Fluorescence Polarization Immunoassay for Rapid, Accurate and Sensitive Determination of Ochratoxin A in Wheat

Lippolis, Vincenzo; Pascale, Michelangelo; Valenzano, Stefania; Porricelli, Anna Chiara Raffaella; Suman, Michele; Visconti, Angelo

doi:10.1007/s12161-013-9627-3

Cited by 28 publications

(20 citation statements)

References 27 publications

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“…These methods, which allow the detection of OTA alone or in combination with other mycotoxins, are mainly based on high-performance liquid chromatography (HPLC) coupled to fluorescence or mass spectrometry detection. Although most validated methods are chromatographic techniques, alternative strategies have been proposed; in particular, a number of promising immunochemical methods, such as enzyme-linked immunosorbent assays using monoclonal antibodies, fluorescence polarization immunoassays, lateral flow or immunochromatographic devices, flow-through immunoassays and optical or electrochemical biosensors including metal nanoparticles-based immunoassays have been reported for OTA analysis [10,11,12,13,14,15,16,17,18]. …”

Section: Introductionmentioning

confidence: 99%

Comparison of In-Solution Biorecognition Properties of Aptamers against Ochratoxin A

McKeague

Velu

Girolamo

et al. 2016

Toxins

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Ochratoxin A (OTA) is a mycotoxin produced as a secondary metabolite by several species of Aspergillus and Penicillium and frequently found as a natural contaminant in a wide range of food commodities. Novel and robust biorecognition agents for detecting this molecule are required. Aptamers are artificial nucleic acid ligands able to bind with high affinity and specificity to a given target molecule. In the last few years, three separate research groups have selected aptamers for ochratoxin A. While each of these three families of aptamers have been incorporated into various methods for detecting OTA, it is unclear if each aptamer candidate is better suited for a particular application. Here, we perform the first head-to-head comparison of solution-based binding parameters for these groups of aptamers. Based on our results, we provide recommendations for the appropriate choice of aptamer for incorporation into solution-based biorecognition assays and applications.

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Section: Introductionmentioning

confidence: 99%

Comparison of In-Solution Biorecognition Properties of Aptamers against Ochratoxin A

McKeague

Velu

Girolamo

et al. 2016

Toxins

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“…Colorimetric biosensing of OTA using gold nanoparticle has also been reported . Fluorescence polarization, with its simplicity, rapidity, cost‐effectiveness, and reliability, is a promising candidate as a screening technique to identify OTA contamination . Electrochemical methods based on adsorptive voltammetry have also been developed to render possible the simple and rapid determination of the presence of OTA .…”

Section: Introductionmentioning

confidence: 99%

Adsorptive Voltammetry for the Determination of Ochratoxin A Using Enrichment Effect by Cationic Surfactants

et al. 2018

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A rapid and simple electrochemical method was utilized for the determination of the presence of classic fungal metabolite ochratoxin A (OTA) based on differential pulse adsorptive voltammetry, using the carbon nanotube paste (CNTP) electrode. OTA showed a clear oxidation peak, and the CNTP electrode exhibited a more sensitive response to OTA oxidation than the glassy carbon, the plastic formed carbon, and the carbon paste electrodes. The addition of long‐chain cationic surfactants, such as benzethonium chloride, cetyltrimethylammonium bromide, and benzyldimethyltetradecylammonium chloride (zephiramine), enhanced the electrochemical response of OTA. Under optimized experimental conditions, the calibration plots are linear in the 5×10−6−5×10−5 M OTA concentration range, and the limit of detection is 1.1×10−6 M. In order to improve the selectivity for OTA determination, we attempted to combine this adsorptive stripping voltammetric technique with “sequestration electrochemistry,” based on the specific interaction occurring between bovine serum albumin (BSA) and OTA. The peak current of OTA oxidation decreased substantially as a result of OTA‐BSA binding. Furthermore, it was demonstrated that the application of this proposed method of hydrodynamic adsorptive voltammetry, using a micro‐droplet and a rotating disk electrode, improves the approach's sensitivity and reduces the time and sample volume required for the analysis.

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“…Fluorescence polarization (FP) assay is founded on the exceptional specificity of antibodies for their target antigens and has been extensively employed in clinical diagnostics . Nowadays, the receptor‐based FP assay has already been successfully applied to the detection of ochratoxin A , 17β‐estradiol , ractopamine , and phthalate esters . As a simple and rapid method, broad‐specific receptor‐based FP assay has several advantages, such as short assay time and absence of washing step in comparison with the enzyme‐linked immunosorbent assay.…”

Section: Introductionmentioning

confidence: 99%

Estrogen receptor‐based multi‐residue screening of bisphenol compounds in urine

Guan

Sun

et al. 2018

Biotech and App Biochem

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Human exposure to bisphenol compounds (BPs) has been implicated in the development of several chronic diseases. Instead of exploiting the traditional methods for determination of BPs, this work confirms that the human estrogen receptor α ligand binding domain (hERα-LBD) is a powerful recognition element that can be used to monitor multi-residue of BPs in urine samples by fluorescence polarization (FP) assay. Test parameters were optimized for the best performance. Under the optimal conditions, the IC 50 values of BPs are in the range of 0.04-1.61 μg mL −1 . Recovery experiments were then performed to assess the accuracy and precision of the established method. The results detected by FP assay show good agreements with that of liquid chromatography-tandem mass spectrometry method with a fit of R 2 = 0.9372 and 0.9640 for BPE and BPAP, respectively. A computational methodology, ligand-based pharmacophore model, was also employed to further explore the broad-specific of tested compounds. It was found that the two hydrogen bond acceptor features and one hydrophobic aliphatic feature were essential for the corresponding cross-reactivity results from the FP assay. All these results suggest that the established method can be successfully applied to monitor the occurrence

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Fluorescence Polarization Immunoassay for Rapid, Accurate and Sensitive Determination of Ochratoxin A in Wheat

Cited by 28 publications

References 27 publications

Comparison of In-Solution Biorecognition Properties of Aptamers against Ochratoxin A

Comparison of In-Solution Biorecognition Properties of Aptamers against Ochratoxin A

Adsorptive Voltammetry for the Determination of Ochratoxin A Using Enrichment Effect by Cationic Surfactants

Estrogen receptor‐based multi‐residue screening of bisphenol compounds in urine

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