Fosfomycin susceptibility of canine methicillin-resistant Staphylococcus pseudintermedius isolates

DiCicco, Matthew; Weese, Scott; Neethirajan, Suresh; Rousseau, Joyce D.; Singh, Ameet

doi:10.1016/j.rvsc.2014.02.004

Cited by 13 publications

(12 citation statements)

References 24 publications

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“…Studies that exclusively used dru typing ( n = 5) to characterize S. pseudintermedius strains were not included. These steps yielded 58 studies (Figure 1 ), including 49 studies on MRSP (with or without MSSP; Bannoehr et al, 2007 ; Descloux et al, 2008 ; Black et al, 2009 , 2011 ; Moodley et al, 2009 , 2013 ; Kadlec et al, 2010 , 2015 , 2016 ; Perreten et al, 2010 , 2013 ; Ruscher et al, 2010 ; Stegmann et al, 2010 ; Boost et al, 2011 ; Gómez-Sanz et al, 2011 , 2013a , b , 2014 ; Laarhoven et al, 2011 ; Paul et al, 2011 ; DiCicco et al, 2012 , 2014 ; Feng et al, 2012 ; Osland et al, 2012 ; Walther et al, 2012 ; Wang et al, 2012 ; Bardiau et al, 2013 ; Haenni et al, 2013 , 2014 ; Pilla et al, 2013 ; Quitoco et al, 2013 ; Solyman et al, 2013 ; Youn et al, 2013 ; Chanchaithong et al, 2014 , 2015 ; Couto et al, 2014 , 2016 ; Davis et al, 2014 ; Grönthal et al, 2014 , 2015 ; Savini et al, 2014 ; Starlander et al, 2014 ; Börjesson et al, 2015 ; Kjellman et al, 2015 ; McCarthy et al, 2015 ; Rota et al, 2015 ; Damborg et al, 2016 ; Duim et al, 2016 ; Ishihara et al, 2016 ) and 9 studies on MSSP only (Ben Zakour et al, 2011 ; Tse et al, 2011 ; Gharsa et al, 2013 , 2015 ; Matanovic et al, 2013 ; Paul et al, ...…”

Section: Methodsmentioning

confidence: 99%

Systematic Review on Global Epidemiology of Methicillin-Resistant Staphylococcus pseudintermedius: Inference of Population Structure from Multilocus Sequence Typing Data

et al. 2016

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Background and rationale: Methicillin-resistant Staphylococcus pseudintermedius (MRSP) is a major cause of infections in dogs, also posing a zoonotic risk to humans. This systematic review aimed to determine the global epidemiology of MRSP and provide new insights into the population structure of this important veterinary pathogen.Methodology: Web of Science was searched systematically for articles reporting data on multilocus sequence typing (MLST) of S. pseudintermedius isolates from dogs or other animal or human patients and carriers. Data from the eligible studies were then integrated with data from the MLST database for this species. Analysis of MLST data was performed with eBURST and ClonalFrame, and the proportion of MRSP isolates resistant to selected antimicrobial drugs was determined for the most predominant clonal complexes.Results: Fifty-eight studies published over the last 10 years were included in the review. MRSP represented 76% of the 1428 isolates characterized by the current MLST scheme. The population of S. pseudintermedius was highly diverse and included five major MRSP clonal complexes (CCs). CC71, previously described as the epidemic European clone, is now widespread worldwide. In Europe, CC258, which is more frequently susceptible to enrofloxacin and aminoglycosides, and more frequently resistant to sulphonamides/trimethoprim than CC71, is increasingly reported in various countries. CC68, previously described as the epidemic North American clone, is frequently reported in this region but also in Europe, while CC45 (associated with chloramphenicol resistance) and CC112 are prevalent in Asia. It was estimated that clonal diversification in this species is primarily driven by homologous recombination (r/m = 7.52).Conclusion: This study provides evidence that S. pseudintermedius has an epidemic population structure, in which five successful MRSP lineages with specific traits regarding antimicrobial resistance, genetic diversity and geographical distribution have emerged upon a weakly clonal background through acquisition of SCCmec and other mobile genetic elements.

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Section: Methodsmentioning

confidence: 99%

Systematic Review on Global Epidemiology of Methicillin-Resistant Staphylococcus pseudintermedius: Inference of Population Structure from Multilocus Sequence Typing Data

et al. 2016

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“…Tet38 is a known chromosomally encoded native efflux pump in S. aureus, which can confer resistance if (additionally) plasmid encoded [22]. The fosB gene also does not always confer phenotypic resistance [23,24] and is postulated to influence cellular processes, including adaptation to reactive oxygen species [25], while the drug fosfomycin is also known to be naturally produced by Streptomyces spp. [26] and Pseudomonas spp.…”

Section: Discussionmentioning

confidence: 99%

Canine Staphylococcus argenteus: Case Report from The Netherlands

Meijer

Renssen²,

Maat

et al. 2022

Pathogens

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Staphylococcus argenteus has been reported worldwide in humans, while reported non-human cases are sparse. Its complete epidemiology, alongside its infectivity and pathogenicity in humans and non-humans, remain to be clarified. Here, we describe the first reported canine Staphylococcus argenteus, causing a deep wound infection in a Labrador retriever after orthopedic surgery. The closed genome is reported, with phylogenic and genetic analyses, as well as extensive phenotypic antimicrobial susceptibility testing for human and veterinary antibiotics. No genetic explanation could be found for its interaction with a canine host, underscoring the intrinsic multispecies pathogenicity and potential (anthropo-)zoonotic spread of Staphylococcus argenteus.

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“…On the contrary, we found a high percentage of isolates exhibiting susceptibility to Imipenem, Ampicillin/Sulbactam, Rifampicin, and Cephalexin ( Table 2 ). Other studies reported variable degrees of susceptibility of this genus to synthetic thiazole compounds ( 44 ), cationic peptides ( 45 ), naturals antimicrobial peptides and cathelicidin ( 46 ), orbifloxacin ( 47 ), marbofloxacin ( 48 ), pradofloxacin ( 49 ), and phosphomicin ( 50 ).…”

Section: Discussionmentioning

confidence: 99%

Molecular Detection and Characterization of the mecA and nuc Genes From Staphylococcus Species (S. aureus, S. pseudintermedius, and S. schleiferi) Isolated From Dogs Suffering Superficial Pyoderma and Their Antimicrobial Resistance Profiles

et al. 2020

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Canine superficial pyoderma (CSP) is a bacterial infection secondary to several skin diseases of the dog. Staphylococcus pseudintermedius, which is a commensal bacterium of the dog's skin, is the leading agent found in dogs affected by CSP, which can progress to deep pyoderma. It is also of clinical significance because S. pseudintermedius strains carry antimicrobial resistance genes, mainly the mecA gene. In this descriptive longitudinal study, molecular characterization of bacterial isolates from dogs affected by CSP was performed in addition to phenotyping, antimicrobial profiling, and assessment of resistance carriage status. Fifty dogs (24 females and 26 males) attending the CES University Veterinary Teaching Hospital were included in the study. CSP was confirmed according to clinical signs and cytological examination. Swabs were taken from active skin lesions for bacterial culture, and phenotyping and antimicrobial resistance profiles were assessed using API-Staph phenotyping and the Kirby-Bauer method, respectively. We also performed molecular detection and characterization of the mecA and nuc encoding gene of coagulase-positive Staphylococci. The mecA gene frequency was established by qPCR amplification of a 131bp gene fragment. Data were evaluated by descriptive statistics. Erythema, peeling, pruritus, and alopecia were the predominant symptoms (72, 56, and 46%, respectively). We isolated bacteria compatible with Staphylococcus species from all samples tested. González-Domínguez et al. mecA/nuc Genes From Staphylococcus Species API phenotyping showed 83.1 to 97.8% compatibility with S. pseudintermedius. PCR-genotyping resulted in 15, 3, and 1 isolates positive for S. pseudintermedius, S. aureus, and S. schleiferi, respectively. Isolated strains showed high susceptibility to Imipenem, Ampicillin/Sulbactam, and Rifampicin (100, 94, and 92%, respectively). The highest resistance was against Vancomycin and Trimethoprim/Sulfamethoxazole (98 and 74%, respectively). S. pseudintermedius, S. aureus, and S. schleiferi isolates were cloned and shared 96% sequence homology. Finally, we found 62% carriage status of the mecA gene in isolates of CSP patients, although only 36% of the isolates were methicillin-resistant. Identification of three Staphylococcus species causing CSP, high-level resistance against conventional antimicrobials, and carriage of the mecA gene highlight the importance of performing molecular characterization of bacteria causing dermatological conditions in dogs.

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Fosfomycin susceptibility of canine methicillin-resistant Staphylococcus pseudintermedius isolates

Cited by 13 publications

References 24 publications

Systematic Review on Global Epidemiology of Methicillin-Resistant Staphylococcus pseudintermedius: Inference of Population Structure from Multilocus Sequence Typing Data

Systematic Review on Global Epidemiology of Methicillin-Resistant Staphylococcus pseudintermedius: Inference of Population Structure from Multilocus Sequence Typing Data

Canine Staphylococcus argenteus: Case Report from The Netherlands

Molecular Detection and Characterization of the mecA and nuc Genes From Staphylococcus Species (S. aureus, S. pseudintermedius, and S. schleiferi) Isolated From Dogs Suffering Superficial Pyoderma and Their Antimicrobial Resistance Profiles

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