Overview on Biosensors for Detection of Organophosphate Pesticides

Melo, Jose Savio

doi:10.19080/ctbeb.2017.05.555663

Cited by 10 publications

(4 citation statements)

References 55 publications

(73 reference statements)

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“…1 Among the class of pesticides, organophosphate-based pesticides are more deleterious due to the irreversible inhibition of the acetylcholinesterase enzyme, which regulates acetylthiocholine, a neuro-transmitting agent. 2,3 The adverse effects include respiratory disorder, muscular dystrophy, neurological ailments, and sometimes death. 4−6 The above-stated consequences of the use of organophosphates (OPs) have motivated the scientific community to develop various analytical techniques for its quantitative or qualitative detection.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Food and environmental contamination due to pesticides is increasing enormously for the past few decades . Among the class of pesticides, organophosphate-based pesticides are more deleterious due to the irreversible inhibition of the acetylcholinesterase enzyme, which regulates acetylthiocholine, a neuro-transmitting agent. , The adverse effects include respiratory disorder, muscular dystrophy, neurological ailments, and sometimes death. − The above-stated consequences of the use of organophosphates (OPs) have motivated the scientific community to develop various analytical techniques for its quantitative or qualitative detection. The conventional analytical techniques available for the detection of OPs are high-performance liquid chromatography (HPLC), liquid chromatography (LC), mass spectroscopy (MS), gas chromatography-mass spectroscopy (GC-MS), enzyme-linked immunosorbent assay (ELISA), and other enzymatic immunoassays. , The aforementioned techniques require a sophisticated analytical laboratory, cumbersome chemical processes, large sample volumes, and skilled manpower and also are costly. − …”

Section: Introductionmentioning

confidence: 99%

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Microfluidic Affinity Sensor Based on a Molecularly Imprinted Polymer for Ultrasensitive Detection of Chlorpyrifos

et al. 2020

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The persistent use of pesticides in the agriculture field remains a serious issue related to public health. In the present work, molecularly imprinted polymer thin films were developed using electropolymerization of pyrrole (py) onto gold microelectrodes followed by electrodeposition for the selective detection of chlorpyrifos (CPF). The molecularly imprinted polymer (MIP) was synthesized by the electrochemical deposition method, which allowed in-line transfer of MIP on gold microelectrodes without using any additional adhering agents. Various parameters such as pH, monomer ratio, scan rate, and deposition cycle were optimized for sensor fabrication. The sensor was characterized at every stage of fabrication using various spectroscopic, microscopic, and electrochemical techniques. The sensor requires only 2 μL of the analyte and its linear detection range was found to be 1 μM to 1 fM. The developed sensor’s limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.93 and 2.82 fM, respectively, with a sensitivity of 3.98 (μA/(μM)/ mm2. The sensor’s shelf life was tested for 70 days. The applicability of the sensor in detecting CPF in fruit and vegetable samples was also assessed out with recovery % between 91 and 97% (RSD < 5%). The developed sensor possesses a huge commercial potential for on-field monitoring of pesticides.

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Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Microfluidic Affinity Sensor Based on a Molecularly Imprinted Polymer for Ultrasensitive Detection of Chlorpyrifos

et al. 2020

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“…However, the use of pesticides has the potential to harm the environment and human health due to their nature which is difficult to decompose, so they become toxic substances (Kaur et al, 2013;Sahub et al, 2018). Therefore, the Environmental Protection Agency (EPA) took steps to solve this problem by detecting pesticides (Kumar & Melo, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…Biosensors are an alternative method that has become popular and is of research interest. As technology and science develop, researchers have tried to develop and innovate to improve the performance of biosensors, especially the sensitivity and stability values (Kumar & Melo, 2017).…”

Section: Introductionmentioning

confidence: 99%

A Novel Enzyme Biosensor Based on Ag/Reduced Graphene Oxide/Chitosan Membrane with Potentiometer for Pesticide Detection

Mashuni

Yanti

Irnawati

et al. 2023

molekul

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Long-term accumulation of pesticides in the environment to human and animal health. Acetylcholinesterase (AChE) biosensors with highly sensitive potentiometer transducers based on the membranes of Ag, reduced graphene oxide (rGO), and chitosan (CS) has been successfully developed. The membrane was made with a composition of 0.5 mM AgNO3, 2.5 mg/mL rGO, and 2% (w/v) CS coated on the surface of the Au electrode. The composition of the membrane with three ratios, namely 1:1:2, 2:1:3, and 3:1:4. Then, membrane Ag/rGO/CS and the enzyme AChE were immobilized on the membrane surface. The prepared biosensor has excellent conductivity, catalytic activity, and biocompatibility attributed to the synergistic effect of Ag/rGO/CS and glutaraldehyde (GTA) as crosslinkers and providing a hydrophilic surface for AChE adhesion. The linear range in biosensors is 1 × 10-8 to 1 µg L-1 with a regression coefficient of 0.9803 for 1:1:2 membrane, 0.9836 for 2:1:3 membrane, and 0.9850 for 3:1:4 membrane. The LOD is about 1 × 10-7 µg L-1 for all membranes. In addition, the biosensor showed good sensitivity, acceptable reproducibility, and stability, having an RSD of less than 5%. This biosensor makes it possible to provide a new and promising tool for analyzing pesticides, especially organophosphates.

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