Forensic efficacy of twelve STRs in Spanish cattle

Gamarra, David; López-Oceja, A.; Jiménez-Moreno, Susana; Pancorbo, Marian M. de

doi:10.1016/j.fsigss.2015.09.101

Cited by 3 publications

(9 citation statements)

References 2 publications

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“…Genetic relationships of Pirenaica were analyzed against Terreña, Salers and Holstein-Friesian STR data from our previous studies [15,16]. Reynolds genetic distance measures were computed by Arlequin 3.5 [22].…”

Section: Discussionmentioning

confidence: 99%

“…Assignment tests were performed including several breeds typically raised in the Basque region. On one hand, minority PGI breeds such as native Terreña [15], Blonde d´Aquitaine and Limousin [11] were included using published STR data. On the other hand, Salers and Holstein-Friesian breeds, genotyped in this study using the 30-STR panel, were included.…”

Section: Discussionmentioning

confidence: 99%

“…In Pirenaica, the PIC values of most loci exceeded 0.6, except for HEL13 (0.421), INRA035 (0.384) and ILST005 (0.302). Based on heterozygosity and PIC values, Pirenaica had slightly lower genetic diversity than other Basque breeds such as Terreña, Monchina and Betizu [14,15]. According to Mendizabal et al [43], the Pirenaica population increased significantly during the 1850s, but the later introgression of new cattle breeds from Europe lead to an endangered situation of the former breed.…”

Section: Genetic Variations and Genetic Relationshipsmentioning

confidence: 99%

“…In this study, considering the 30 STRs panel and a cutoff value of 80% (Q ≥ 0.8), the overall proportion of animals correctly assigned to a breed was 98.8%. However, assignment tests were performed using a 11 STR panel and Basque native Terreña genetic data [15]; Pirenaica assignment decreased to 66.1%, whereas 31% of Pirenaica animals were uncorrected assigned to Terreña breed. In contrast, the Terreña, Salers and Holstein-Friesian showed higher assignments, 85.3, 94.8 and 96.6%, respectively.…”

Section: Assignment Analysis Of Breedsmentioning

confidence: 99%

“…In the Pirenaica breed, a 10-STR panel was studied for traceability purposes [ 12 ] and other few and/or not core STRs were reported for genetic diversity [ 13 , 14 ]. In other breeds included in the Basque PGI label, such as autochthonous Terreña, or imported Blonde d´Aquitaine, Limousin and Salers, genetic diversity studies have been reported [ 15 , 16 , 17 ]. However, most of previous studies were performed before the recommendation by the FAO for using a 30-STR panel, and therefore, limitations of markers make for a difficult the comparison with data from later studies [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

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Genetic Characterization of the Local Pirenaica Cattle for Parentage and Traceability Purposes

Gamarra

Taniguchi

Aldai

et al. 2020

Animals

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Pirenaica is the most important autochthonous cattle breed within the Protected Geographic Indication (PGI) beef quality label in the Basque region, in northern Spain. The short tandem repeats (STRs) are powerful markers to elucidate forensic cases and traceability across the agri-food sector. The main objective of the present work was to study the phylogenetic relationships of Pirenaica cattle and other breeds typically raised in the region and provide the minimum number of STR markers for parentage and traceability purposes. The 30-STR panel recommended by the International Society of Animal Genetics-Food and Agriculture Organization of the United Nations (ISAG-FAO) was compared against other commercial STR panels. The 30-STR panel showed a combined matching probability of 1.89 × 10−25 and a power of exclusion for duos of 0.99998. However, commercial STR panels showed a limited efficiency for a reliable parentage analysis in Pirenaica, and at least a 21-STR panel is needed to reach a power of exclusion of 0.9999. Machine-learning analysis also demonstrated a 95% accuracy in assignments selecting the markers with the highest FST in Pirenaica individuals. Overall, the present study shows the genetic characterization of Pirenaica and its phylogeny compared with other breeds typically raised in the Basque region. Finally, a 21-STR panel with the highest FST markers is proposed for a confident parentage analysis and high traceability.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Genetic Variations and Genetic Relationshipsmentioning

confidence: 99%

Section: Assignment Analysis Of Breedsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Genetic Characterization of the Local Pirenaica Cattle for Parentage and Traceability Purposes

Gamarra

Taniguchi

Aldai

et al. 2020

Animals

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Forensic parameters for eleven STRs in Angus-Brangus breed from Northwest Colombia

Palacio

Ibarra

Posada

et al. 2017

Forensic Science International: Genetics Supplement Series

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Livestock (Bos taurus) is one of the main sources of protein in the human diet, and is one of the major commercial activities worldwide involving billions of dollars annually; Molecular genetics has been involved for decades in the study of this species. In the present work, 339 bovines of the Angus-Brangus breed were STR typified, allelic frequencies and forensic parameters were calculated, and Hardy-Weimberg equilibrium was assesed.

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Selection and use of microsatellite markers for individual identification and meat traceability of six swine breeds in the Chinese market

Zhao

Zhu

et al. 2017

Food sci. technol. int.

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Meat traceability based on molecular markers is exerting a great influence on food safety and will enhance its key role in the future. This study aimed to investigate and verify the polymorphism of 23 microsatellite markers and select the most suitable markers for individual identification and meat traceability of six swine breeds in the Chinese market. The mean polymorphism information content value of these 23 loci was 0.7851, and each locus exhibited high polymorphism in the pooled population. There were 10 loci showing good polymorphism in each breed, namely, Sw632, S0155, Sw2406, Sw830, Sw2525, Sw72, Sw2448, Sw911, Sw122 and CGA. When six highly polymorphic loci were combined, the match probability value for two random individual genotypes among the pig breeds (Beijing Black, Sanyuan and Taihu) was lower than 1.151 E-06. An increasing number of loci indicated a gradually decreasing match probability value and therefore enhanced traceability accuracy. The validation results of tracing 18 blood and corresponding meat samples based on five highly polymorphic loci (Sw2525, S0005, Sw0107, Sw911 and Sw857) were successful, with 100% conformation probability, which provided a foundation for establishing a traceability system for pork in the Chinese market.

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Forensic efficacy of twelve STRs in Spanish cattle

Cited by 3 publications

References 2 publications

Genetic Characterization of the Local Pirenaica Cattle for Parentage and Traceability Purposes

Genetic Characterization of the Local Pirenaica Cattle for Parentage and Traceability Purposes

Forensic parameters for eleven STRs in Angus-Brangus breed from Northwest Colombia

Selection and use of microsatellite markers for individual identification and meat traceability of six swine breeds in the Chinese market

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