Fluconazole Disk Diffusion Test with Methylene Blue- and Glucose-Enriched Mueller-Hinton Agar for Determining Susceptibility of
            <i>Candida</i>
            Species

Lee, Sai-Cheong; Fung, Chang-Phoné; Liao, Ning; See, Lai‐Chu; Huang, Jen‐Seng; Tsai, Chi-Jen; Chen, Kuo-Su; Shieh, Wen-Ben

doi:10.1128/jcm.39.4.1615-1617.2001

Cited by 40 publications

(32 citation statements)

References 15 publications

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“…In the studies of Pfaller M A et al, 14 and Lee S C et al, 15 it was seen that prepared MH-GMB agar plates provide acceptable performance for disk diffusion testing for at least 30 days when stored at 5°C.…”

Section: Discussionmentioning

confidence: 99%

Species distribution and in vitro antifungal susceptibility patterns of Candida

Mondal¹,

Mondal²,

Pal³

et al. 2013

Journal of Institute of Medicine

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Introduction: Due to advance in medical and surgical management nosocomial fungal infection rate has been increased. Recently Candididiasis has become one of the major fungal infections among hospitalized patients. Several Candida species can give rise to same clinical presentations but their antifungal susceptibility patterns may be different. The aim of the study is speciesLGHQWL¿FDWLRQ RI &DQGLGD LVRODWHG IURP GLIIHUHQW FOLQLFDO VDPSOHV DQG WR FDUU\ RXW WKHLU LQ YLWUR antifungal susceptibility tests.

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Section: Discussionmentioning

confidence: 99%

Species distribution and in vitro antifungal susceptibility patterns of Candida

Mondal¹,

Mondal²,

Pal³

et al. 2013

Journal of Institute of Medicine

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“…Twenty-six (86.6%) of the 30 MIC nonsusceptible strains were detected by the disk test on RPMIG and SHDM, and 27 (90%) were detected on MHGM. The agreement was lower when all three categories (susceptible, susceptible-dose dependent, and resistant) were used to analyze the data as has been observed by others (2,11,14,23).…”

mentioning

confidence: 87%

“…Recent studies (2,4,9,11,13,14,19; C. Durussel, A. M. Daoui-Hassani, and J. Bille, Abstr. 38th Intersci.…”

mentioning

confidence: 99%

Comparison of Results Obtained by Testing with Three Different Agar Media and by the NCCLS M27-A Method for In Vitro Testing of Fluconazole against Candida spp

et al. 2003

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Fluconazole susceptibilities of 150 Candida isolates were determined by a 25-g fluconazole disk diffusion agar test and compared with the microdilution NCCLS M27-A method. The agar test used three different media and was read at 24 and 48 h. When only the susceptible and nonsusceptible categories were used, disk diffusion with Müeller-Hinton agar supplemented with 2% glucose and 0.5 g of methylene blue (MHGM) per ml had a 95.37% correlation with the MIC method at 24 h, followed by RPMI 1640-2% of glucose agar (correlation, 94%) and Shadomy medium (SHDM) (correlation, 92.6%). The growth of microcolonies inside the inhibition zones was common (>63%) in the RPMI and SHDM media and minimal with MHGM (8.7%). At 48 h, MHGM and SHDM still had a >91% correlation with the MIC, while RPMI results had dropped to 75%. The best overall agreement was obtained with C. dubliniensis (100%).Fungal infection with Candida species is still an important cause of morbidity and mortality in immunocompromised patients. With the widespread use of fluconazole for the treatment and prevention of oropharyngeal and/or esophageal candidiasis, particularly in AIDS patients, clinical resistance is becoming a serious problem (5,7,8,12,21).What is needed is a rapid, easy, reproducible, and inexpensive in vitro method of obtaining susceptibility data which can guide the treatment of clinical yeast infections.The M27-A and M27-A2 reference NCCLS methods for antifungal susceptibility testing (15, 17) are cumbersome and costly, and reading the endpoints of the azoles is difficult. Alternative methods such as the broth microdilution adaptation of the M-27A and M27-A2 methods or the E test are simplified tests but are not easily adapted to the screening of yeasts for fluconazole susceptibility, nor are they cost-effective enough to be performed routinely in most clinical microbiology laboratories (6,18,20,24).Recent studies (2, 4, 9, 11, 13, 14, 19; C. Durussel, A. M. Daoui-Hassani, and J. Bille, Abstr. 38th Intersci. Conf. Antimicrob. Agents Chemother., abstr. J120, 1998) have demonstrated that an agar disk diffusion method similar to that used for testing antibacterial agents (NCCLS M2-A6) (16) can reproducibly and accurately determine the susceptibility of yeasts to fluconazole and can easily be incorporated into a clinical laboratory as an effective means for fluconazole susceptibility screening.The aim of this report is to study the agreement between the broth microdilution NCCLS method and a 25-g fluconazole disk diffusion test performed using RPMI 1640-2% glucose agar (RPMIG), Müeller-Hinton agar supplemented with 2% glucose and 0.5 g of methylene blue (MHGM) per ml, and Shadomy medium (SHDM).A total of 150 isolates of Candida spp. were collected from the clinical microbiology laboratory of Hospital Clínico Universitario Lozano Blesa (Zaragoza, Spain) and submitted for fluconazole susceptibility testing by microdilution and agar diffusion. The Candida species tested were 63 C. albicans isolates, 25 C. dubliniensis isolates, 25 C. glabrata isolat...

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“…Os resultados devem ser lidos após 24h. Esse método possui boa acurácia e é de baixo custo podendo ser acrescentado na rotina clínica laboratorial 6 .…”

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