Flow Mediated Skin Fluorescence technique reveals remarkable effect of age on microcirculation and metabolic regulation in type 1 diabetes

Katarzyńska, Joanna; Borkowska, Anna; Czajkowski, Przemyslaw; Łoś, Agnieszka; Szczerbiński, Łukasz; Milewska-Kranc, Agnieszka; Marcinek, Andrzej; Krętowski, Adam; Cypryk, Katarzyna; Gębicki, Jerzy

doi:10.1016/j.mvr.2019.02.005

Cited by 29 publications

(29 citation statements)

References 20 publications

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“…FMSF measures changes in NADH fluorescence intensity as a response to blocking and releasing blood flow in the forearm, as a function of time. The FMSF technique has been successfully applied for non-invasive diagnostics and monitoring of the microcirculation, metabolic regulation, and vascular complications in diabetes patients (Katarzynska et al, 2019b(Katarzynska et al, , 2020a. Very recently, the FMSF technique was used to monitor microcirculatory oscillations known as flowmotion (Katarzynska et al, 2020b).…”

Section: Contents Lists Available At Sciencedirectmentioning

confidence: 99%

Can the microcirculatory response to hypoxia be a prognostic factor for Covid-19?

Gębicki

Katarzyńska²,

Marcinek

2020

Respiratory Physiology & Neurobiology

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confidence: 99%

Can the microcirculatory response to hypoxia be a prognostic factor for Covid-19?

Gębicki

Katarzyńska²,

Marcinek

2020

Respiratory Physiology & Neurobiology

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“…and reaches maximum (FL max) in response to ischemia and the accumulation of NADH in keratinocytes. Results are displayed as ischemic response maximum (IR max) and ischemic response area under the curve (IR auc) [17]. Finally, a reperfusion (hyperaemic) stage takes place following sphygmomanometer release: NADH fluorescence rapidly decreases and reaches minimum (FL min) within the first few seconds of reperfusion and then recovers, less steeply, to the baseline level within 4.5 minutes.…”

Section: Methodsmentioning

confidence: 99%

“…Finally, a reperfusion (hyperaemic) stage takes place following sphygmomanometer release: NADH fluorescence rapidly decreases and reaches minimum (FL min) within the first few seconds of reperfusion and then recovers, less steeply, to the baseline level within 4.5 minutes. Results are displayed as hyperemic response max (HR max) and hyperemic response area under the curve (HR auc) [17]. To illustrate, typical results and definitions of measured parameters, obtained in one individual of the healthy control group, are presented in Figure 1.…”

Section: Methodsmentioning

confidence: 99%

Changes of NADH Fluorescence from the Skin of Patients with Systemic Lupus Erythematosus

Bogaczewicz

Tokarska

Woźniacka

2019

BioMed Research International

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Introduction. The blood circulation of the skin is an accessible and representative vascular bed for examining the mechanisms of microcirculatory function. Endothelial function is impaired in systemic lupus erythematosus (SLE), which implies disorders in cell metabolism dependent on blood circulation; however, noninvasive monitoring of metabolism at the tissue and cell level is absent in daily clinical practice. Objective. The aim of the study was to examine changes of NADH fluorescence from the epidermis of a forearm measured with the flow mediated skin fluorescence (FMSF) technique in patients with SLE and to investigate whether they are associated with clinical manifestation of the disease. Materials and Methods. The study enrolled 36 patients with SLE and 34 healthy individuals. Changes of NADH fluorescence were measured using FMSF on the forearm in response to blocking and releasing of blood flow. The results were represented as ischemic (IR max and IR auc) and hyperemic response maximum and area under the curve (HR max and HR auc). Results. IR max, IR auc, HR max, and HR auc were all lower in patients with SLE (p<0.05) compared with controls. All four parameters were negatively correlated (p<0.05) with patient age. No difference was found in NADH fluorescence between SLE patients with malar rash, discoid rash, photosensitivity, oral ulcers, nonerosive arthritis, renal disorder, hematologic disorder, or immunologic disorder and those without. No correlation was revealed between the SLEDAI score and NADH fluorescence. Conclusion. Changes of NADH fluorescence indicate the reduction in NADH restoration, observed especially during reperfusion, and suggest the occurrence of disorders in the microcirculation of the skin and/or at the mitochondrial level. Such changes of NADH during reperfusion in patients with SLE could be associated with their possible lower sensitivity to hypoxia and possibly with endothelial dysfunction.

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“…[2][3][4][5] Recently, using FMSF, we identified an unexpected effect of age on the vascular circulation and metabolic regulation in patients with type 1 diabetes (DM1). 6 The present contribution briefly describes the diagnostic potential of FMSF in the case of type 2 diabetes (DM2). Special attention is given to the ability of the FMSF technique to predict complications related to cardiovascular disease (CVD) associated with the progress of DM2.…”

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confidence: 99%

“…2-5 Recently, using FMSF, we identified an unexpected effect of age on the vascular circulation and metabolic regulation in patients with type 1 diabetes (DM1). 6…”

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confidence: 99%

Flow-Mediated Skin Fluorescence (FMSF) Technique for Studying Vascular Complications in Type 2 Diabetes

Katarzyńska

Borkowska

Łoś

et al. 2019

J Diabetes Sci Technol

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Flow Mediated Skin Fluorescence technique reveals remarkable effect of age on microcirculation and metabolic regulation in type 1 diabetes

Cited by 29 publications

References 20 publications

Can the microcirculatory response to hypoxia be a prognostic factor for Covid-19?

Can the microcirculatory response to hypoxia be a prognostic factor for Covid-19?

Changes of NADH Fluorescence from the Skin of Patients with Systemic Lupus Erythematosus

Flow-Mediated Skin Fluorescence (FMSF) Technique for Studying Vascular Complications in Type 2 Diabetes

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