Formulation of Drosophila Food for Various Feeding Studies

In Vivo Toxicological Analysis of MnFe2O4@poly(tBGE-alt-PA) Composite as a Hybrid Nanomaterial for Possible Biomedical Use

“…The flies were released to fresh food vials in a ratio of 5:3 (females and males, respectively). They were nurtured under optimum conditions of 60% relative humidity, 25 °C constant temperature, and 12 h day–night condition. , …”

“…Once the food was solidified, adult Oregon-R flies were freed to each vial. Larvae in their third instar of development and adult flies were employed extensively in the research. , Adult flies fertilized with both control and treatment foods and laid eggs. The larvae were utilized in the experiment just 4 to 5 days after hatching. , …”

“…Climbing reflects the neurodegeneration in the Drosophila model. Adult fruit flies’ locomotory behavior was evaluated using this same technique as in a reported protocol . 3-day old flies (30 adult flies) were moved to the climbing apparatus from three distinct concentrations. , Flies were taped gently to the bottom of the vial, and the duration of 10 s to climb 16 cm of the tube was recorded.…”

“…After 5 min, we removed the lid and tallied the larvae in each section. Each batch of larvae performed the test three times, and the experiment was conducted in three sets. ,, …”

“… 22 , 23 Propionic acid and methyl-paraben were added to the food to protect them from fungal and microbial contamination. 22 The flies were released to fresh food vials in a ratio of 5:3 (females and males, respectively). They were nurtured under optimum conditions of 60% relative humidity, 25 °C constant temperature, and 12 h day–night condition.…”