Flower extract of Allium atroviolaceum triggered apoptosis, activated caspase-3 and down-regulated antiapoptotic Bcl-2 gene in HeLa cancer cell line

Khazaei, Sedigheh; Ramachandran, Vasudevan; Hamid, Roslida Abdul; Esa, Norhaizan Mohd; Etemad, Ali; Moradipoor, Sara; Ismail, Patimah

doi:10.1016/j.biopha.2017.02.082

Cited by 39 publications

(30 citation statements)

References 36 publications

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“…Methanolic flower extract of A. atroviolaceum (FAA) showed the ability to inhibit the growth of HeLa cells, both dose-and time-dependent. Importantly, the extract showed no toxicity toward normal 3T3 fibroblast, which demonstrates that FAA might be used as anticancer agent against cervix cancer [57]. The antiproliferative activity of Allium species seems to be related with the synergistic effect of various phytochemicals present in their bulbs of leaves, especially flavonoids and sulfur compounds.…”

Section: Antiproliferative Activity Of a Cornutum And A Cepa Extractsmentioning

confidence: 88%

“…Polyphenols showed to be able to induce the oxidative stress in cancer cells, leading to cell cycle disruption, DNA fragmentation, and finally apoptosis [73,74]. Numerous experiments suggested that organosulfur and phenolic compounds isolated from Allium species could induce apoptosis in cancer cells by the induction of p53 gene and activation of Caspase-3 [57,66,75]. The tumor-suppressor gene p53, which is often called "guardian Apoptosis is a programmed cell death that can be activated by different stimuli which induce DNA damage [71].…”

Section: Gene Expression Analysis In Hela Cellsmentioning

confidence: 99%

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Comparison of Organosulfur and Amino Acid Composition between Triploid Onion Allium cornutum Clementi ex Visiani, 1842, and Common Onion Allium cepa L., and Evidences for Antiproliferative Activity of Their Extracts

Fredotović

Soldo

Šprung

et al. 2020

Plants

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Species that belong to the genus Allium have been widely used for human food and traditional medicine. Their beneficial health effects, as well as the specific aroma, are associated with their bioactive chemical compounds, such as sulfur compounds and flavonoids. Gas chromatography and mass spectrometry (GC-MS) and reverse-phase high-performance liquid chromatography (reverse-phase HPLC) were used to identify organosulfur and amino acid content of triploid hybrid onion, Allium cornutum Clement ex Visiani, 1842, and common onion, Allium cepa L. Allium extracts were tested for their antiproliferative activity in three human cancer cell lines (HeLa, HCT116, and U2OS). DNA fragmentation and DAPI staining analysis were performed on HeLa cells to evaluate the effect of extracts on DNA damage and cell morphology. The mRNA expression of p53, Bax, and Caspase-3 genes involved in apoptosis were analyzed by real-time PCR. Using GC-MS, 27 compounds were found in two Allium species headspaces. Differences were noted among the main compound abundance in the headspace (although the major thiols and disulfides were qualitatively identic in both Allium species) and dipropyl disulfide, diisopropyl trisulfide, and (Z)-prop-1-enyl propyl trisulfide were predominant sulfides. Identification of amino acids and their quantities were determined by reverse-phase HPLC. Most abundant amino acids in both onions were arginine (Arg) and glutamic acid (Glu). The results of cytotoxicity testing confirmed antiproliferative effects of both species. The DNA fragmentation assay, DAPI staining and real time PCR analysis confirmed that A. cornutum and A. cepa extracts induced apoptosis in HeLa cells. This study presents the evidence for possible therapeutic use of A. cornutum and A. cepa extracts against human cervical carcinoma cell line.

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Section: Antiproliferative Activity Of a Cornutum And A Cepa Extractsmentioning

confidence: 88%

Section: Gene Expression Analysis In Hela Cellsmentioning

confidence: 99%

Comparison of Organosulfur and Amino Acid Composition between Triploid Onion Allium cornutum Clementi ex Visiani, 1842, and Common Onion Allium cepa L., and Evidences for Antiproliferative Activity of Their Extracts

Fredotović

Soldo

Šprung

et al. 2020

Plants

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show abstract

“…Caspases are a family of cysteine proteases with the functions of separated into initiator caspases of caspase‐9 and executioner caspases of caspase‐3. Initiator caspase‐9 is triggered in the occurrence of mitochondrial cytochrome‐ c leakage, which can activate caspase‐3 leading to apoptosis . Treatment with limonin resulted in dose‐dependent activation of caspase‐9, which simultaneously enhanced the caspase‐3 activity.…”

Section: Discussionmentioning

confidence: 99%

Anticancer effect of Limonin against benzo(a)pyrene‐induced lung carcinogenesis in Swiss albino mice and the inhibition of A549 cell proliferation through apoptotic pathway

Gong

Huo

et al. 2019

J Biochem & Molecular Tox

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The main purpose of the current study is to reveal the anticancer action of limonin against benzo(a)pyrene [B(a)P]‐treated lung carcinogenesis in Swiss albino mice and A549 lung cancer cells. B(a)P was orally supplemented (50 mg/kg body weight) twice a week for four weeks induction of lung cancer in mice. The lung weight, body weight, incidence of tumor, lipid peroxidation, carcinoembryonic antigen (CEA), enzymatic and nonenzymatic antioxidants (superoxide dismutase, GPx, glutathione, glutathione reductase, catalase, and glutathione S‐transferase), serum marker enzymes (aryl hydroxylase, lactate dehydrogenase, 5′‐nucleotidases, and γ‐glutamyl transpeptidase), and inflammatory mediators (interleukin‐1β, interleukin‐6, and tumor necrosis factor‐α) were estimated. Moreover, a histopathological study of lung tissues was supported by the biochemical analysis. Furthermore, the anticancer activity of limonin on A549 cells was measured by cell viability, production of reactive oxygen species (ROS), apoptotic morphological changes by AO/EtBr staining. Additionally, the status of apoptosis protein (caspase‐9 and ‐3) expressions was analyzed by the colorimetric analysis. B(a)P‐induced mice showed increased lipid peroxidation, CEA, serum marker enzymes and inflammatory cytokines levels with simultaneously decreased in the nonenzymatic and enzymatic antioxidants levels. Limonin supplements significantly reverted back to all these changes in this manner, showing the efficiency of anticancer effect. Furthermore, our in vitro study also supported the anticancer effect of the treatment of limonin‐enhanced apoptosis by loss of cell viability, improved ROS production, apoptotic morphological changes, and apoptosis protein expression were analyzed. Overall, these results suggest the anticancer potential of limonin against B(a)P‐induced lung cancer in Swiss albino mice and A549 lung cancer cells.

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“…1 The second most common gynecological tumors that caused mortality in women worldwide is cervical cancer. 2 Many studies assess some botanical products as the chemopreventive agent and more than 5000 bioactive compounds have been identified and still continuously increasing for the new one. These novel phytomedicines have shown running as anticancer drug candidate in vitro models.…”

Section: Introductionmentioning

confidence: 99%

Apoptosis of HeLa Cells via Caspase-3 Expression Induced by Chitosan-Based Nanoparticles of Annona squamosa Leaf Extract: In vitro Study

Fadholly¹,

Ansori²,

Proboningrat³

et al. 2020

IJPER

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Background: Annona squamosa is reported has a significant cytotoxic activity in some cancer cells. Objectives: Thus, this study aim to investigate Annona squamosa leaf extract induced by chitosan nanoparticles (nano-ASLE) to enhance their biological activity as anticancer agent on HeLa cells. Methods: Nano-ASLE (50, 100, 200, 400 µg/mL in DMSO) given on HeLa cells to determined IC 50 value by MTT assay. Then, it was devided into three groups as follow IC 50 , 2IC 50 , 4IC 50 continued with analysis of caspase-3 expression. Results: The present study demonstrated that nano-ASLE can surpress HeLa cells proliferation with the IC 50 value of 344.48 µg/mL and rapid enhancement of caspase-3 activity has the mean score of 65.3 cell expression and the lowest score shows 45.3 cell expression. Conclusion: Nano-ASLE lead to HeLa cell death via the mitochondrial pathway on caspase-3 expression. In addition, the further studies are needed to obtain the loading efficiency, release of drug concentration and in vivo study of nano-ASLE to suppress HeLa cells.

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Flower extract of Allium atroviolaceum triggered apoptosis, activated caspase-3 and down-regulated antiapoptotic Bcl-2 gene in HeLa cancer cell line

Cited by 39 publications

References 36 publications

Comparison of Organosulfur and Amino Acid Composition between Triploid Onion Allium cornutum Clementi ex Visiani, 1842, and Common Onion Allium cepa L., and Evidences for Antiproliferative Activity of Their Extracts

Comparison of Organosulfur and Amino Acid Composition between Triploid Onion Allium cornutum Clementi ex Visiani, 1842, and Common Onion Allium cepa L., and Evidences for Antiproliferative Activity of Their Extracts

Anticancer effect of Limonin against benzo(a)pyrene‐induced lung carcinogenesis in Swiss albino mice and the inhibition of A549 cell proliferation through apoptotic pathway

Apoptosis of HeLa Cells via Caspase-3 Expression Induced by Chitosan-Based Nanoparticles of Annona squamosa Leaf Extract: In vitro Study

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