Fluorescence imaging in vivo visualizes delayed gastric emptying of liquid enteral nutrition containing pectin

Yamaoka, Ippei; Kikuchi, Takeshi; Endo, Naoyuki; Ebisu, Goro

doi:10.1186/1471-230x-14-168

Cited by 9 publications

(3 citation statements)

References 21 publications

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“…We also noticed that the CT-visualized volume of stomach decreased significantly after 8 h, in spite of increasing signals. Similar phenomenon was seen with silica-coated Bi 2 S 3 nanorods 81 . Although our corona complexes showed appreciable stability at pH 2, digestive enzymes in stomach are likely to decompose the corona shell and change crystalline structures of AuNPs and different crystal faces lead to different X-ray attenuations 82 .…”

Section: Resultssupporting

confidence: 76%

Probing the modulated formation of gold nanoparticles–beta-lactoglobulin corona complexes and their applications

et al. 2017

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Understanding interactions between proteins and nanoparticles (NPs) along with the underlying structural and dynamic information is of utmost importance to exploit nanotechnology for biomedical applications. Upon adsorption onto the NP surface, proteins form a well-organized layer that dictates the identity of the NP-protein complex named corona and governs its biological pathways. Given the high biological relevance, in-depth molecular investigations and applications of NPs-protein corona complexes are still scarce, especially since different proteins form unique patterns of corona and hence identification of biomolecular motifs at the interface is critical. In this work, we provide molecular insights and structural characterizations of the bionano interface of a popular food-based protein, bovine beta-lactoglobulin (β-LG), with gold nanoparticles (AuNPs) and the formation of corona complexes by combined molecular simulations and complementary experiments. Two major binding sites in β-LG were identified to be driven by citrate-mediated electrostatic interactions, while the associated binding kinetics and conformational changes in secondary structures were also characterized. More importantly, the superior stability of the corona led us to further explore its biomedical applications with examples of smart-phone based point-of-care biosensing of Escherichia coli (E. coli) and computed tomography (CT) of gastrointestinal (GI) tract through oral administration to probe GI tolerance and functions. Considering the biocompatibility, edible nature and efficient excretion through defecation, AuNPs-β-LG corona complexes have shown promising perspectives in future in vitro and in vivo clinical settings.

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Section: Resultssupporting

confidence: 76%

Probing the modulated formation of gold nanoparticles–beta-lactoglobulin corona complexes and their applications

et al. 2017

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“…We developed a liquid EN product containing LM pectin, in which the EN forms a gel under acidic conditions, as seen in the stomach (Yamaoka et al. ).…”

Section: Introductionmentioning

confidence: 99%

“…LM pectin forms a gel in the presence of divalent cations, while HM pectin gelates under conditions of high (≥50%) sugar content and low (<3.5) pH (Brejnholt 2010). We developed a liquid EN product containing LM pectin, in which the EN forms a gel under acidic conditions, as seen in the stomach (Yamaoka et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Fecal imaging demonstrates that low-methoxyl pectin supplementation normalizes gastro-intestinal transit in mice given a liquid diet

et al. 2018

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This study has the following aims: (1) to confirm a methodology for a fecal indocyanine green (ICG) imaging test for measuring gastro‐intestinal transit time (GITT); and (2) to compare GITT in mice given a liquid diet in which viscosity increases under acidic conditions to that in mice given stable liquid diets with comparable viscosity or regular chow. To address Aim 1, mice received ICG orally along with intraperitoneal injection of atropine in Study 1, and mice were given ICG orally with concurrent carmine red for Study 2. Fluorescence imaging of feces collected for 8 h thereafter was used to detect the first feces with fluorescence and thereby determine GITT. To address Aim 2, mice were fed ad libitum for 1 week with either liquid diet or regular chow for Study 3, or with liquid diet containing low‐methoxyl (LM) pectin or high‐methoxyl (HM) pectin, or regular chow for Study 4. GITT was then determined by fecal ICG imaging. Atropine delayed GITT in a dose‐dependent manner. The GITT of ICG completely corresponded to that of carmine red (correlation coefficient, 1.00). The first ICG excretion in the loose/some diarrheal feces of mice given a liquid diet was seen at 170 min. Feces of mice given liquid diet were loose with LM pectin and loose/some diarrhea with HM pectin. GITT of mice given liquid diet with HM pectin was significantly delayed (280 min) compared to that of mice given liquid diet with LM pectin (111 min) or regular chow (130 min). Fecal imaging of ICG enables measurements of GITT. LM pectin supplementation in a liquid diet may normalize GITT in mice to that of a normal meal and may be associated with changes in fecal properties.

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Noninvasive In Vivo Optical Imaging Models for Safety and Toxicity Testing

Peterson¹

2016

Nutraceuticals

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Fluorescence imaging in vivo visualizes delayed gastric emptying of liquid enteral nutrition containing pectin

Cited by 9 publications

References 21 publications

Probing the modulated formation of gold nanoparticles–beta-lactoglobulin corona complexes and their applications

Probing the modulated formation of gold nanoparticles–beta-lactoglobulin corona complexes and their applications

Fecal imaging demonstrates that low-methoxyl pectin supplementation normalizes gastro-intestinal transit in mice given a liquid diet

Noninvasive In Vivo Optical Imaging Models for Safety and Toxicity Testing

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