Fluoxetine-induced up-regulation of 14-3-3zeta and tryptophan hydroxylase levels in RBL-2H3 cells

Lipopolysaccharide-induced serotonin transporter up-regulation involves PKG-I and p38MAPK activation partially through A3 adenosine receptor

“…Many studies have demonstrated RBL-2H3 cells expressing serotonin synthesis and transporter systems as an ideal model for elucidating the regulatory mechanisms relating to SERT (14)(15)(16). Consistent with previous reports, results obtained in our laboratory have shown that the fluorescent styryl dye ASP assay enables the detection of dynamic transport activities of SERT and is amenable for measuring 5-HT uptake (17)(18)(19)(20)(21).The results obtained from the present study showed that LPS treatment enhances 5-HT uptake in RBL-2H3 cells in a dosedependent manner. LPS-induced up-regulation of 5-HT uptake results from the increase in surface-expressed SERT protein, which might be partially mediated by PKG-I and p38MAPK activation following A3 adenosine receptor activation.…”

Effect of fluoxetine on the expression of tryptophan hydroxylase and 14-3-3 protein in the dorsal raphe nucleus and hippocampus of rat

“…Therefore, based on our results and the data from Duman et al (2000), it would be valuable to study whether 14-3-3 protein plays a specific role in the neuroprotective functions of fluoxetine. Recently, some studies observed an association between fluoxetine and 14-3-3 protein (Baik et al, 2005;Cecconi et al, 2007). In a study by Baik et al (2005), it was reported that the mRNA and protein levels of 14-3-3 z, one of the seven known isoforms, was upregulated after fluoxetine treatment for 2 and 3 days in rat basophilic RBL-2H3 cells, which have an active 5-HT system.…”

Glutamate-related gene expression changes with age in the mouse auditory midbrain

“…The threshold cycle (C T ) values, defined as the number of PCR cycles taken to reach the greatest amplification level, were measured to detect the threshold of each of the five significant and GAPDH genes in all IC samples (Giulietti et al, 2001;Baik et al, 2005). Each sample was measured in triplicate and normalized to the reference GAPDH gene expression.…”