Formulation, characterization and in vitro anti-leishmanial evaluation of amphotericin B loaded solid lipid nanoparticles coated with vitamin B12-stearic acid conjugate

Singh, Aakriti; Yadagiri, Ganesh; Parvez, Shabi; Singh, O. P.; Verma, Anurag; Mudavath, Shyam Lal

doi:10.1016/j.msec.2020.111279

Cited by 40 publications

(37 citation statements)

References 51 publications

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“…The FTIR spectrum for BC: amphotericin B ( Figure 6 B) shows three principal vibrations of the amphotericin B molecule: the band with a maximum at 1730 cm −1 , which can be assigned to the vibration of C=O in the –COOH group, the CH 2 scissoring vibrations (the band centred at 1460 cm −1 ), and the C=C stretching vibration, which is represented by peaks between 1486 and 1631 cm −1 . Additionally, the peaks in the region of 3300–3400 cm −1 indicate stretching vibrations of the O–H group and the N–H stretching in concordance with previous research [ 53 ]. The appearance of peaks between 3400 and 3300 cm −1 in the FTIR spectra, Figure 5 B, can be also attributed as a band of OH groups of BC involved in forming hydrogen bonds with the antifungal agent, indicating an interaction between BC and amphotericin B.…”

Section: Resultssupporting

confidence: 91%

The Mould War: Developing an Armamentarium against Fungal Pathogens Utilising Thymoquinone, Ocimene, and Miramistin within Bacterial Cellulose Matrices

et al. 2021

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An increase in antifungal resistance has seen a surge in fungal wound infections in patients who are immunocompromised resulting from chemotherapy, disease, and burns. Human pathogenic fungi are increasingly becoming resistant to a sparse repertoire of existing antifungal drugs, which has given rise to the need to develop novel treatments for potentially lethal infections. Bacterial cellulose (BC) produced by Gluconacetobacter xylinus has been shown to possess many properties that make it innately useful as a next-generation biopolymer to be utilised as a wound dressing. The current study demonstrates the creation of a pharmacologically active wound dressing by loading antifungal agents into a biopolymer hydrogel to produce a novel wound dressing. Amphotericin B is known to be highly hepatotoxic, which reduces its appeal as an antifungal drug, especially in patients who are immunocompromised. This, coupled with an increase in antifungal resistance, has seen a surge in fungal wound infections in patients who are immunodeficient due to chemotherapy, disease, or injury. Antifungal activity was conducted via Clinical & Laboratory Standards Institute (CLSI) M27, M38, M44, and M51 against Candida auris, Candida albicans, Aspergillus fumigatus, and Aspergillus niger. This study showed that thymoquinone has a comparable antifungal activity to amphotericin B with mean zones of inhibition of 21.425 ± 0.925 mm and 22.53 ± 0.969 mm, respectively. However, the mean survival rate of HEp-2 cells when treated with 50 mg/L amphotericin B was 29.25 ± 0.854% compared to 71.25 ± 1.797% when treated with 50 mg/L thymoquinone. Following cytotoxicity assays against HEp-2 cells, thymoquinone showed a 71.25 ± 3.594% cell survival, whereas amphotericin B had a mean cell survival rate of 29.25 ± 1.708%. The purpose of this study was to compare the efficacy of thymoquinone, ocimene, and miramistin against amphotericin B in the application of novel antifungal dressings.

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Section: Resultssupporting

confidence: 91%

The Mould War: Developing an Armamentarium against Fungal Pathogens Utilising Thymoquinone, Ocimene, and Miramistin within Bacterial Cellulose Matrices

et al. 2021

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“…Chalasani and co-workers produced dextran nanoparticles subsequently modified to provide carboxylate groups for linkage to amino VitB12 derivates [ 92 ]. Equally, solid lipid nanoparticles coated with VitB12-stearic acid conjugate have been produced to encapsulate amphotericin B to improve the low oral dose treatment against visceral leishmaniasis [ 93 ]. VitB12, besides improving absorption through the exploitation of the receptor-mediated uptake, is also used to enhance the solubility of components of nanoparticles [ 94 ].…”

Section: Nanotechnologies and Vitb12mentioning

confidence: 99%

Current Nanocarrier Strategies Improve Vitamin B12 Pharmacokinetics, Ameliorate Patients’ Lives, and Reduce Costs

et al. 2021

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Vitamin B12 (VitB12) is a naturally occurring compound produced by microorganisms and an essential nutrient for humans. Several papers highlight the role of VitB12 deficiency in bone and heart health, depression, memory performance, fertility, embryo development, and cancer, while VitB12 treatment is crucial for survival in inborn errors of VitB12 metabolism. VitB12 is administrated through intramuscular injection, thus impacting the patients’ lifestyle, although it is known that oral administration may meet the specific requirement even in the case of malabsorption. Furthermore, the high-dose injection of VitB12 does not ensure a constant dosage, while the oral route allows only 1.2% of the vitamin to be absorbed in human beings. Nanocarriers are promising nanotechnology that can enable therapies to be improved, reducing side effects. Today, nanocarrier strategies applied at VitB12 delivery are at the initial phase and aim to simplify administration, reduce costs, improve pharmacokinetics, and ameliorate the quality of patients’ lives. The safety of nanotechnologies is still under investigation and few treatments involving nanocarriers have been approved, so far. Here, we highlight the role of VitB12 in human metabolism and diseases, and the issues linked to its molecule properties, and discuss how nanocarriers can improve the therapy and supplementation of the vitamin and reduce possible side effects and limits.

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“…Lipid-based formulations can decrease drug toxicity and improve bioavailability due to its unique physiological and biodegradable properties ( Severino et al, 2012 ). SLNs are widely used as drug delivery systems in cancer and several parasitic diseases like leishmaniasis, malaria, human African trypanosomiasis, and tuberculosis ( Sun et al, 2019 ); ( Singh et al, 2020b ). Dihydroartemisinin-loaded SLNs have been synthesized by single emulsion solvent evaporation technique, characterized and tested for antimalarial activity for overcoming the problems (poor water solubility, poor pharmacokinetic and pharmacodynamics properties) associated with dihydroartemisinin.…”

Section: Nanotechnological Approaches For Malaria Therapeuticsmentioning

confidence: 99%

Current challenges and nanotechnology-based pharmaceutical strategies for the treatment and control of malaria

Gujjari

Kalani

Pindiprolu

et al. 2022

Parasite Epidemiology and Control

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Formulation, characterization and in vitro anti-leishmanial evaluation of amphotericin B loaded solid lipid nanoparticles coated with vitamin B12-stearic acid conjugate

Cited by 40 publications

References 51 publications

The Mould War: Developing an Armamentarium against Fungal Pathogens Utilising Thymoquinone, Ocimene, and Miramistin within Bacterial Cellulose Matrices

The Mould War: Developing an Armamentarium against Fungal Pathogens Utilising Thymoquinone, Ocimene, and Miramistin within Bacterial Cellulose Matrices

Current Nanocarrier Strategies Improve Vitamin B12 Pharmacokinetics, Ameliorate Patients’ Lives, and Reduce Costs

Current challenges and nanotechnology-based pharmaceutical strategies for the treatment and control of malaria

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