Foliar and Tuber Symptoms of U.S. Potato Varieties to Multiple Strains and Isolates of Potato virus Y

Whitworth, Jonathan L.; Gray, Stewart M.; Ingram, Jason T.; Hall, Darren G.

doi:10.1007/s12230-020-09820-1

Cited by 10 publications

(2 citation statements)

References 23 publications

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“…In terms of morphological outcomes, the types, onset, and progression of PVY-induced foliar symptoms occurred as described previously ( Goyer et al., 2015 ; Whitworth et al., 2021 ), albeit in a strain- and cultivar-dependent manner. In Premier Russet, for example, inoculations with PVY O and PVY N-Wi developed HR, while inoculations with PVY NTN or mock control did not lead to HR ( Supplementary Figure S3 ).…”

Section: Discussionmentioning

confidence: 61%

Pathogen-triggered metabolic adjustments to potato virus Y infection in potato

et al. 2023

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Potato (Solanum tuberosum L) is affected by several viral pathogens with the most economically damaging being potato virus Y (PVY). At least nine biologically distinct variants of PVY are known to attack potato, with necrotic types named PVYNTN and PVYN-Wi being the most recent additions to the list. So far, the molecular plant-virus interactions underlying this pathogenicity are not fully understood. In this study, gas chromatography coupled with mass spectroscopy (GC–MS) was used for an untargeted investigation of the changes in leaf metabolomes of PVY-resistant cultivar Premier Russet, and a susceptible cultivar, Russet Burbank, following inoculation with three PVY strains, PVYNTN, PVYN-Wi, and PVYO. Analysis of the resulting GC–MS spectra with the online software Metaboanalyst (version 5.0) uncovered several common and strain-specific metabolites that are induced by PVY inoculation. In Premier Russet, the major overlap in differential accumulation was found between PVYN-Wi and PVYO. However, the 14 significant pathways occurred solely due to PVYN-Wi. In contrast, the main overlap in differential metabolite profiles and pathways in Russet Burbank was between PVYNTN and PVYO. Overall, limited overlap was observed between PVYNTN and PVYN-Wi. As a result, PVYN-Wi-induced necrosis may be mechanistically distinguishable from that of PVYNTN. Furthermore, 10 common and seven cultivar-specific metabolites as potential indicators of PVY infection and susceptibility/resistance were identified by using PLS-DA and ANOVA. In Russet Burbank, glucose-6-phosphate and fructose-6-phosphate were particularly affected by strain–time interaction. This highlights the relevance of the regulation of carbohydrate metabolism for defense against PVY. Some strain- and cultivar-dependent metabolite changes were also observed, reflecting the known genetic resistance–susceptibility dichotomy between the two cultivars. Consequently, engineering broad-spectrum resistance may be the most effective breeding strategy for managing these necrotic strains of PVY.

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Section: Discussionmentioning

confidence: 61%

Pathogen-triggered metabolic adjustments to potato virus Y infection in potato

et al. 2023

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“…PVY has many strains, having different phenotypic characters and resistance patterns, which are mainly host-dependent ( Whitworth et al., 2021 ). But there is still a lack of a complete mechanistic understanding of the roles of different autophagy-related host factors in controlling virus infection.…”

Section: Introductionmentioning

confidence: 99%

P3/P3N-PIPO of PVY interacting with BI-1 inhibits the degradation of NIb by ATG6 to facilitate virus replication in N. benthamiana

et al. 2023

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IntroductionAutophagy not only plays an antiviral role but also can be utilized by viruses to facilitate virus infection. However, the underlying mechanism of potato virus Y (PVY) infection against plant autophagy remains unclear. BI-1, localizing to the endoplasmic reticulum (ER), is a multifunctional protein and may affect the virus infection.MethodsIn this study, Y2H, BiFC, qRT-PCR, RNA-Seq, WB and so on were used for research.ResultsP3 and P3N-PIPO of PVY can interact with the Bax inhibitor 1 (BI-1) of N. benthamiana. However, BI-1 knockout mutant showed better growth and development ability. In addition, when the BI-1 gene was knocked out or knocked down in N. benthamiana, the PVY-infected mutant showed milder symptoms and lower virus accumulation. Analysis of transcriptome data showed that the deletion of NbBI-1 weakened the gene expression regulation induced by PVY infection and NbBI-1 may reduce the mRNA level of NbATG6 by regulated IRE1-dependent decay (RIDD) in PVY-infected N. benthamiana. The expression level of the ATG6 gene of PVY-infected WT was significantly down-regulated, relative to the PVY-infected mutant. Further results showed that ATG6 of N. benthamiana can degrade NIb, the RNA-dependent RNA polymerase (RdRp) of PVY. NbATG6 has a higher mRNA level in PVY-infected BI-1 knockout mutants than in PVY-infected WT.ConclussionThe interaction of P3 and/or P3N-PIPO of PVY with BI-1 decrease the expression of the ATG6 gene might be mediated by RIDD, which inhibits the degradation of viral NIb and enhances viral replication.

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Overexpression of VQ Motif-Containing Gene does not Affect Infection Rates of Potato with Potato Virus Y

Goyer

Bvindi

2023

Am. J. Potato Res.

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Foliar and Tuber Symptoms of U.S. Potato Varieties to Multiple Strains and Isolates of Potato virus Y

Cited by 10 publications

References 23 publications

Pathogen-triggered metabolic adjustments to potato virus Y infection in potato

Pathogen-triggered metabolic adjustments to potato virus Y infection in potato

P3/P3N-PIPO of PVY interacting with BI-1 inhibits the degradation of NIb by ATG6 to facilitate virus replication in N. benthamiana

Overexpression of VQ Motif-Containing Gene does not Affect Infection Rates of Potato with Potato Virus Y

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