Fluorimetric Determination of Plasma Cortisol in Chronic Renal Failure

A continuous-flow method for the determination of cortisone, hydrocortisone, prednisolone and methylprednisolone in the range 0.100-1.00 pg ml-1 and of dexamethasone and betamethasone in the range 0.500-5.00 pg ml-1 is described. The procedure is based on the action of each corticosteroid as a sensitiser on the chemiluminescent oxidation of sulphite by cerium(lV). The analysis is automated, requires no sample pre-treatment and samples can be analysed at a rate of 40 per hour with a relative error of <6%. The method was evaluated by carrying out recovery experiments and by analysing commercial formulations. Results agreed well with those given by an official method.

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Determination of Hydrocortisone in Pharmaceutical Drug by TLC With Densitometric Detection in Uv

Pyka

Babuška

Bocheńska

2011

Journal of Liquid Chromatography & Related Technologies

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Fluorimetric Determination of Plasma Cortisol in Chronic Renal Failure

Cited by 4 publications

References 11 publications

Chemiluminescent flow injection determination of alkaline phosphatase and its applications to enzyme immunoassays

Chemiluminescent flow injection determination of alkaline phosphatase and its applications to enzyme immunoassays

Continuous-flow chemiluminescence determination of some corticosteroids

Determination of Hydrocortisone in Pharmaceutical Drug by TLC With Densitometric Detection in Uv

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