Fluorescence Quenching of Quantum Dots by DNA Nucleotides and Amino Acids

Siegberg, Daniel; Herten, Dirk‐Peter

doi:10.1071/ch10293

Cited by 22 publications

(20 citation statements)

References 30 publications

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“…It is unlikely that the observed significant quenching of the PL was caused by aggregate formation, which usually causes spectral broadening or spectral shifts, since neither changes in the emission spectra nor shifts in the emission maxima of the QDs were observed (Figure 2b) in the presence of DNA 25, 26. Probably, electron transfer between QDs and nucleotide bases plays a major role, supported by the fact that DNA‐induced quenching was recently reported for cysteine‐capped QDs,11 which was attributed to electron transfer between QDs and nucleotide bases 11, 12. However, the exact mechanism of quenching is still elusive.…”

Section: Resultsmentioning

confidence: 82%

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A Quantum Dot Photoswitch for DNA Detection, Gene Transfection, and Live‐Cell Imaging

Eisele

Doroshenko

et al. 2012

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Quantum dots (QDs) coated with an albumin-derived copolymer shell exhibit significant photoresponsiveness to DNA loading and have great potential for investigating gene delivery processes. The QDs reported herein are positively charged, have attractive optical properties, and are noncytotoxic and notably stable in live cells. Their complex formation with plasmid DNA leads to proportionally decreased photoluminescence and efficient gene transfection is observed. Therefore, they are suitable for live-cell bioimaging and mechanistic studies of nonviral gene delivery. Fluorescence correlation spectroscopy is applied for the first time to investigate individual QDs diffusing in large endosomes inside living cells, and serves as a valuable tool to study the physical properties of QDs inside live cells. The data obtained in this study strongly support the notable stability of these QDs, even in cell endosomes.

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Section: Resultsmentioning

confidence: 82%

“…However, in such systems, labeling of nucleic acids is still necessary to investigate siRNA release. Recently, it has also been demonstrated that electron transfer between QDs and DNA induces QD emission quenching,11, 12 which is attractive for the development of label‐free DNA detection and delivery systems.…”

Section: Introductionmentioning

confidence: 99%

A Quantum Dot Photoswitch for DNA Detection, Gene Transfection, and Live‐Cell Imaging

Eisele

Doroshenko

et al. 2012

Small

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“…For this, we first assessed if the interaction with proteins will have any effect on the fluorescence properties of carbon dots. Interactions such as those with intracellular proteins have been observed to effect the fluorescence properties of various organic fluorophores and quantum dots . We monitored the fluorescence emission of carbon dots in Bovine serum albumin solution (4 mg/mL in phosphate buffer saline) at various pH over 12 hours.…”

Section: Methodsmentioning

confidence: 99%

Biocompatible Fluorescent Carbon Dots for Ratiometric Intracellular pH Sensing

Chandra

Singh

2017

ChemistrySelect

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We report here carbon dots (CDs) that can be utilized for intracellular pH sensing via fluorescence emission monitoring. The carbon dots are synthesized using a simple procedure from an economical carbon source Agaricus bisporus. Along with the excitation dependent fluorescence emission, typical to carbon dots, these CDs were found to have distinct emission maxima peaks at 450 nm and 550 nm when excited by 365 nm and 500 nm respectively. Interestingly, while the fluorescence emission at 450 nm was unaffected by pH changes in solution, the fluorescence emission intensity at 550 nm increased with decrease in pH, making these CDs a potential probe for pH sensing ratiometrically. Further, the CDs were found to be biocompatible and easily internalized in HeLa cells. We also demonstrated the utility of these CDs in sensing the changes in intracellular pH.

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“…concluded that nucleotides were not suitable stabilizers for these materials . A later study by Siegberg and Herten reported that nucleotide monophosphates quench quantum dot fluorescence upon adsorption, though stability studies were not performed …”

Section: Applications Of Nucleotide‐containing Cpsmentioning

confidence: 99%

Self‐Assembly of Nucleobase, Nucleoside and Nucleotide Coordination Polymers: From Synthesis to Applications

Lopez

Liu

2017

ChemNanoMat

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With multiple metal binding sites, nucleobases, nucleosides, and nucleotides form various coordination polymers (CPs) with metal ions. These self-assembled materials are very interesting for their simplicity to prepare, highly tunable structure and properties, and excellent biocompatibility.P olymerization is achieved under ambient conditions without the need of free radical initiators or UV light. Different nucleobases have different metal-binding preferences, but in general most of their coordinations ites prefer borderline or soft metals, while the phosphate groups in the nucleotides prefer hard Lewis acids. In this Focus Review,r ecent developments in this fielda re summarized startingf rom the synthesis and characterization of these CPs. Various metal ions includingg old, silver,l anthanides, zinc, copper, and iron are individually reviewed, and each metal brings its own property into the CP materials.While mostoft hese materials are non-crystalline nanoparticles, under certainc onditions crystalline metal-organic frameworks (MOFs) andh ydrogels can also be prepared. The applicationso fs uch CPs are then described including enzyme entrapment,d rug loading and delivery, biosensor development, catalysis, coating of nanoparticles, and luminescence. Finally,f uture research opportunities of this field are discussed.

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Fluorescence Quenching of Quantum Dots by DNA Nucleotides and Amino Acids

Cited by 22 publications

References 30 publications

A Quantum Dot Photoswitch for DNA Detection, Gene Transfection, and Live‐Cell Imaging

A Quantum Dot Photoswitch for DNA Detection, Gene Transfection, and Live‐Cell Imaging

Biocompatible Fluorescent Carbon Dots for Ratiometric Intracellular pH Sensing

Self‐Assembly of Nucleobase, Nucleoside and Nucleotide Coordination Polymers: From Synthesis to Applications

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