Fluorimetric and ratiometric colorimetric dual-mode detection of organophosphorus pesticides based on carbon dots/DTNB

Feng, Yaoyao; Qu, Yujiao; Sun, Xiaobo; Pan, Wei; Wang, Jinping

doi:10.1039/d1nj06011b

Cited by 9 publications

(2 citation statements)

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“…To verify the accuracy of the proposed method, the detection results of our method for AChE activity determination were compared with the typical colorimetric method (DTNB) for AChE activity (Table S6). The colorimetric principle is based on the hydrolysis of ACh catalyzed by AChE to produce choline, which reacts with 5,5-dithiobis(2-nitrobenzoic acid) (DTNB) to produce 5-thio-2-nitrobenzoic acid (TNB), which has an obvious absorption peak at 420 nm. , The absorbance was recorded to calculate the AChE activity, as shown in Figure S15. The results of the proposed method are consistent with those of the typical colorimetric method, and Figure indicates that there is no significant difference for the found values between the two assay protocols ( P > 0.05).…”

Section: Resultsmentioning

confidence: 99%

Manipulating the Photocatalytic Activity of Eu-Coordinated Hydrogen-Bonded Organic Framework Nanorods Decorated With Au Nanoclusters for the Multiplexed Luminescence Assay of Acetylcholinesterase

Li,

Wen,

Chen

et al. 2024

ACS Appl. Nano Mater.

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An integration of multiplexed luminescence and photocatalysis assay was proposed for the detection of acetylcholinesterase (AChE) by manipulating the coordination immobilization of photocatalytic Au nanoclusters on Eu-HOF nanorods. Eu-HOF-AuNCs served as a luminescence indicator and mimicked the enzyme toolbox for universally generating reactive oxygen species (ROS). Under light irradiation, Eu-HOF-AuNCs may accelerate the catalytic oxidation of oPD to produce more oligomers with yellow fluorescence and in situ-regulate photoluminescence variations of the indicator, in which oligomers can weaken the phosphorescence of the indicator based on overlapped spectra. By utilizing thiocholine (enzymolysis product of acetylthiocholine) to preferentially compete for ROS produced by photocatalysis of Eu-HOF-AuNCs, AChE activity was detected by the reversal of the indicator signal due to the attenuation of oPD oligomers. The emerged ratiometric fluorescence F 573 /F 340 and F 617 /F 340 exhibit improved sensitivity with detection limits of AChE as low as 0.09 and 0.14 U/L, respectively. Also, the cooperation of phosphorescence of Eu-HOF-AuNCs boosts detection accuracy. Serum experiments show that the Eu-HOF-AuNCs can be used to determine AChE, promoting the possibility of application in disease diagnosis. This sensor driven by photocatalysis has a great potential to be developed into a universal oxidase platform for quantifying analytes as long as the reasonable redox reaction.

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Section: Resultsmentioning

confidence: 99%

Manipulating the Photocatalytic Activity of Eu-Coordinated Hydrogen-Bonded Organic Framework Nanorods Decorated With Au Nanoclusters for the Multiplexed Luminescence Assay of Acetylcholinesterase

Li,

Wen,

Chen

et al. 2024

ACS Appl. Nano Mater.

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“…Carbon dots with 5,5-dithiobis-(2-nitrobenzoic acid) were used for fluorometric and colorimetric dual detection method for detecting organophosphorus pesticides. The method was highly sensitive, selective and accurate [41].…”

Section: Applications Of Cnds In Detection Of Poisonsmentioning

confidence: 96%

Potential forensic applications of carbon nanodots

Sowmyya

2023

J. Phys.: Conf. Ser.

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Nanotechnology is a flourishing branch of research due to the incredible properties nanomaterials exhibit over their massive counterparts. Carbon nanodots are the members of the carbon nanoparticle family with prominent properties like hydrophilicity, low toxicity, biocompatibility, increased stability and ease of functionalization. They are excellent replacement to metal based quantum dots which are highly toxic. Carbon nanodots are being used in the fields of medicine, environmental science, drug delivery and forensic science. Applications of carbon nanodots can be seen in bio imaging, bio sensing, cancer diagnosis and treatment, biological activities, solar cells and as photo catalysts. Forensic science is the science behind crime investigation and evidence analysis. Development of novel, rapid, on site testing materials for accurate, specific and sensitive detection of forensic evidences is required for delivery of justice to the victims of crime. Carbon nanodots have shown promising applications in the field of forensic science as well. Use of carbon nanodots as invisible ink for anti-counterfeiting application of currency and security documents and use of these nanomaterials in development of latent fingerprints are some forensic applications. Carbon nanodots based detection of food contaminants, heavy metal poisons, pesticides, illicit drugs and explosives are other forensic applications. Carbon nanodots are showing a future direction in development of on field testing kits for forensic evidences.

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An ultra-sensitive photoelectrochemical sensor for chlorpyrifos detection based on a novel BiOI/TiO2 n-n heterojunction

Lyu

Lei

Zhang

et al. 2023

Analytica Chimica Acta

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Fluorimetric and ratiometric colorimetric dual-mode detection of organophosphorus pesticides based on carbon dots/DTNB

Abstract: In this work, a fluorimetric and ratiometric colorimetric dual-mode detection method was developed for organophosphorus pesticides (OPs) based on carbon dots (CDs)/5,5-dithiobis-(2-nitrobenzoic acid) (DTNB). Its main mechanism is that OPs...

Cited by 9 publications

References 51 publications

Manipulating the Photocatalytic Activity of Eu-Coordinated Hydrogen-Bonded Organic Framework Nanorods Decorated With Au Nanoclusters for the Multiplexed Luminescence Assay of Acetylcholinesterase

Manipulating the Photocatalytic Activity of Eu-Coordinated Hydrogen-Bonded Organic Framework Nanorods Decorated With Au Nanoclusters for the Multiplexed Luminescence Assay of Acetylcholinesterase

Potential forensic applications of carbon nanodots

An ultra-sensitive photoelectrochemical sensor for chlorpyrifos detection based on a novel BiOI/TiO2 n-n heterojunction

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