Flow injection spectrophotometric determination of the antibiotic ciprofloxacin in drug formulations

Abstract

A flow injection spectrophotometric method for the assay of ciprofloxacin was developed. The method was based on the chelation of iron(III) with the drug in 0.023 mol dm-3 sulfuric acid solution in a 72 cm long coil and the brown-red complex produced was monitored at 447 nm. The super modified simplex computer program was employed for the optimization of the system and chemical parameters with respect to throughput and sensitivity as a measure of system performance. A working range for ciprofloxacin determination of 50-500 ppm for a 110 mm3 sample size with an optimum of 250 samples per hour was achieved with a relative standard deviation of less than 0.92%. The method was successfully applied to the determination of ciprofloxacin in drug formulations.

Cloud Point Extraction Spectrophotometric Method for Mutual Determination of Norfloxacin and Iron(III) in Human Serum and Drug Formulations

“…The Job plot (continuous variation method) was also confirmed similar result to molar ratio method which exhibited that the mole fraction close to 0.50, indicating again that the ratio of iron(III):drug in the complex is 1:2 as displayed in Figure 9. 27 , Suliman and Sultan 25 , but they do not agree with the results achieved by other workers 28 where a 1: 1 complex was obtained, when iron(III) was complexed with ciprofloxacin or norfloxacin at acidities higher than 0.025 M. By assuming only a single complex is present, the stability constant (K f ) of the Fe(III)-NOR complex can be determined according to the procedure adopted elsewhere 29 by using the above data and found to be of 2.12×10 10 at 432 nm. On the basis of above results, the most probable structure of the complex formed between Fe(III) and NOR in dilute acidic medium is displayed in Figure 10.…”

A First-Derivative Spectrophotometric Method for the Determination of Ciprofloxacin Hydrochloride in Ophthalmic Solution

“…The reaction between iron (III) and ciprofloxacin is a fast reaction, in which Fe 3+ react with the active carboxylic acid and the adjacent keto group in the 3-and 4-position, respectively, thus forming a six-membered ring [16]. In this reaction, the yellow coloration reaches maximum intensity immediately at room temperature and remains stables for at least 60 min.…”

“…For this reason, spectrophotometric techniques continues to be useful alternatives for the assay of different classes of drugs in pharmaceutical formulations and in biological samples due to its simplicity, speed, precision, accuracy, Although there are studies reporting the use of Fe(III) as colorimetric reagent for the spectrophotometric determinati on of ciprofloxacin [16,[21][22], there are no reports on the use of Fe(III) complexes for determination of the studied compound by derivative spectrophotometry. We studied the reaction of hydrochloride ciprofloxacin with Fe(III) in an attempt to develop a simple and sensitive method as an interesting alternative to those existing in the literature.…”

Preconcentration and Determination of Ciprofloxacin with Solid-phase Microextraction and Silica-coated Magnetic Nanoparticles Modified with Salicylic Acid by UV-Vis Spectrophotometry

“…Other different methods were reported such as spectrophotometry [16][17][18][19][20][21][22], fluorimetry [22][23][24], capillary electrophoresis [25][26][27][28][29][30][31][32] and immunoassay [33,34]. Both spectrophotometric [35][36][37][38] and chemiluminescent [39,40] detection were proposed as automatic spectroscopy procedures.…”