Fluctuation and Localization of Endogenous Gibberellins in Rice

Kobayashi, Masatomo; Yamaguchi, Isomaro; Murofushi, Noboru; Ota, Yasuo; Takahashi, Noriko

doi:10.1080/00021369.1988.10868799

Cited by 37 publications

(49 citation statements)

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“…The observation that GAs are concentrated differentially in organs of both vegetative and reproductive Brassica plants is consistent with results from pea (Smith et al 1992), Silene , rice (Kobayashi et al 1988), and Avena (Kaufman et al 1976). In these other plants, concentrations of GAs were also found to be high in shoot tips, providing a consistent pattern of high GA concentration in these centers of growth and developmental activity.…”

Section: Endogenous Gas In Siliques and Seedssupporting

confidence: 78%

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Distribution of endogenous gibberellins in vegetative and reproductive organs of Brassica

Zanewich

Rood

1993

J Plant Growth Regul

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Recognizing the physiological diversity of different plant organs, studies were conducted to investigate the distribution of endogenous gibberellins (GAs) in Brassica (canola or oilseed rape). GA 1 and its biosynthetic precursors, GA20 and GA19, were extracted, chromatographically purified, and quantified by gas-chromatography-selected ion monitoring (GC-SIM), using [2H2]GAs as internal standards. In young (vegetative) B. napus cv. Westar plants, GA concentrations were lowest in the roots, increased acropetally along the shoot axis, and were highest in the shoot tips. GA concentrations were high but variable in leaves. GA~ concentrations also increased acropetally along the plant axis in reproductive plants. During early silique filling, GA1 concentrations were highest in siliques and progressively lower in flowers, inflorescence stalks (peduncles plus pedicels), stem, leaves, and roots.

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Section: Endogenous Gas In Siliques and Seedssupporting

confidence: 78%

“…GAg has been previously shown to be abundant in rice anthers (Kobayashi et al 1988) and maize tassels (Murofushi et al 1991), prompting the proposal that it may be involved in reproductive development. Further, Takahashi (1990) has presented evidence for the organ specificity of 13-OH versus non-13-OH GAs in rice.…”

Section: Endogenous Gas In Siliques and Seedsmentioning

confidence: 99%

Distribution of endogenous gibberellins in vegetative and reproductive organs of Brassica

Zanewich

Rood

1993

J Plant Growth Regul

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“…Quantitative analyses of GAs have been made in several species (e.g. Sponsel 1986;Fujioka et al 1988;Kobayashi et al 1988), but reliable quantitations of GAs in conifer tissues have not been reported.…”

Section: Introductionmentioning

confidence: 99%

Quantitation of gibberellins A1, A3, A4, A9 and an A9-conjugate in good- and poor-flowering clones of Sitka spruce (Picea sitchensis) during the period of flower-bud differentiation

Moritz

Philipson

Odén

1990

Planta

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The levels of endogenous gibberellin A1 (GA1), GA3, GA4, GA9 and a cellulase-hydrolysable GA9-conjugate in needles and shoot stems of Sitka spruce [Picea sitchensis (Bong.) Carr.] grafts with different coning or flowering histories were estimated by combined gas chromatography-mass spectrometry selected ion monitoring using deuterated GA3, GA4 and GA9 as internal standards. The samples were taken at the approximate time of the start of flower-bud differentiation, i.e. when the shoots had elongated approx. 95% of the final length. The needles of the good-flowering clones contained 11-12 ng per g fresh weight (FW) and 15-28 ng· (g FW) (-1) of GA9-conjugate and GA9, respectively. The shoot stems of the same material contained no detectable amounts of GA9-conjugate and 11-15 ng-(g FW)(-1) of GA9. The amounts of GA9-conjugate and GA9 were apparently lower in the poor-flowering clones, the needles containing 4-9 ng-(g FW)(-1) and 7-17 ng·(g FW)(-1), respectively. Also in this material the shoot stems contained no detectable amounts of GA9-conjugate. The amounts of GA4 were very small in both materials, ranging from 1-1.6 ng-(g FW)(-1). The good-flowering clones contained no detectable amounts of the more polar gibberellins, GA1 and GA3. The poor-flowering clones, on the other hand, contained high levels of GA15 17-19ng·(gFW)(-1) in the needles and 10-13 ng·(g FW) (-1) in the shoot stems, and also smaller amounts of GA3, 2-3 ng·(g FW)(-1) in the needles and approx. 1 ng·(g FW)(-1) in the shoot stems. The results demonstrate differences in GA-metabolism between the poor- and the good-flowering clones. The higher amounts of GA9-conjugate and GA9 might indicate a higher capacity for synthesizing GA4 in the good-flowering material. This synthesis does not, however, result in a build-up of the GA4-pool, maybe because of a high rate of turnover. Gibberellin A4 was apparently neither hydroxylated to GA1 nor converted to GA3 in the goodflowering material, as was the case in the poor-flowering material. This might indicate that gibberellin metabolism in the poor-flowering material is directed towards GA1 and GA3, GAs preferentially used in vegetative growth.

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“…The bioassay procedure was similar to the nondwarf rice seedling bioassay [12] except the seeds were treated with prohexadione calcium (BX-1 12, Calcium 3-hydroxy-4-propionyl-5-oxo-3-cyclohexenecarboxylate, Kumiai Chemical Co ., Ltd ., Taitoh [4] . Sites of inhibition by uniconazole [2], BX-112 [7,8] and dy gene [6] are indicated by single lines, double lines, and 'dy', respectively .…”

Section: Bioassay Proceduresmentioning

confidence: 99%

A highly-sensitive rice seedling bioassay for the detection of femtomole quantities of 3?-hydroxylated gibberellins

1993

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Fluctuation and Localization of Endogenous Gibberellins in Rice

Cited by 37 publications

References 0 publications

Distribution of endogenous gibberellins in vegetative and reproductive organs of Brassica

Distribution of endogenous gibberellins in vegetative and reproductive organs of Brassica

Quantitation of gibberellins A1, A3, A4, A9 and an A9-conjugate in good- and poor-flowering clones of Sitka spruce (Picea sitchensis) during the period of flower-bud differentiation

A highly-sensitive rice seedling bioassay for the detection of femtomole quantities of 3?-hydroxylated gibberellins

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