Fontibacillus panacisegetis sp. nov., isolated from soil of a ginseng field

Abstract

A facultatively anaerobic, endospore-forming bacterium, designated strain P11-6 T , was isolated from soil of a ginseng field located in Geumsan County, Republic of Korea. Cells of strain P11-6 T were Gram-stain-negative, catalase-negative, motile rods and produced semi-translucent, circular, white colonies on tryptic soy agar. The isolate contained MK-7 as the only menaquinone and anteiso-C 15 : 0 as the major fatty acid. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unknown aminophosphoglycolipid, an unknown aminophospholipid, two unknown phospholipids, three unknown glycolipids and three unknown lipids were detected in the polar lipid profile. The DNA G+C content of strain P11-6 T was 41.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequencing showed that strain P11-6 T was most closely related to Fontibacillus aquaticus GPTSA 19 T (97.2 % sequence similarity) and that it formed a separate lineage with F. aquaticus in the family Paenibacillaceae. Combined phenotypic and DNA-DNA hybridization data supported the conclusion that strain P11-6 T represents a novel species in the genus Fontibacillus, for which the name Fontibacillus panacisegetis sp. nov. is proposed; the type strain is P11-6 T (5KCTC 13564The family Paenibacillaceae of the order Bacillales currently comprises nine recognized genera, Ammoniphilus, Aneurinibacillus, Brevibacillus, Cohnella, Fontibacillus, Oxalophagus, Paenibacillus, Saccharibacillus and Thermobacillus, which accommodate Gram-stain-positive, -variable or -negative, sporulating bacilli. The genus Paenibacillus (type species, Paenibacillus polymyxa) was created by Ash et al. (1993) to accommodate the so-called rRNA group 3 bacilli comprising 11 Bacillus species on the basis of comparative 16S rRNA gene sequence analysis. Since then, ten more Bacillus species and one Clostridium species have been transferred to this genus and a huge number of novel species have been described. Thus, at the time of writing, the genus Paenibacillus contained 109 recognized species (Euzéby, 2010). Most recently, the genus Fontibacillus has been described on the basis of its 16S rRNA gene dissimilarity with respect to related species of the genus Paenibacillus and its unique polar lipid profile, despite its phylogenetic position within the large Paenibacillus cluster (Saha et al., 2010).Strain P11-6 T was recovered during the course of an investigation of the culturable aerobic and facultatively anaerobic bacterial community in soil from a ginseng field. At first, the strain was considered to belong to the genus Paenibacillus on the basis of preliminary 16S rRNA gene sequence comparison, although it showed low levels of similarity (,96 %) to all recognized species of this genus. However, after the description of the novel genus Fontibacillus, strain P11-6 T was found to show a higher 16S rRNA gene sequence similarity (.97 %) to Fontibacillus aquaticus GPTSA 19 T , the only member of this genus. Thus, a polyphasic taxonomic investigation was carried out to clarify the taxo...

Fontibacillus pullulanilyticussp.nov. isolated from soil

“…The DNA G+C content of DSHK107 T was 42.1 mol%, a value consistent with that of members of the genus Fontibacillus .…”

“…The predominant isoprenoid quinone found in strain DSHK107 T , menaquinone‐7 (MK‐7), was the same as that found in recognized members of the genus Fontibacillus .…”

“…The fatty acid composition of strain DSHK107 T and related Fontibacillus species were given in Table . The major fatty acid of strain DSHK107 T was anteiso‐C 15:0 , which is also the major fatty acids in all members of the genus Fontibacillus . The ratio of fatty acids iso‐C 15:0 , iso‐C 16:00 , and C 16:00 was relatively high compared with the related strains of the genus Fontibacillus .…”

“…The identified lipids of strain DSHK107 T , F. aquaticus DSM 17643 T , F. solani A4STR04 T , and F. panacisegetis DSM 28129 T were similar. Regarding the unknown polar lipids, one unknown lipid (UL10), one unknown aminolipid (AL4), one unknown phospholipid (PL13), and one unknown aminophospholipid (PN3) were present only in strain DSHK107 T .…”

“…Members of this genus are heterotrophic, facultatively anaerobic and rod‐shaped, motile, and possess menaquinone‐7 (MK‐7) as the predominant respiratory quinone . At the time of writing, this genus comprised four species with validly published names (http://www.bacterio.cict.fr/) and members have been isolated from soil of a warm spring, soil, Phaseolus vulgaris nodules and potato root .…”