Formation of β-Alanine by Pine Tissues Supplied With Intermediates in Uracil &amp; Orotic Acid Metabolism

Barnes, Robert L.; Naylor, Aubrey W.

doi:10.1104/pp.37.2.171

Cited by 28 publications

(8 citation statements)

References 17 publications

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“…The involvement of intermediates of uracil and orotic acid metabolism in b-alanine biogenesis has still been proposed for a limited number of plant species [4,13,30,31]. The key enzyme of this pathway is b-ureidopropionase [31,32].…”

Section: Discussionmentioning

confidence: 99%

Uracil as one of the multiple sources of β-alanine in Limonium latifolium, a halotolerant β-alanine betaine accumulating Plumbaginaceae

Duhazé

Gagneul

Leport

et al. 2003

Plant Physiology and Biochemistry

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Section: Discussionmentioning

confidence: 99%

Uracil as one of the multiple sources of β-alanine in Limonium latifolium, a halotolerant β-alanine betaine accumulating Plumbaginaceae

Duhazé

Gagneul

Leport

et al. 2003

Plant Physiology and Biochemistry

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“…Moreover, in plants the metabolism of L‐alanine and the non‐proteinogenic amino acid β‐alanine are linked. β‐Alanine biosynthesis can be initiated from four different precursors: (a) the polyamines spermine and spermidine (Galston & Sawhney, ; Rastogi & Davies, ; Terano & Suzuki, ), (b) the nucleotide base uracil (Barnes & Naylor, ; Campbell, ; Traut & Loechel, ), (c) propionate (Hayaishi, Nishizuka, Tatibana, Takeshita, & Kuno, ; Stinson & Spencer, , ), and (d) L‐aspartate (Ottenhof et al, ; Shi, Blundell, & Mizuguchi, ). L‐alanine enters the propionate pathway of β‐alanine synthesis via the reverse reaction of the β‐alanine‐pyruvate aminotransferase [http://www.chem.qmul.ac.uk/iubmb/enzyme/EC:2/6/1/18.html], exchanging an amino group with malonate semialdehyde, and generating pyruvate and β‐alanine (Figure b).…”

Section: Introductionmentioning

confidence: 99%

The Arabidopsis thaliana gene annotated by the locus tag At3g08860 encodes alanine aminotransferase

et al. 2019

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The aminotransferase gene family in the model plant Arabidopsis thaliana consists of 44 genes, eight of which are suggested to be alanine aminotransferases. One of the putative alanine aminotransferases genes, At3g08860, was attributed the function of alanine:glyoxylate aminotransferase/β‐alanine:pyruvate aminotransferase based on the analysis of gene expression networks and homology to other β‐alanine aminotransferases in plants. It was earlier demonstrated that At3g08860 is specifically upregulated in response to osmotic stress, but not other stresses (β‐alanine is an osmoprotectant in plants). Furthermore, it was shown that the expression of At3g08860 is highly coordinated with the genes of the uracil degradation pathway leading to the non‐proteinogenic amino acid β‐alanine. These evidence were suggestive of the involvement of At3g08860 in β‐alanine metabolism. However, direct experimental evidence for the function of At3g08860 was lacking, and therefore, the goal of this study was to elucidate the function of the uncharacterized aminotransferase annotated by the locus tag At3g08860. The cDNA of At3g08860 was demonstrated to functionally complement two E. coli mutants auxotrophic for the amino acids, L‐alanine (proteinogenic) and β‐alanine (non‐proteinogenic). Enzyme activity using purified recombinant At3g08860 further demonstrated that the enzyme is endowed with L‐alanine:glyoxylate aminotransferase activity.

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“…The fates of orotic acid anism. Catabolism of orotic acid proceeds via (Barnes and Naylor 1962), uracil (Barnes and uridine monophosphate to uracil and p-alanine Naylor 1962;Durzan et al 1973), and cytosine (Ross et al 1971). Our study with jack pine (Pandita and Durzan 1971) have been reported, (Pinus banksiana Lamb.)…”

Section: Introductionmentioning

confidence: 99%

Pyrimidine metabolism in seeds and seedlings of jack pine (Pinus banksiana)

Pitel¹,

Durzan²

1975

Can. J. Bot.

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PITEL, J., and D. J. DURZAN. 1975. Pyrimidine metabolism in seeds and seedlings ofjack pine (Pinus banksiana). Can. J. Bot. 53: 673486. Jack pine seedlings rapidly converted radioactive thymine-6-IH to dihydrothymine, p-ureidoisobutyric acid, and P-aminoisobutyric acid. Autoradiographsof hypocotyl cells showed radioactivity largely in the cytoplasm. A small percentage of radioactivity from thymine-2-I4C was salvaged via uracil for the synthesis of RNA. DNA was not labelled significantly. The occurrence of a mechanism to utilize thymidine-methyl-'H for DNA synthesis was detected at low levels in germinating seedlings but was absent in seeds up to 16 h imbibition. Among pyrimidines tested thymidine was the most specific for the labelling of DNA and radioactivity was detected almost entirely over nuclei.The occurrence of an active pathway for the de t i o~~o synthesis of the pyrimidine portions of nucleic acids was demonstrated by the use of orotic acid-4-I4C. Native DNA and all major types of RNA were labelled and radioactivity resided in pyrimidines. Synthesis of DNA involved the conversion of pyrimidine ribonucleotides to deoxyribonucleotides. PITEL, J., et D. J. DURZAN. 1975. Pyrimidine metabolismin seeds and seedlingsofjack pine(Pinus banksiana). Can. J. Bot. 53: 673486. Les semis de pin gris convertirent rapidement la thymine-6-IH radioactive en acide dihydrothymine, P-ur~idoisobutyrique et en acide P-aminoisobutyrique. Les autoradiographies des cellules de I'hypocotyle montrkrent de la radioactivite surtout dans le cytoplasme. Un faible pourcentage de radioactiviti de la thymine-2-I4C fut ricuperi via I'uracil pour la synthkse de I'ARN. L'ADN ne fut pas marque significativement. La prksence d'un mecanisme de recuperation pour utiliser la thymidine-mtthyle--'H pour la synthkse de I'ADN fut detectee a basses teneurs chez les semis en germination mais ce mecanisme etait absent chez les graines imbibees jusqu'i 16 h. Le marquage de I'ADN par la thymidine Ctait le plus specifique parmi les pyrimidines examinees et de la radioactivite fut dCtectCe presque entikrement sur les noyaux.Les auteurs demontrkrent la prksence d'un sentier actif pour la synthkse de notto des portions pyrimidiques des acides nucleiques en utilisant I'acide 0rotique-4-~~C. L'ADN natif et tous les types importants d'ARN furent marquis et la radioactivite se trouvait dans les pyrimidines. La synthkse de I'ADN impliquait la conversion des ribonucleotides de pyrimidine en deoxyribonuclCotides.

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Formation of β-Alanine by Pine Tissues Supplied With Intermediates in Uracil & Orotic Acid Metabolism

Cited by 28 publications

References 17 publications

Uracil as one of the multiple sources of β-alanine in Limonium latifolium, a halotolerant β-alanine betaine accumulating Plumbaginaceae

Uracil as one of the multiple sources of β-alanine in Limonium latifolium, a halotolerant β-alanine betaine accumulating Plumbaginaceae

The Arabidopsis thaliana gene annotated by the locus tag At3g08860 encodes alanine aminotransferase

Pyrimidine metabolism in seeds and seedlings of jack pine (Pinus banksiana)

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