Formation of ion-conducting channels by the membrane attack complex proteins of complement

Shiver, John W.; Dankert, J.; Esser, Alfred F.

doi:10.1016/s0006-3495(91)82110-5

Cited by 19 publications

(8 citation statements)

References 31 publications

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“…In contrast to the pores in the N18 neuroblastoma cells, those in the bovine red blood cells might be larger, with at least some with an r h > 3.78 nm. This observation and the statistically significant difference between the asymmetric conductance distributions in the N18 neuroblastoma cells and the CHO‐K1 cells strongly suggest that: (i) the OlyA/PlyB pores are composed of a varied number of oligomerised units, as has been indicated previously [4] and reported for MACPF/CDC proteins [15–24] – this is also consistent with our observation that the Renkin's model of the osmoticants transport, assuming a population of equally sized pores [9,11], could not be applied – and (ii) the size of the pores vary with the membrane composition, as reported previously for some pore‐forming proteins [24,25].…”

Section: Discussionsupporting

confidence: 91%

“…In contrast to the pores in the N18 neuroblastoma cells, those in the bovine red blood cells might be larger, with at least some with an r h > 3.78 nm. This observation and the statistically significant difference between the asymmetric conductance distributions in the N18 neuroblastoma cells and the CHO-K1 cells strongly suggest that: (i) the OlyA/PlyB pores are composed of a varied number of oligomerised units, as has been indicated previously [4] and reported for MACPF/CDC proteins [15][16][17][18][19][20][21][22][23][24] -this is also consistent with our observation that the Renkin's model of the osmoticants transport, assuming a population of equally sized pores [9,11], The conductance (n = 185) was randomly collected from traces obtained at a V HOLD of À60 mV from 10 independent experiments, and the number of single events of pore opening were plotted versus conductance (step-size, 100). (C) Macroscopic current recordings during potential ramps (at 600 mV/s) from the V HOLD of +60 mV to À60 mV: under control conditions, after OlyA/PlyB was transiently applied to the cells for 7 min, and after 2 min application of 1 mM La 3+ .…”

Section: Discussionsupporting

confidence: 79%

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Permeability characteristics of cell‐membrane pores induced by ostreolysin A/pleurotolysin B, binary pore‐forming proteins from the oyster mushroom

et al. 2013

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Edited by Peter BrzezinskiKeywords: Electrophysiology Membrane attack complex/perforin (MACPF) domain Pore-forming protein Pleurotus ostreatus a b s t r a c tProteins from the oyster mushroom, 15 kDa ostreolysin A (OlyA), and 59 kDa pleurotolysin B (PlyB) with a membrane attack complex/perforin (MACPF) domain, damage cell membranes as a binary cytolytic pore-forming complex. Measurements of single-channel conductance and transmembrane macroscopic current reveal that OlyA/PlyB form non-selective ion-conducting pores with broad, skewed conductance distributions in N18 neuroblastoma and CHO-K1 cell membranes. Polyethylene-glycol 8000 (hydrodynamic radius of 3.78 nm) provides almost complete osmotic protection against haemolysis, which strongly suggests a colloid-osmotic type of erythrocyte lysis. Our data indicate that OlyA/PlyB form transmembrane pores of varied sizes, as other pore-forming proteins with a MACPF domain.

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Section: Discussionsupporting

confidence: 91%

“…In contrast to the pores in the N18 neuroblastoma cells, those in the bovine red blood cells might be larger, with at least some with an r h > 3.78 nm. This observation and the statistically significant difference between the asymmetric conductance distributions in the N18 neuroblastoma cells and the CHO-K1 cells strongly suggest that: (i) the OlyA/PlyB pores are composed of a varied number of oligomerised units, as has been indicated previously [4] and reported for MACPF/CDC proteins [15][16][17][18][19][20][21][22][23][24] -this is also consistent with our observation that the Renkin's model of the osmoticants transport, assuming a population of equally sized pores [9,11], The conductance (n = 185) was randomly collected from traces obtained at a V HOLD of À60 mV from 10 independent experiments, and the number of single events of pore opening were plotted versus conductance (step-size, 100). (C) Macroscopic current recordings during potential ramps (at 600 mV/s) from the V HOLD of +60 mV to À60 mV: under control conditions, after OlyA/PlyB was transiently applied to the cells for 7 min, and after 2 min application of 1 mM La 3+ .…”

Section: Discussionsupporting

confidence: 79%

Permeability characteristics of cell‐membrane pores induced by ostreolysin A/pleurotolysin B, binary pore‐forming proteins from the oyster mushroom

et al. 2013

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“…In these investigations, the complement system has been activated by the addition of C5b-6 (reactive lysis), [55][56][57][58] after the addition of antigen and antibody, 53,60 or the adsorption of antibodies onto the membranes. 59 In all cases, an increase in membrane conductance has been observed.…”

Section: Complementmentioning

confidence: 99%

Review: Towards antibacterial strategies: studies on the mechanisms of interaction between antibacterial peptides and model membranes

Wiese

Gutsmann

Seydel

2003

Journal of Endotoxin Research

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Lipopolysaccharides (LPSs) play a dual role as inflammation-inducing and as membrane-forming molecules. The former role attracts significantly more attention from scientists, possibly because it is more closely related to sepsis and septic shock. This review aims to focus the reader's attention to the other role, the function of LPS as the major constituent of the outer layer of the outer membrane of Gram-negative bacteria, in particular those of enterobacterial strains. In this function, LPS is a necessary component of the cell envelope and guarantees survival of the bacterial organism. At the same time, it represents the first target for attacking molecules which may either be synthesized by the host's innate or adaptive immune system or administered to the human body. The interaction of these molecules with the outer membrane may not only directly cause the death of the bacterial organism, but may also lead to the release of LPS into the circulation. Here, we review membrane model systems and their application for the study of molecular mechanisms of interaction of peptides such as those of the human complement system, the bactericidal/permeability-increasing protein (BPI), cationic antibacterial peptide 18 kDa (CAP18) as an example of cathelicidins, defensins, and polymyxin B (PMB). Emphasis is on electrical measurements with a reconstitution system of the lipid matrix of the outer membrane which was established in the authors' laboratory as a planar asymmetric bilayer with one leaflet being composed solely of LPS and the other of the natural phospholipid mixture. The main conclusion, which can be drawn from these investigations, is that LPS and in general its negative charges are the dominant determinants for specific peptide-membrane interactions. However, the detailed mechanisms of interaction, which finally lead to bacterial killing, may involve further steps and differ for different antibacterial peptides.

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“…of MACs, C5b-C9, which are known to act as large-conductance nonspecific ion channels 36 that can initiate lytic or sublytic cell damage. [19][20][21]32,33 In smooth-muscle cells, sublytic concentrations of MACs would act as rogue ion channels, admitting Ca ++ and other ions into the cell and, thereby, initiating vasospasm.…”

mentioning

confidence: 99%

The complement membrane attack complex and the bystander effect in cerebral vasospasm

Park

Ml²,

Jm³

1997

Journal of Neurosurgery

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Activation of complement results in formation of membrane attack complexes (MACs) that can insert themselves either into cells that initiate complement activation or into nearby ("innocent bystander") cells. The MACs form large-conductance, nonspecific ion channels that can cause lytic or sublytic cell damage. The authors used a highly sensitive patch clamp technique to assess the contribution of the bystander effect to the pathophysiology of cerebral vasospasm. They compared the effect of complement activation by autologous aged versus fresh erythrocytes on the membrane conductance of freshly isolated rat cerebral artery smooth-muscle cells. In the presence of autologous serum aged, but not fresh, erythrocytes caused a large increase in membrane conductance, an effect that was prevented by heat-inactivating the serum. Ethyleneglycol tetraacetic acid in the presence of Mg++ attenuated the effect, indicating that complement activation was taking place via the classic pathway. The effect was reproduced by zymosan-activated autologous serum, suggesting that such changes in conductance could result from insertion of MACs secondary to a bystander effect. Both C8- and C9-depleted heterologous sera produced minimal effects that were converted to full effect by addition of the missing complement component. Superoxide dismutase plus catalase did not attenuate the conductance changes produced by autologous serum plus aged erythrocytes. Autologous serum plus aged erythrocyte membrane ghosts that were free of lysate caused a typical increase in conductance. This study demonstrates that complement activation by aged erythrocytes can result in MAC insertion into innocent bystander smooth-muscle cell membranes and that this mechanism, heretofore undescribed, may contribute to development of vasospasm after subarachnoid hemorrhage.

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Formation of ion-conducting channels by the membrane attack complex proteins of complement

Cited by 19 publications

References 31 publications

Permeability characteristics of cell‐membrane pores induced by ostreolysin A/pleurotolysin B, binary pore‐forming proteins from the oyster mushroom

Permeability characteristics of cell‐membrane pores induced by ostreolysin A/pleurotolysin B, binary pore‐forming proteins from the oyster mushroom

Review: Towards antibacterial strategies: studies on the mechanisms of interaction between antibacterial peptides and model membranes

The complement membrane attack complex and the bystander effect in cerebral vasospasm

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