Food allergy to shrimps and fish in patients suffering from atopic dermatitis, the results of ISAC Multiplex examination

Čelakovská, Jarmila; Bukač, Josef; Vaňková, R.; Krejsek, Jan; Andrýs, Ctirad; Krčmová, Irena

doi:10.1080/09540105.2020.1826911

Cited by 6 publications

(2 citation statements)

References 53 publications

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“…We report here the most likely allergens forming the underlying molecular basis for this not well-understood clinical phenomenon. Furthermore, the current study, for the first time, identified allergens that are possibly responsible for clinical cross-reactivity between shrimp and fish [25]. Beta-enolase and aldolase A, enzymatic proteins of the glycolytic pathway, were identified as heat labile allergens in various fish species and chicken [15,76,77].…”

Section: Discussionmentioning

confidence: 74%

“…These two allergens were not included in the main results of this study as they were identified from plant and fungi species (Triticum aestivum and Aspergillus fumigatus) that are phylogenetically distant to shrimps. However, there is emerging evidence of patients suffering from shrimp allergy also being sensitised to plant and fungi allergens [25,26]. The allergen cyclophilin is generally found in dust mite, fungi and plants, and demonstrates strong IgE-binding [63,78].…”

Section: Discussionmentioning

confidence: 99%

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Novel Allergen Discovery through Comprehensive De Novo Transcriptomic Analyses of Five Shrimp Species

Karnaneedi

Huerlimann

Johnston

et al. 2020

IJMS

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Shellfish allergy affects 2% of the world’s population and persists for life in most patients. The diagnosis of shellfish allergy, in particular shrimp, is challenging due to the similarity of allergenic proteins from other invertebrates. Despite the clinical importance of immunological cross-reactivity among shellfish species and between allergenic invertebrates such as dust mites, the underlying molecular basis is not well understood. Here we mine the complete transcriptome of five frequently consumed shrimp species to identify and compare allergens with all known allergen sources. The transcriptomes were assembled de novo, using Trinity, from raw RNA-Seq data of the whiteleg shrimp (Litopenaeus vannamei), black tiger shrimp (Penaeus monodon), banana shrimp (Fenneropenaeus merguiensis), king shrimp (Melicertus latisulcatus), and endeavour shrimp (Metapenaeus endeavouri). BLAST searching using the two major allergen databases, WHO/IUIS Allergen Nomenclature and AllergenOnline, successfully identified all seven known crustacean allergens. The analyses revealed up to 39 unreported allergens in the different shrimp species, including heat shock protein (HSP), alpha-tubulin, chymotrypsin, cyclophilin, beta-enolase, aldolase A, and glyceraldehyde-3-phosphate dehydrogenase (G3PD). Multiple sequence alignment (Clustal Omega) demonstrated high homology with allergens from other invertebrates including mites and cockroaches. This first transcriptomic analyses of allergens in a major food source provides a valuable resource for investigating shellfish allergens, comparing invertebrate allergens and future development of improved diagnostics for food allergy.

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Section: Discussionmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 99%

Novel Allergen Discovery through Comprehensive De Novo Transcriptomic Analyses of Five Shrimp Species

Karnaneedi

Huerlimann

Johnston

et al. 2020

IJMS

View full text Add to dashboard Cite

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The relation between the sensitization to molecular components of inhalant allergens and food reactions in patients suffering from atopic dermatitis

Čelakovská

Bukač

Vaňková

et al. 2021

Food and Agricultural Immunology

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To evaluate the relation between the sensitization to molecular components of inhalant allergens and the occurrence of food reactions in atopic dermatitis patients. Another aim is to evaluate the semi-quantification results of the ISAC Multiplex testing to reveal the relevance of the leading allergens. The complete dermatological and allergological examination including the examination of the sensitization to molecular components with Multiplex ISAC testing was performed. The food reactions to examined food allergens were confirmed in the open exposure test (history). Altogether 100 atopic dermatitis patients were examined -48 men, 52 women, the average age 40.9 years. The leading molecular components are cysteine protease (Der f 1, Der p 1), NPC2 protease (Der f 2, Der p 2), Acidic glycoprotein (Alt a 1), PR 10 proteins (Bet v 1, Mal d 1, Aln g 1 and Cor 1.04), lipocalins (Can f 1, Fel d 4, Mus m 1, Equ c 1), uteroglobulin (Fel d 1), Kallikrein (Can f 5) and molecular components of Timothy and Bermuda grass (glycoprotein). The significantly higher occurrence of sensitization to molecular components Aln g 1, Bet v 1, Phl p 1, 2, 4, 5, 6, 11 was confirmed in patients suffering from reactions to peanuts, hazelnuts, celery, apple and peach.

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Food allergy to apple, peach and celery in atopic dermatitis patients, analysis of sensitisation to molecular components

Čelakovská

Bukač

Vaňková

et al. 2021

Food and Agricultural Immunology

Self Cite

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Food allergy to shrimps and fish in patients suffering from atopic dermatitis, the results of ISAC Multiplex examination

Cited by 6 publications

References 53 publications

Novel Allergen Discovery through Comprehensive De Novo Transcriptomic Analyses of Five Shrimp Species

Novel Allergen Discovery through Comprehensive De Novo Transcriptomic Analyses of Five Shrimp Species

The relation between the sensitization to molecular components of inhalant allergens and food reactions in patients suffering from atopic dermatitis

Food allergy to apple, peach and celery in atopic dermatitis patients, analysis of sensitisation to molecular components

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