Follicle-stimulating hormone signaling and Foxl2 are involved in transcriptional regulation of aromatase gene during gonadal sex differentiation in Japanese flounder, Paralichthys olivaceus

Yamaguchi, Toshiya; Yamaguchi, Sakiko; Hirai, Toshiaki; Kitano, Takeshi

doi:10.1016/j.bbrc.2007.05.208

Cited by 217 publications

(126 citation statements)

References 26 publications

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“…This idea is well supported by results in fishes and birds showing that estrogens can modulate foxl2 gonadal expression (Baron et al 2004, Hudson et al 2005, Wang et al 2007, which is known as one of the few important ovarian determining genes in vertebrates (Baron et al 2004, 2005a, Govoroun et al 2004, Ottolenghi et al 2005, Nakamoto et al 2006, Pannetier et al 2006, Wang et al 2007). This estrogen modulation of foxl2 and the recent demonstration that FOXL2/foxl2 positively regulates Cyp19/cyp19a1 expression (Pannetier et al 2006, Wang et al 2007, Yamaguchi et al 2007, also provided strong support to the hypothesis that foxl2 and estrogens act within a short positive feedback loop (Hudson et al 2005, Vizziano et al 2008. Apart from the early down-regulation of female genes, the masculinization process also involved some up-regulations of Sertoli cells (i.e.…”

Section: Introductionsupporting

confidence: 54%

“…In the gilthead seabream (Sparus aurata), FSHB is expressed in primary-and secondary-growth oocytes and this pituitary hormone may be involved in communication between oocytes and ovarian follicle cells (Wong & Zohar 2004). Interestingly, in the Japanese flounder (Paralichthys olivaceus), both FSH signaling and FOXL2 have been shown to be involved in the transcriptional regulation of CYP19A1 during gonadal differentiation (Yamaguchi et al 2007).…”

Section: Discussionmentioning

confidence: 99%

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Estrogen treatment up-regulates female genes but does not suppress all early testicular markers during rainbow trout male-to-female gonadal transdifferentiation

Vizziano-Cantonnet¹,

Báron²,

Mahé³

et al. 2008

Journal of Molecular Endocrinology

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In non-mammalian vertebrates, estrogens are key players in ovarian differentiation, but the mechanisms by which they act remain poorly understood. The present study on rainbow trout was designed to investigate whether estrogens trigger the female pathway by activating a group of early female genes (i.e. cyp19a1, foxl2a, foxl2b, fst, bmp4, and fshb) and by repressing early testicular markers (i.e. dmrt1, nr0b1, sox9a1 and sox9a2). Feminization was induced in genetically allmale populations using 17a-ethynylestradiol (EE2, 20 mg/kg of food during 2 months). The expression profiles of 100 candidate genes were obtained by real-time RT-PCR and 45 expression profiles displayed a significant differential expression between control populations (males and females) and EE2-treated populations. These expression profiles were grouped in five temporally correlated expression clusters. The estrogen treatment induced most of the early ovarian differentiation genes (foxl2a, foxl2b, fst, bmp4, and fshb) and in particular foxl2a, which was strongly and quickly up-regulated. Simultaneously, Leydig cell genes, involved in androgen synthesis, as well as some Sertoli cell markers (amh, sox9a2) were strongly repressed. However, in contrast to our initial hypothesis, some genes considered as essential for mammalian and fish testis differentiation were not suppressed during the early process of estrogen-induced feminization (dmrt1, nr0b1, sox9a1 and pax2a) and some were even strongly up-regulated (nr0b1, sox9a1and pax2a). In conclusion, estrogens trigger male-to-female transdifferentiation by up-regulating most ovarian specific genes and this up-regulation appears to be crucial for an effective feminization, but estrogens do not concomitantly down-regulate all the testicular differentiation markers.

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Section: Introductionsupporting

confidence: 54%

Section: Discussionmentioning

confidence: 99%

Estrogen treatment up-regulates female genes but does not suppress all early testicular markers during rainbow trout male-to-female gonadal transdifferentiation

Vizziano-Cantonnet¹,

Báron²,

Mahé³

et al. 2008

Journal of Molecular Endocrinology

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“…This difference represents just a fraction (8%) of the ∼15,000 DETs that were differentially expressed between untreated females (FCT) and males (MCT), which, in turn, account for a significant part of the estimated number of protein-coding genes (>25,000) in zebrafish. In fish, masculinization by elevated temperature usually involves down-regulation of profemale genes, such as cyp19a1a and foxl2, and up-regulation of promale genes, such as dmrt1 or amh (8)(9)(10)(11)(12)(13)(14). In accordance with this general pattern, the FHT2 females with a male-like gonadal transcriptome exhibited down-regulation of both key profemale genes such as cyp19a1a and vtg5 as well as downregulation of profemale pathways such as oocyte meiosis, progesterone-mediated oocyte maturation, canonical Wnt signaling (28,53), and Fanconi anemia (26).…”

Section: Discussionmentioning

confidence: 99%

“…The effects of temperature on gene expression during sex differentiation have been investigated in different teleost species, including the African catfish, Clarias gariepinus (8), European sea bass, Dicentrarchus labrax (9, 10), Japanese flounder, Paralichthys olivaceus (11), pejerrey, Odontesthes bonariensis (12), and Nile tilapia, Oreochromis niloticus (13,14). Regardless of the actual underlying sex-determining mechanism, a shared characteristic of all fish species in which temperature can alter sex ratios is that exposure to heat during early development upregulates the expression of genes related to testis differentiation with a concomitant down-regulation of genes related to ovarian differentiation, as assessed in several species (e.g., 9,13,[15][16][17].…”

mentioning

confidence: 99%

Heat-induced masculinization in domesticated zebrafish is family-specific and yields a set of different gonadal transcriptomes

Ribas

Liew

Díaz

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

119

136

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Understanding environmental influences on sex ratios is important for the study of the evolution of sex-determining mechanisms and for evaluating the effects of global warming and chemical pollution. Fishes exhibit sexual plasticity, but the underlying mechanisms of environmental effects on their reproduction are unclear even in the well-established teleost research model, the zebrafish. Here we established the conditions to study the effects of elevated temperature on zebrafish sex. We showed that sex ratio response to elevated temperature is family-specific and typically leads to masculinization (female-to-male sex reversal), resulting in neomales. These results uncovered genotype-by-environment interactions that support a polygenic sex determination system in domesticated (laboratory) zebrafish. We found that some heat-treated fish had gene expression profiles similar to untreated controls of the same sex, indicating that they were resistant to thermal effects. Further, most neomales had gonadal transcriptomes similar to that of regular males. Strikingly, we discovered heat-treated females that displayed a normal ovarian phenotype but with a "male-like" gonadal transcriptome. Such major transcriptomic reprogramming with preserved organ structure has never been reported. Juveniles were also found to have a male-like transcriptome shortly after exposure to heat. These findings were validated by analyzing the expression of genes and signaling pathways associated with sex differentiation. Our results revealed a lasting thermal effect on zebrafish gonads, suggesting new avenues for detection of functional consequences of elevated temperature in natural fish populations in a global warming scenario.A major paradox in developmental biology is the lack of conservation of sex-determining mechanisms even in closely related taxa (1). Thus, in mammals and birds, sex is genetically canalized by a chromosomal sex determination (CSD) system of male (XX/XY in mammals) or female (ZW/ZZ in birds) heterogamety, driven by the action of a master sex-determining gene: sry in mammals and dmrt1 in birds (1). In contrast, fish sex can be very plastic, with the combination of genetic and environmental influences (2). In addition to fish species with CSD, there are species with polygenic sex determination (PSD), in which sex depends on the combined action of several promale and profemale genetic factors, and species with temperature-dependent sex determination (TSD) (3). Understanding how the environment influences sex ratios in vertebrates is of fundamental importance in the study of the evolution of sex-determining mechanisms (4, 5). It is also of practical relevance in evaluating the effects of climate change and chemical pollution (6, 7).The effects of temperature on gene expression during sex differentiation have been investigated in different teleost species, including the African catfish, Clarias gariepinus (8), European sea bass, Dicentrarchus labrax (9, 10), Japanese flounder, Paralichthys olivaceus (11), pejerrey, Odontesthes...

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“…Abundant literature exists on broodstock management and culture technology of other flatfishes of the genus Paralichthys such as P. olivaceus in Japan (Alam et al, 2002;Furuita et al, 2002;Hernández et al, 2007;Yamaguchi et al, 2007), P. microps in Chile (Silva, 2001), P. californicus (Conklin et al, 2003;Gisbert et al, 2004;Merino et al, 2007;Palumbo et al, 2007) and P. lethostigma (Smith et al, 1999;Watanabe & Carroll, 1999) in the United States, P. orbignyanus in Argentina and Brazil Müller et al, 2006;Lanes et al, 2009Lanes et al, , 2010; although little is known about the use of Recirculating Aquaculture System (RAS) technology for broodstock culture of P. adspersus.…”

Section: Introductionmentioning

confidence: 99%

Broodstock management of the fine flounder Paralichthys adspersus (Steindachner, 1867) using recirculating aquaculture systems

Carrera

Cota

Montes

et al. 2013

lajar

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ABSTRACT. The present study describes the methodology used at IMARPE for the capture, acclimation and management of P. adspersus broodstock using recirculating aquaculture systems (RAS). RAS improved the water quality and maintained the environmental parameters during the acclimation period, temperature (17.2±1ºC), oxygen (8.1±0.7 mg L -1 ), pH (7.3±0.2), ammonia (0.004±0.003 mg L -1 ), nitrite (0.52±0.2 mg L -1 ) and nitrate (3.45±2.6 mg L -1 ). Fish began to be fed normally from day 15 post-capture, once or twice a day using live fish (Odonthestes regia regia, Mugil cephalus), crustacean (Emerita analoga), fresh food (Engraulis ringens and Dosidicus gigas) and artificial feed. A significant loss in the weight of the fish was registered during the first days of captivity, followed by a continuous increase in both sexes. The specific growth rate was positive from the third month of captivity, being the relative growth rate 24.5% and 16.2% in August 2010 in males and females, respectively. Different internal and external parasites were detected in the fish, being Entobdella sp. and Philometra sp. the prevailing parasites observed during samplings. Keywords: Paralichthys adspersus, reproduction, recirculating aquaculture system, broodstock management, culture, Peru. Manejo de reproductores del lenguado Paralichthys adspersus (Steindachner, 1867)usando sistemas de recirculación en acuicultura RESUMEN. El presente trabajo, describe la metodología desarrollada en IMARPE para la captura, aclimatación y acondicionamiento de ejemplares adultos de P. adspersus en sistemas de recirculación (SRA), con la finalidad de formar un stock de reproductores. El SRA permitió manejar parámetros medioambientales estables durante el periodo de acondicionamiento, como: temperatura del agua (17,2±1ºC), oxígeno disuelto (8,1±0,7 mg L -1 ), pH (7,3±0,2), amonio (0,004±0,003 mg L -1 ), nitrito (0,52±0,2 mg L -1 ) y nitrato (3,45±2,6 mg L -1 ). Se dio inicio a la alimentación el día 15 post-captura, utilizando alimento vivo (Odonthestes regia regia, Mugil cephalus), crustáceos (Emerita analoga), alimento fresco (Engraulis ringens y Dosidicus gigas) y artificial semihúmedo. Durante los primeros días de acondicionamiento los peces mostraron una disminución en el peso, hasta su adaptación a las condiciones de cultivo, luego de lo cual se produjo un incremento continuo en ambos sexos. La tasa específica de crecimiento fue positiva a partir del tercer mes y la tasa de crecimiento relativo mostró que en agosto 2010, el peso promedio se incrementó 24,5% en machos y 16,2% en hembras. Se realizó un análisis patológico a los ejemplares capturados y se observó la presencia de diferentes parásitos internos y externos, entre ellos predominaron Entobdella sp. y Philometra sp. Latin American Journal of Aquatic ResearchPalabras clave: Paralichthys adspersus, reproducción, sistema de recirculación en acuicultura, manejo de reproductores, cultivo, Perú.

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Follicle-stimulating hormone signaling and Foxl2 are involved in transcriptional regulation of aromatase gene during gonadal sex differentiation in Japanese flounder, Paralichthys olivaceus

Cited by 217 publications

References 26 publications

Estrogen treatment up-regulates female genes but does not suppress all early testicular markers during rainbow trout male-to-female gonadal transdifferentiation

Estrogen treatment up-regulates female genes but does not suppress all early testicular markers during rainbow trout male-to-female gonadal transdifferentiation

Heat-induced masculinization in domesticated zebrafish is family-specific and yields a set of different gonadal transcriptomes

Broodstock management of the fine flounder Paralichthys adspersus (Steindachner, 1867) using recirculating aquaculture systems

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