Fluorescent labelling of ciprofloxacin and norfloxacin and its application for residues analysis in surface water

Prutthiwanasan, Brompoj; Phechkrajang, Chutima; Suntornsuk, Leena

doi:10.1016/j.talanta.2016.05.080

Cited by 49 publications

(22 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For practical purposes, samples should be pretreated because their compositions may be complex and contain fluoroquinolone antibiotic residues in trace amounts. So far, techniques that have been used for the separation and enrichment of fluoroquinolone antibiotics (CIP, NRF and PEF) include molecularly imprinted polymer sorbent extraction, membrane‐based microextraction, solvent extraction and solid‐phase extraction . Although these techniques can decrease detection limits, they are not used in industrial settings due to their limitations (i.e.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous separation, concentration and determination of trace fluoroquinolone antibiotics in environmental samples using a polymer aqueous two‐phase system coupled with HPLC

Sun

Zhu

et al. 2019

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

BACKGROUND Fluoroquinolone antibiotics and their metabolites can accumulate in soil and water. Trace amounts of fluoroquinolone antibiotic residues in the environment necessitate the pretreatment of samples prior to analysis. In this work, a polyoxyethylene cetyl ether (POELE20)–NaH2PO4 aqueous two‐phase system (ATPS) was combined with high‐performance liquid chromatography (HPLC) to separate and determine trace quantities of ciprofloxacin (CIP), norfloxacin (NRF) and pefloxacin (PEF) in environmental samples. RESULTS Single‐factor, multi‐factor, reproducibility and accuracy experiments were performed. The extraction efficiencies and enrichment factors for the three antibiotics were above 90 and 13.8%, respectively, under optimal conditions. The recovery efficiency of CIP was 92.08–99.50%, with a relative standard deviation (RSD) of 0.60–6.34%, and the recovery efficiency of NRF and PEF was 93.40–99.30%, with RSD of 0.98–5.16%, when this method was applied to spiked water and soil samples. The RSDs of intra‐ and inter‐day analyses were 1.56–3.42 and 1.33–3.97%, respectively. CONCLUSIONS This study demonstrated that POELE20–NaH2PO4 ATPS coupled with HPLC was suitable for the simultaneous separation, concentration and determination of trace quantities of CIP, NRF and PEF in environmental samples. Moreover, this method can be applied for the determination of trace fluoroquinolone antibiotics in food samples. © 2019 Society of Chemical Industry

show abstract

Section: Introductionmentioning

confidence: 99%

Simultaneous separation, concentration and determination of trace fluoroquinolone antibiotics in environmental samples using a polymer aqueous two‐phase system coupled with HPLC

Sun

Zhu

et al. 2019

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

show abstract

“…The presence of FQs residues in effluents from households, hospitals, and pharmaceutical industries is a significant cause of acute and chronic toxicity, as well as the emergence of resistant bacteria. Consequently, removal of FQ residues from the environment is a crucial issue 15,16 . The structure and main physicochemical properties, as well as the concentrations of moxifloxacin and norfloxacin in different environmental compartments 5, 17-22 are listed in Table 1.…”

Section: Introductionmentioning

confidence: 99%

Adsorptive Removal Study of the Frequently Used Fluoroquinolone Antibiotics - Moxifloxacin and Norfloxacin from Wastewaters using Natural Zeolites

Rubashvılı

Eprikashvili²,

Kordzakhia³

et al. 2019

Mediterr.J.Chem.,

View full text Add to dashboard Cite

Residual antibiotics pollution has become one of the most severe environmental problems today.Â Antibiotics from hospitals and drug factories represent a potential risk for human and ecological health. Therefore, it has been a high exigency to develop some efficient and cost-effective treatment methods and technologies for antibiotics removal from industrial and household contaminated water. Adsorption is one of the most utilised techniques and has many competitive advantages such as the unique properties of flexibility, effectiveness, superior performance and the robustness for consecutive cycles. The present research concerns the adsorption of two frequently used fluoroquinolone antibiotics - moxifloxacin and norfloxacin on natural zeolite - clinoptilolite and its acid-modified form from aqueous solutions. For the first time, the adsorption of the antibiotics mentioned above on the selected natural zeolite was investigated under static and dynamic conditions. Adsorption experiment under dynamic conditions carried out using the specially constructed dynamic type of laboratory equipment. The effect of the inlet concentration, the flow rate and the pH value of the antibiotic solution, also, the contact time of system zeolite/antibiotic solution on the adsorption process were examined and evaluated using the Langmuir and Freundlich adsorption models. The results showed that the highest static adsorption capacities were observed at low initial concentration - 0.2 mg/mL of antibiotic solution for both adsorbents; the highest dynamic adsorption capacities - at low flow rate - 1.5 mg/mL and low inlet concentration - 0.2 mg/mL of antibiotic solution for both adsorbents. The static adsorption capacity was up to 2.71 mg/g for moxifloxacin hydrochloride; 4.14 mg/g for norfloxacin and the dynamic adsorption capacity was up to 1.20 mg/g for moxifloxacin hydrochloride; 2.10 mg/g for norfloxacin at a neutral pH value and constant temperature of 200°C. Each antibiotic was determined quantitatively in sample solutions using the developed and validated HPLC methods with a limit of quantitation - 0.05 Âµg/mL. Hence, this study demonstrates and proves that natural zeolite could be an effective adsorbent for the removal of the selected antibiotics from wastewaters.

show abstract

“…Fluorimetric-based methods have also been developed based on FQs intrinsic fluorescent properties as in the proposed methodology for quantification of CIP in seawater at ng L −1 level [23], or in the screening method applied in groundwater samples from intensive livestock production systems [24], or even resorting to automation for preconcentration in molecularly imprinted polymers [25]. Fluorescent labelling of FQs followed by chromatographic-fluorimetric detection has also been recently applied for the determination of CIP and NOR in surface water [26].…”

Section: Introductionmentioning

confidence: 99%

Screening of fluoroquinolones in environmental waters using disk-based solid-phase extraction combined to microplate fluorimetric determination and LC-MS/MS

Peixoto

Tóth

Barreiros

et al. 2019

International Journal of Environmental Analytical Chemistry

View full text Add to dashboard Cite

Fluoroquinolones are in the order of the day concerning environmental contamination through anthropogenic activities, resulting in increased risk for antibiotic resistance dissemination. In this context, accessible, low-cost analytical methods are required for implementation of comprehensive surveillance and screening schemes. In this work, we propose a down-scaled disk-based solidphase extraction system from which the eluate can be first screened by miniaturized fluorimetric reading, followed by individual determination of target fluoroquinolones (ciprofloxacin, norfloxacin, and enrofloxacin) by liquid chromatography combined to tandem mass spectrometry. The fluorimetric measurement is based on the intrinsic fluorescence of fluoroquinolones. Diskbased retention was performed after sample acidification (pH 4.0) by mixed-mode cation exchange using polystyrene divinylbenzene sulphonated sorbent. Sample loading was precisely controlled in a dedicated flow system operating at 4.0 mL min −1. Different eluent compositions were tested, with elution performed by 1.00 mL of methanol-ammonium hydroxide (98:2, v/v), with subsequent reading of eluate in both detectors. Quantification was attained for 2-25 µg L −1 range, with LOD values at 1 µg L −1. The proposed approach was successfully applied to estuarine waters from the Douro River, with comparable results to a conventional SPE-LC-MS/MS procedure.

show abstract

Fluorescent labelling of ciprofloxacin and norfloxacin and its application for residues analysis in surface water

Cited by 49 publications

References 26 publications

Simultaneous separation, concentration and determination of trace fluoroquinolone antibiotics in environmental samples using a polymer aqueous two‐phase system coupled with HPLC

Simultaneous separation, concentration and determination of trace fluoroquinolone antibiotics in environmental samples using a polymer aqueous two‐phase system coupled with HPLC

Adsorptive Removal Study of the Frequently Used Fluoroquinolone Antibiotics - Moxifloxacin and Norfloxacin from Wastewaters using Natural Zeolites

Screening of fluoroquinolones in environmental waters using disk-based solid-phase extraction combined to microplate fluorimetric determination and LC-MS/MS

Contact Info

Product

Resources

About