 ng ml-l of ranitidine with an accuracy of 3.4%. The corresponding detection limit is 13 ng ml-l (3.7 pmol). The method was applied to the determination of ranitidine in pharmaceuticals. The recovery was quantitative and no interferences from excipients were observed.

Amperometric Detection of Ranitidine Using Glassy Carbon Modified with Ruthenium Oxide Hexacyanoferrate Adapted in a Flow Injection System

“…In the literature, there are different methods for quantification of ranitidine using the following techniques: spectrophotometry [3][4][5][6][7][8][9][10][11], fluorimetry [7,12,13], chemiluminescence [14,15], spectrometry [16], electrochemistry [11,[17][18][19][20][21][22][23][24][25] and chromatography [26][27][28]. In most cases, using the methods of spectrophotometry, spectrometry, fluorimetry and chemiluminescence a preliminary step of derivatization is necessary, which will result in longer analysis times, an example of which is the precipitation of ranitidine with Co and Cd complex prior to analysis by spectrometry [16].…”

Recent progress in pharmacokinetic applications of capillary electrophoresis

“…Both drugs are hydrophilic molecules containing a substituted furano ring (RANT) or a substituted thiazol ring (FAMT). Methods used for determining RANT and FAMT include UV/VIS spectrometry [56][57][58], polarography [59][60][61], flow-injection analysis with spectrophotometric, potentiometric, fluorimetric, and chemiluminimetric detection [62][63][64]. These methods, however, are not adaptable for use in pharmacokinetic studies because of their lack of selectivity.…”

New reagents for the spectrophotometric determination of ranitidine hydrochloride

“…Thus measuring the amount of ranitidine N-oxide present in urine after administration of ranitidine has been used as a non-invasive method of determining the human liver FMO activity in vivo [8]. Several techniques such as proton magnetic resonance spectroscopy [9], near infrared refl ectance spectrometry [10], scintillation proximity assay [11], fl ow injection fl uorimetry [12], polarography [13,14], differential pulse polarography [15], capillary electrophoresis [16], high performance liquid chromatography [17,18] have been used for the determination of RNH in pharmaceuticals. These techniques require sophisticated instruments and expensive reagents, and involve several manipulation steps and derivatization reactions.…”