Formation of a Tight 1:1 Complex of Clostridium pasteurianum Fe Protein−Azotobacter vinelandii MoFe Protein:  Evidence for Long-Range Interactions between the Fe Protein Binding Sites during Catalytic Hydrogen Evolution

Abstract

It has been well documented that the combination of the MoFe protein of Azotobacter vinelandii nitrogenase (Av1) with the Fe protein (Cp2) from Clostridium pasteurianum nitrogenase produces an inactive, stable complex. However, we report that this heterologous nitrogenase has a low level of activity for H(2) evolution, with a specific activity of 12 nmol min(-)(1) mg(-)(1) of Av1. This activity does not arise from contaminating hydrogenase since it required the presence of both Cp2 and Av1 and showed saturation kinetics when increasing amounts of Cp2 were added to the assay. Incubation of the two proteins at a 4:1 Cp2:Av1 ratio in the absence of MgATP followed by analytical gel filtration showed, surprisingly, that the stoichiometry of the isolated complex was Av1.Cp2 instead of Av1.(Cp2)(2) as determined previously. The presence of MgATP in the elution buffer did not change the elution profile of the complex. The hydrodynamic radius of the isolated complex determined by dynamic light scattering was 5.93 +/- 0.14 nm, intermediate between Av1 and a stable 2:1 nitrogenase complex, consistent with a 1:1 assignment for the Av1.Cp2 complex. When assayed with Av2, the isolated Av1.Cp2 complex showed full half-site reactivity with a specific activity of 750 nmol of C(2)H(2) reduced min(-)(1) mg(-)(1) of Av1. The EPR spectrum of the isolated complex showed the Cp2 to be oxidized and the Av1 to retain the S = (3)/(2) signal characteristic of FeMoco. In the presence of MgATP, under turnover conditions at a 2:1 ratio of Cp2:Av1, the [4Fe-4S] center of Cp2 was protected from the chelator 2,2'-bipyridyl. This is consistent with the formation of a tight 2:1 complex of Av1.(Cp2)(2) which is more stable than the homologous Cp nitrogenase. Assuming that the Lowe-Thorneley model for nitrogenase applies and that a rate-limiting dissociation of the complex is required for H(2) evolution, then with a rate of 0.032 s(-)(1) the 1:1 complex is too stable to be involved in catalysis. The differences in the stability of the 2:1 and 1:1 complexes indicate cooperativity between the Fe protein binding sites of Av1, which structural data show to be separated by 105 A. On the basis of these observations, we propose a model for nitrogenase catalysis in which the stable 1:1 complex formed between oxidized Fe protein and the one-electron-reduced MoFe protein plays an essential role. In this scheme, the two Fe protein binding sites of the MoFe protein alternately bind and release Fe protein in a shuttle mechanism associated with long-range conformational changes in the MoFe protein.

Clostridium pasteurianum W5 synthesizes two NifH-related polypeptides under nitrogen-fixing conditions

“…Studies on heterologous pairs showed that an incompatible Fe protein could act as an inhibitor of nitrogenase activity (Emerich & Burris, 1978;Clarke et al, 2000).…”

Evidence for the Selective Population of FeMo Cofactor Sites in MoFe Protein and Its Molecular Recognition by the Fe Protein in Transition State Complex Analogues of Nitrogenase

“…view of the recent evidence for long range conformational changes in the MoFe protein upon Fe protein binding (26).…”

“…An alternative, in which MgATP binds rapidly to a complex of Fe and MoFe proteins, followed by subsequent conformational changes in the Fe protein, has been proposed (24). Moreover, in the heterologous nitrogenase formed between the Fe protein of C. pasteurianum and the MoFe protein from A. vinelandii, MgATP is not required for complex formation (25,26).…”

Long-range interactions between the Fe protein binding sites of the MoFe protein of nitrogenase

“…With Av1, Cp2 forms tight 1:1 and 2:1 Cp2 . Av1 complexes, which are only marginally active in reducing protons and cannot reduce other substrates [19,20,21,22]. In contrast, Cp2 .…”