Fluorescence polarization immunoassays for carbamazepine – comparison of tracers and formats

Abstract

Fast fluorescence polarization immunoassays for the detection of carbamazepine in surface water were developed.

Improved strategies for selection and characterization of new monoclonal anti-carbamazepine antibodies during the screening process using feces and fluorescence polarization immunoassay

“…The least sensitive measurement range is comparable to the previously developed CBZ FPIA using the same tracer, but a different antibody (test midpoint 13 mg L À1 , measurement range 1.5-310 mg L À1 ). 18 The upper limit of the measurement range also increased. This gives the opportunity to measure an even wider concentration range, once aer 10 min and if concentrations are too high at that moment, the MTP can be measured again aer 1 h.…”

“…The FP tracer CBZ-triglycine-5-(aminoacetamido)uorescein (CBZ-AAF) was previously synthesized. 18 CBZ-triglycine and the tracer for ELISA, CBZ-triglycine-horseradish peroxidase (CBZ-HRP) were previously prepared by Bahlmann et al 15 CBZ-triglycine-ovalbumin (CBZ-OVA) was prepared following the same procedure. 15 For the preparation of buffers and solutions, ultrapure water from a Milli-Q® Reference water purication system from Millipore was used.…”

“…15,16 FPIA for the determination of CBZ in serum is already used 17 and the application of this kind of assay to surface water samples has been recently described. 18 Previously described CBZ immunoassays were performed with a monoclonal anti-CBZ antibody, which showed high cross-reactivity (CR) against CBZ metabolites and related compounds, but also to the antihistaminic drug cetirizine which is not structurally close to CBZ. 19,20 This leads to overestimations of CBZ levels in water samples, especially during the hay fever season, when the antihistamine is present in waters.…”

Recent progress in nanomaterial-enhanced fluorescence polarization/anisotropy sensors

“…determination of various compounds, including pesticides [62], therapeutic drug [63][64][65][66], toxins or antibiotics [67][68][69][70][71][72][73], etc. [74][75][76], have been reported in literatures.…”

A fluorescence polarization immunoassay for detection of thiacloprid in environmental and agricultural samples

“…[18][19][20] Besides, the development of portable uorescence polarization detector makes FPIA more potential in pesticide residue detection. [21][22][23] In this work, we prepared an anti-thiacloprid mAb (mAb, C9) with high sensitivity, and labeled thiacloprid hapten with uorescein isothiocyanate (FITC) to develop FPIA for detection of thiacloprid. Under the optimal condition, the standard curve of FPIA for detection of thiacloprid was established.…”