Fluorescent microscopy and Ziehl-Neelsen staining of bronchoalveolar lavage, bronchial washings, bronchoscopic brushing and post bronchoscopic sputum along with cytological examination in cases of suspected tuberculosis

Bodal, Vijay Kumar; Bal, Manjit Singh; Bhagat, Sunita D.; Kishan, Jai; Deepika,; Brar, Rupinder

doi:10.4103/0377-4929.168849

Cited by 16 publications

(13 citation statements)

References 11 publications

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“…6). This is in agreement with Bodal et al (2015) who reported that fluorescent staining was highly sensitive and can be used to confirm TB.…”

Section: Resultssupporting

confidence: 93%

Gross and histopathological lesions associated with tuberculosis in two sloth bears (Melursus ursinus) in India

Menon¹,

Bipin²,

Deepa³

et al. 2021

J Vet Anim Sci

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Post-mortem examination of two sloth bears which died in Bannerghatta Bear Rescue Centre, Bengaluru, Karnataka, were performed. Both the animals were anorectic and had considerable weight loss before death. Representative lung tissue samples were subjected to histopathology and staining. The lung tissues of the animals had diffuse congestion and subpleural petechial hemorrhages. In addition, small nodules of varied diameters were seen scattered on the lung lobes of both animals. On histopathological examination, the lung tissue of one of the animals showed extensive proliferation of blood vessels. Congestion and subpleural hemorrhages were seen in both cases. Few macrophages and epithelioid cells were seen scattered adjacent to a bronchiole. Kinyoun’s acid fast staining of the histological sections revealed numerous acid fast bacilli indicative of tuberculosis.

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“…6). This is in agreement with Bodal et al (2015) who reported that fluorescent staining was highly sensitive and can be used to confirm TB.…”

Section: Resultssupporting

confidence: 93%

Gross and histopathological lesions associated with tuberculosis in two sloth bears (Melursus ursinus) in India

Menon¹,

Bipin²,

Deepa³

et al. 2021

J Vet Anim Sci

View full text Add to dashboard Cite

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“…Gupta et al [8] Mitra et al [9] Paliwal et al [10] [11] observed similar results in their studies. Out of the total 52 cases, maximum number of cases 26 (50.00%) were seen in the 20-39 years age group and Bodal et al observed similar results in their study [12].…”

Section: Original Research Articlesupporting

confidence: 72%

Comparative Study of Ziehl-Neelsen Stain versus Fluorescent Microscopy in Diagnosis of Tuberculous Lymphadenitis on FNAC at A Tertiary Care Centre

Jadhav

Deshmukh

Mahato

et al. 2019

Trop J Pathol Microbiol

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Introduction: Worldwide, TB is one of the top 10 causes of death and the leading cause from a single infectious agent. Lymph nodes tuberculosis is considered the most common form of extrapulmonary tuberculosis and most common cause of lymphadenopathy in developing countries like India.Conventional Ziehl-Neelsen (ZN) method & Fluorescent microscopy (FM) plays an important role for detection of Acid fast bacilli (AFB). Objectives: This study is an attempt to find out cost effective, rapid and sensitive technique for early diagnosis of tuberculous lymphadenitis. To study incidence, age, sex and site wise distribution of tuberculous lymphadenitis in this area. Material Methods: The prospective observational study was carried out in the Department of Pathology, S.R.T.R. Govt. Medical College, Ambajogai in year 2017. All 247 aspirated samples from Lymph node swellings were subjected to ZN stain, Fluorescent stain, MGG & PAP stain and 52 cases of tuberculous lymphadenitis were subjected for further analysis, Results: Out of 247 samples aspirated from lymph node lesions 52 were of tuberculous lymphadenitis (21.05%). For tuberculous lymphadenitis, age ranged from 7 months to 77 years. Female predominance was noted with Female to male ratio 1.17:1. Half of the cases were in the range of 21-40 years of age. Cervical region was the commonest siteinvolved with 51.92%. Of 52 aspirates, smear positivity of AFB on ZN stain method was 78.84%, while positivity of Auramine fluorescent stain method was 90.38%. Conclusion: In developing countries with high prevalence of tuberculosis, Fine needle aspiration cytology (FNAC) coupled with fluorescent stain & ZN stain could distinctively improve diagnosis of tuberculous lymphadenitis in patients presenting with superficial lymphadenopathy.

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“…Davon sollen mindestens drei an unterschiedlichen Tagen gewonnene Proben untersucht werden. Falls ohnehin eine bronchoskopische Untersuchung durchgeführt wird, stellt die Entnahme von Sputum nach der Untersuchung eine wertvolle ergänzende diagnostische Möglichkeit dar [84,85].…”

Section: Präanalytikunclassified

S2k-Leitlinie: Tuberkulose im Erwachsenenalter

et al. 2017

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Since 2015 a significant increase in tuberculosis cases is notified in Germany, mostly due to rising numbers of migrants connected to the recent refugee crisis. Because of the low incidence in previous years, knowledge on tuberculosis is more and more limited to specialized centers. However, lung specialist and healthcare workers of other fields have contact to an increasing number of tuberculosis patients. In this situation, guidance for the management of standard therapy and especially for uncommon situations will be essential. This new guideline on tuberculosis in adults gives recommendations on diagnosis, treatment, prevention and prophylaxis. It provides a comprehensive overview over the current knowledge, adapted to the specific situation in Germany. The German Central Committee against Tuberculosis (DZK e. V.) realized this guideline on behalf of the German Respiratory Society (DGP). A specific guideline for tuberculosis in the pediatrics field will be published separately. Compared to the former recommendations of the year 2012, microbiological diagnostics and therapeutic drug management were given own sections. Chapters about the treatment of drug-resistant tuberculosis, tuberculosis in people living with HIV and pharmacological management were extended. This revised guideline aims to be a useful tool for practitioners and other health care providers to deal with the recent challenges of tuberculosis treatment in Germany.

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Fluorescent microscopy and Ziehl-Neelsen staining of bronchoalveolar lavage, bronchial washings, bronchoscopic brushing and post bronchoscopic sputum along with cytological examination in cases of suspected tuberculosis

Cited by 16 publications

References 11 publications

Gross and histopathological lesions associated with tuberculosis in two sloth bears (Melursus ursinus) in India

Gross and histopathological lesions associated with tuberculosis in two sloth bears (Melursus ursinus) in India

Comparative Study of Ziehl-Neelsen Stain versus Fluorescent Microscopy in Diagnosis of Tuberculous Lymphadenitis on FNAC at A Tertiary Care Centre

S2k-Leitlinie: Tuberkulose im Erwachsenenalter

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