Fractal dimension analysis: A new tool for analyzing colony-forming units

Abstract

The gold standard for quantifying bacteria both in routine diagnostics and in research is plating followed by count of colony-forming units (CFU). But, manual CFU counting on plates is time-consuming and subjective. We evaluated fractal dimension as a new methodology for evaluating CFU. Twenty fragments of expanded polytetrafluoroethylene (ePTFE) synthetic vascular prosthesis and 20 silicone prostheses were embedded in bacterial suspensions and incubated. The prostheses were then sown in solid culture medium and incubated for 48 h. Petri dishes were photographed and analyzed by fractal dimension. There was correlation between the number of CFU in manual counting and the fractal dimension analysis ( p = 0.0001). We demonstrated that fractal dimension is a useful method for microbiological analyses in researches. It makes CFU analysis easier and faster and can be used regardless of the culture medium. Petri dishes with different bacterial colonies were photographed with a digital camera under natural light. The images were binarized and analyzed with ImageJ Ⓡ 's “fractal dimension” tool. Fractal dimension analysis showed to be a good tool for evaluating the amount of colony-forming unit.

Spectra of Reduced Fractals and Their Applications in Biology

“…We have also calculated the SRFs and demonstrated that they can be used as a new tool to investigate properties of green algae and also to classify them, based on the form of their SRF, within their families as currently algae of a given family are neither ordered nor organized [26,27]. Moreover, the SRFs can also be generated for other types of algae, namely, Macroalgae (red and brown) or Microalgae [26,27], as well as for many other diversified biological systems, such as the roots of plants [30] and their complexity [34], scaling time in biochemical networks [35], organization of ecosystems [36], human physiology and well-being [37], and microbial colonies [38]. We do hope that biologists working in different areas, and other natural scientists, find the SRFs useful in their work and apply them to different natural systems.…”

Perbandingan Metode Median Filtering dengan CLAHE dalam Mengidentifikasi Koloni Bakteri

“…Teknologi pengolahan citra digital telah banyak dikembangkan untuk membuat aplikasi dalam berbagai bidang, salah satunya adalah pemanfaatan teknologi citra digital dalam bidang biologi untuk analisis citra. Sebelumnya peneliti telah melakukan penelitian serupa yang bertujuan untuk menghitung koloni bakteri dan menghasilkan akurasi sebesar 94% dan peneliti menemukan bahwa beberapa koloni tidak terdeteksi karena terfilter saat preprocessing, sehingga peneliti melakukan penelitian lebih lanjut mengenai preprocessing Kata Kunci-Koloni, Pengolahan Citra, Preprocessing I. PENDAHULUAN Penelitian di bidang mikrobiologi terutama bakteri seringkali berpatokan pada penentuan jumlah koloni atau Colony Forming Units (CFUs) yang akurat [2]. Bakteri dapat ditumbuhkan dalam suatu medium agar dan akan membentuk penampakan berupa koloni.…”

“…Standar emas untuk mengukur bakteri baik dalam diagnostik rutin dan dalam penelitian adalah pelapisan diikuti oleh jumlah unit pembentuk koloni (CFU). Namun, penghitungan CFU manual pada pelat memakan waktu dan subjektif [2]. Selain itu proses manual membutuhkan tingkat konsentrasi yang tinggi, sehingga jumlah koloni yang terhitung sangat dipengaruhi oleh kondisi fisik dan mental dari peneliti bidang mikrobiologi itu sendiri.…”

Affinity of Staphylococcus aureus for prostheses colonization compared to other bacteria. An in vitro study

“…After incubation, the plates were photographed with a digital camera (Cyber Shot W830 20.1MP, Sony, Japan) and analyzed with ImageJ ® software (http://rsbweb.nih.gov/ij/). Fractal dimension analysis was performed by the box-counting method (Figure 1) (Stolze et al, 2019;Nai et al, 2021).…”