Fluorescent on-site detection of multiple pathogens using smartphone-based portable device with paper-based isothermal amplification chip

Zhou, Qiaoqiao; Pan, Jiangfei; Mo, Lijun; Luo, Zhaofan; Qin, Zhifeng; Dai, Zong; Yi, Changqing

doi:10.1007/s00604-022-05419-x

Cited by 22 publications

(18 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… Zhou et al (2022) prepared a multi-chamber chip with a paper-disk and thin PDMS film for simultaneous detection of Escherichia coli O 157:H7, Salmonella spp., and Staphylococcus aureus . A portable device based on a smartphone was fabricated for end-point detection.…”

Section: Rapid On-chip Detection Methodsmentioning

confidence: 99%

“…As a result, there are two approaches used in fluorescent rapid detection methods: qualitative end-point detection with visual confirmation; and quantitative or semi-quantitative realtime detection with portable devices. Zhou et al (2022) prepared a multi-chamber chip with a paperdisk and thin PDMS film for simultaneous detection of Escherichia coli O 157:H7, Salmonella spp., and Staphylococcus aureus. A portable device based on a smartphone was fabricated for end-point detection.…”

Section: Fluorescent and Colorimetric Detectionmentioning

confidence: 99%

See 1 more Smart Citation

Rapid on-site nucleic acid testing: On-chip sample preparation, amplification, and detection, and their integration into all-in-one systems

Wang

Jiang

Pan

et al. 2023

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

As nucleic acid testing is playing a vital role in increasingly many research fields, the need for rapid on-site testing methods is also increasing. The test procedure often consists of three steps: Sample preparation, amplification, and detection. This review covers recent advances in on-chip methods for each of these three steps and explains the principles underlying related methods. The sample preparation process is further divided into cell lysis and nucleic acid purification, and methods for the integration of these two steps on a single chip are discussed. Under amplification, on-chip studies based on PCR and isothermal amplification are covered. Three isothermal amplification methods reported to have good resistance to PCR inhibitors are selected for discussion due to their potential for use in direct amplification. Chip designs and novel strategies employed to achieve rapid extraction/amplification with satisfactory efficiency are discussed. Four detection methods providing rapid responses (fluorescent, optical, and electrochemical detection methods, plus lateral flow assay) are evaluated for their potential in rapid on-site detection. In the final section, we discuss strategies to improve the speed of the entire procedure and to integrate all three steps onto a single chip; we also comment on recent advances, and on obstacles to reducing the cost of chip manufacture and achieving mass production. We conclude that future trends will focus on effective nucleic acid extraction via combined methods and direct amplification via isothermal methods.

show abstract

Section: Rapid On-chip Detection Methodsmentioning

confidence: 99%

Section: Fluorescent and Colorimetric Detectionmentioning

confidence: 99%

Rapid on-site nucleic acid testing: On-chip sample preparation, amplification, and detection, and their integration into all-in-one systems

Wang

Jiang

Pan

et al. 2023

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…The DMF-LAMP assay exhibited significantly enhanced color rendering and contrast for visual detection when compared with the real-time on-chip counterpart. Compared to other microfluidic-based colorimetric LAMP assays using fluorescent dyes, 20,[49][50][51] the proposed DMF-LAMP assay could be detected directly by the naked eye without the need for any electric detection system, which is more cost-effective and easier to implement.…”

Section: Optimization Of the Dmf-lamp Assaymentioning

confidence: 99%

A digital microfluidic platform coupled with colorimetric loop-mediated isothermal amplification for on-site visual diagnosis of multiple diseases

Xie

Chen²,

Cai

et al. 2023

Lab Chip

View full text Add to dashboard Cite

show abstract

“…As smartphones are emerging as the most promising monitoring devices, providing portability, affordability, and rapidity, they can be used as readout [ 107 , 108 , 109 , 110 ], image-processing [ 111 , 112 ], or operating tools [ 55 , 113 ] on diagnostic platforms. Furthermore, LAMP has been applied in diagnostic systems using smartphones owing to its various monitoring mechanisms, including color change, fluorescence, and turbidity, which can be implemented on smartphones.…”

Section: Devices To Monitor Lampmentioning

confidence: 99%

“…The device was designed for the real-time colorimetric monitoring of the LAMP reaction. Zhou et al developed paper fluidics on which LAMP amplification was enabled to detect Escherichia coli O157:H7 [ 113 ]. Smartphones can control the temperature of the module and collect fluorescent images.…”

Section: Devices To Monitor Lampmentioning

confidence: 99%

Principles and Applications of Loop-Mediated Isothermal Amplification to Point-of-Care Tests

Park

2022

Biosensors

View full text Add to dashboard Cite

For the identification of nucleic acids, which are important biomarkers of pathogen-mediated diseases and viruses, the gold standard for NA-based diagnostic applications is polymerase chain reaction (PCR). However, the requirements of PCR limit its application as a rapid point-of-care diagnostic technique. To address the challenges associated with regular PCR, many isothermal amplification methods have been developed to accurately detect NAs. Isothermal amplification methods enable NA amplification without changes in temperature with simple devices, as well as faster amplification times compared with regular PCR. Of the isothermal amplifications, loop-mediated isothermal amplification (LAMP) is the most studied because it amplifies NAs rapidly and specifically. This review describes the principles of LAMP, the methods used to monitor the process of LAMP, and examples of biosensors that detect the amplicons of LAMP. In addition, current trends in the application of LAMP to smartphones and self-diagnosis systems for point-of-care tests are also discussed.

show abstract

Fluorescent on-site detection of multiple pathogens using smartphone-based portable device with paper-based isothermal amplification chip

Cited by 22 publications

References 43 publications

Rapid on-site nucleic acid testing: On-chip sample preparation, amplification, and detection, and their integration into all-in-one systems

Rapid on-site nucleic acid testing: On-chip sample preparation, amplification, and detection, and their integration into all-in-one systems

A digital microfluidic platform coupled with colorimetric loop-mediated isothermal amplification for on-site visual diagnosis of multiple diseases

Principles and Applications of Loop-Mediated Isothermal Amplification to Point-of-Care Tests

Contact Info

Product

Resources

About