Flow-Injection System with Site-Specific Immobilization of Acetylcholinesterase Biosensor for Amperometric Detection of Organophosphate Pesticides

Overview on Biosensors for Detection of Organophosphate Pesticides

“…The detection of malathion was proportional to its inhibition concentration ranging from 0.1nM to 1.0nM and 1.0nM to 10nM, with a detection limit of 0.04nM. Ivanov et al [44] reported a flow-injection system with integrated amperometric biosensor featuring an easily replaceable immobilized AChE membrane. The amperometric biosensor was constructed on the basis of site-specific immobilization of AChE on a hybrid polymer membrane with integrated MWCNTs.…”

Protein Surface Structural Recognition in Inactive Areas: A New Immobilization Strategy for Acetylcholinesterase

“…Compared to the conventional methods of enzyme immobilization, site-specific enzyme immobilization effectively controls the orientation of immobilized enzymes and avoids reducing protein function by steric hindrance of available bioactive sites and reduction of protein stability . Current methods for site-specific enzyme immobilization create (bio)­affinity bonds between an activated support and a specific group of the protein sequence. , Typically these methods rely on the affinity between the enzyme and specific molecules or groups (e.g., sugars, biotin, metal chelates, histidine or cysteine, azide moiety, peptides). − For AChE, the research of site-specific enzyme immobilization has only recently begun, and only a few papers on the methods of site-specific immobilization of AChE based on the overall structural characteristics (surface charge or hydrophobicity) have been reported. − Ivanov et al described how site-specific immobilization of AChE on chemically modified poly­(acrylonitrile-methyl-methacrylate-sodium vinylsulfonate) membranes could be used for the development of an amperometric biosensor. Other papers have shown that the site-specific immobilization enzymes were used to detect a variety of pesticides. , Ganesana et al reported that the site-specific affinity immobilization of (His)­6-tagged AChE on Ni/NiO nanoparticles in the development of an electrochemical screen-printed biosensor could be used for the detection of paraoxon .…”

“…Other papers have shown that the site-specific immobilization enzymes were used to detect a variety of pesticides. 23,24 Ganesana et al reported that the sitespecific affinity immobilization of (His)6-tagged AChE on Ni/ NiO nanoparticles in the development of an electrochemical screen-printed biosensor could be used for the detection of paraoxon. 22 Chumphukam et al found a method of site-specific affinity immobilization AChE using a DNA aptamer that binds AChE with high affinity.…”

A highly sensitive electrochemiluminescence biosensor for the detection of organophosphate pesticides based on cyclodextrin functionalized graphitic carbon nitride and enzyme inhibition

“…= 1968.7 lg(c/pM) + 1368.8 (where I was the ECL intensity and c was the concentration of OPs), with a correlation coefficient R 2 = 0.996. Compared with other biosensors for OPs detection (Table S1, ESI † [28][29][30][31] ), the proposed biosensor exhibited a lower detection limit of 0.3 pM (S/N = 3), and the linear range was from 1.0 pM to 0.5 mM, achieving six orders of magnitude, which might hold a new promise for the highly sensitive and ultratrace detection of OPs. In addition, the excellent stability and reproducibility of the biosensor are exhibited in Fig.…”